Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   I8N73_RS13340 Genome accession   NZ_CP065791
Coordinates   2739041..2741392 (-) Length   783 a.a.
NCBI ID   WP_199022048.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM279     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2734041..2746392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N73_RS13305 (I8N73_13305) - 2734406..2734744 (-) 339 WP_095352939.1 YqxA family protein -
  I8N73_RS13310 (I8N73_13310) spoIIP 2734761..2735954 (-) 1194 WP_007612673.1 stage II sporulation protein P -
  I8N73_RS13315 (I8N73_13315) gpr 2736023..2737129 (-) 1107 WP_007408268.1 GPR endopeptidase -
  I8N73_RS13320 (I8N73_13320) rpsT 2737332..2737598 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  I8N73_RS13325 (I8N73_13325) holA 2737615..2738656 (-) 1042 Protein_2600 DNA polymerase III subunit delta -
  I8N73_RS13330 (I8N73_13330) - 2738696..2738847 (-) 152 Protein_2601 hypothetical protein -
  I8N73_RS13335 (I8N73_13335) - 2738888..2739022 (+) 135 WP_003152870.1 YqzM family protein -
  I8N73_RS13340 (I8N73_13340) comEC 2739041..2741392 (-) 2352 WP_199022048.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  I8N73_RS13345 (I8N73_13345) - 2741393..2741962 (-) 570 WP_144663971.1 ComE operon protein 2 -
  I8N73_RS13350 (I8N73_13350) comEA 2742029..2742643 (-) 615 WP_199022049.1 helix-hairpin-helix domain-containing protein Machinery gene
  I8N73_RS13355 (I8N73_13355) comER 2742702..2743523 (+) 822 WP_020956098.1 late competence protein ComER -
  I8N73_RS13360 (I8N73_13360) - 2743593..2744330 (-) 738 WP_015240247.1 class I SAM-dependent DNA methyltransferase -
  I8N73_RS13365 (I8N73_13365) rsfS 2744327..2744683 (-) 357 WP_007408260.1 ribosome silencing factor -
  I8N73_RS13370 (I8N73_13370) yqeK 2744701..2745261 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  I8N73_RS13375 (I8N73_13375) - 2745251..2745820 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  I8N73_RS13380 (I8N73_13380) yhbY 2745831..2746121 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86684.47 Da        Isoelectric Point: 8.7881

>NTDB_id=514557 I8N73_RS13340 WP_199022048.1 2739041..2741392(-) (comEC) [Bacillus velezensis strain LBUM279]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKQYAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIEQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGESRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFGMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVFGGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=514557 I8N73_RS13340 WP_199022048.1 2739041..2741392(-) (comEC) [Bacillus velezensis strain LBUM279]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCTGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGTATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCTGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTGAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCGTATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGGTTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTTCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGGAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGGTATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTAGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCATATTCTATCGGCGAGAAGGTTTTGATTCCATTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTAGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGGGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTTGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAGCAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.995

98.595

0.562