Detailed information NTG0514559

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: I8N73_RS13350
• Genome accession: NZ_CP065791
• Coordinates: 2742029..2742643 (-)
• Length: 204 a.a.
• NCBI ID: WP_199022049.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LBUM279
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2737029..2747643

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I8N73_RS13320 (I8N73_13320)	rpsT	2737332..2737598 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
I8N73_RS13325 (I8N73_13325)	holA	2737615..2738656 (-)	1042	Protein_2600	DNA polymerase III subunit delta	-
I8N73_RS13330 (I8N73_13330)	-	2738696..2738847 (-)	152	Protein_2601	hypothetical protein	-
I8N73_RS13335 (I8N73_13335)	-	2738888..2739022 (+)	135	WP_003152870.1	YqzM family protein	-
I8N73_RS13340 (I8N73_13340)	comEC	2739041..2741392 (-)	2352	WP_199022048.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I8N73_RS13345 (I8N73_13345)	-	2741393..2741962 (-)	570	WP_144663971.1	ComE operon protein 2	-
I8N73_RS13350 (I8N73_13350)	comEA	2742029..2742643 (-)	615	WP_199022049.1	helix-hairpin-helix domain-containing protein	Machinery gene
I8N73_RS13355 (I8N73_13355)	comER	2742702..2743523 (+)	822	WP_020956098.1	late competence protein ComER	-
I8N73_RS13360 (I8N73_13360)	-	2743593..2744330 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
I8N73_RS13365 (I8N73_13365)	rsfS	2744327..2744683 (-)	357	WP_007408260.1	ribosome silencing factor	-
I8N73_RS13370 (I8N73_13370)	yqeK	2744701..2745261 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
I8N73_RS13375 (I8N73_13375)	-	2745251..2745820 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
I8N73_RS13380 (I8N73_13380)	yhbY	2745831..2746121 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
I8N73_RS13385 (I8N73_13385)	aroE	2746115..2746957 (-)	843	WP_199022050.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22179.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=514559 I8N73_RS13350 WP_199022049.1 2742029..2742643(-) (comEA) [Bacillus velezensis strain LBUM279]
MTKAMMTERLRRNLKILGACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=514559 I8N73_RS13350 WP_199022049.1 2742029..2742643(-) (comEA) [Bacillus velezensis strain LBUM279]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGGCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363