Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I6G32_RS14290 Genome accession   NZ_CP065750
Coordinates   3116168..3118288 (+) Length   706 a.a.
NCBI ID   WP_013981654.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain FDAARGOS_875     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3111168..3123288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G32_RS14270 (I6G32_14270) comM 3113331..3114395 (+) 1065 WP_013981650.1 pilus assembly protein PilM Machinery gene
  I6G32_RS14275 (I6G32_14275) pilN 3114395..3114967 (+) 573 WP_013981651.1 type 4a pilus biogenesis protein PilN -
  I6G32_RS14280 (I6G32_14280) pilO 3114964..3115590 (+) 627 WP_017244292.1 type 4a pilus biogenesis protein PilO -
  I6G32_RS14285 (I6G32_14285) pilP 3115587..3116114 (+) 528 WP_013981653.1 type 4a pilus biogenesis lipoprotein PilP -
  I6G32_RS14290 (I6G32_14290) pilQ 3116168..3118288 (+) 2121 WP_013981654.1 type IV pilus secretin PilQ Machinery gene
  I6G32_RS14295 (I6G32_14295) aroK 3118292..3118837 (+) 546 WP_080564967.1 shikimate kinase AroK -
  I6G32_RS14300 (I6G32_14300) aroB 3118861..3119964 (+) 1104 WP_013981656.1 3-dehydroquinate synthase -
  I6G32_RS14305 (I6G32_14305) - 3119975..3121564 (+) 1590 WP_013981657.1 AAA family ATPase -

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 75749.00 Da        Isoelectric Point: 4.7402

>NTDB_id=513947 I6G32_RS14290 WP_013981654.1 3116168..3118288(+) (pilQ) [Stutzerimonas stutzeri strain FDAARGOS_875]
MNTTLSRLGVSLLAAFISPALLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGESSGAANPVSASAAITAPLVKPAPVKPFAAGKAIDN
IDFRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVVFAKAELPEALRVRLDVQDFATPVKFVDSTAQAGGASIAIEPTGR
YDYLAYQTDNKLTISVKPLTEEEADKRKTDRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTSNGAV
AEDRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKALGVRWGGSYTNDSWGLYGKDGA
IGRDEDSGRYLLPGTDKVGNFKLEDALAPVPFVDLGVLNSTAGIGIGYVSDHGILDLQLSAMEKTGNGEIVSQPKVVTAD
KETAKILKGAEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFANALNGVPPINKNEVNAKVLV
TDGETIVIGGVFSNSQISSIEKVPFLGDVPFVGRLFRRDIVEDKKSELLVFLTPRILNYRAVAVGQ

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=513947 I6G32_RS14290 WP_013981654.1 3116168..3118288(+) (pilQ) [Stutzerimonas stutzeri strain FDAARGOS_875]
ATGAACACTACCCTTTCTCGCCTGGGCGTATCTCTGCTGGCGGCATTTATTTCGCCAGCACTGCTGGCGGCCAATCTCAA
CGCCTTGGATGTGGCTGCTTTGCCGGGCGATCGGGTTGAGTTGAAACTGGCATTTGACGAGCCTGTCGCGTCACCTCGTG
GCTACACGCTGGATCAGCCCGCGCGCATTGCCCTGGATCTTCCAGGCGTATCCAACAACCTGGGGGCCAAAAATCGGGAG
CTGGGCGTTGGTAATGCACGCAGCGTAACGGTCGTCGAGGCTCAAGGGCGCACCCGGCTCATCGTCAATCTGACCGCGCT
TGTTCCGTATTCCACTCGAGTGGACGGGAACAATCTGTTTGTCGTGCTGGGAGAAAGTTCGGGAGCGGCCAACCCTGTGT
CTGCATCCGCCGCGATCACTGCTCCGCTTGTCAAGCCAGCCCCGGTAAAGCCGTTTGCGGCCGGCAAGGCGATCGACAAT
ATCGATTTTCGCCGCGGTGAGGATGGTGCCGGAAACGTGGTAATCACACTGTCCGATCCGTCGGTAGGTTCGACTATCGA
AGAGCAGGGTGGAAAGATCCGCGTTGTGTTCGCCAAGGCGGAGCTGCCTGAAGCACTGCGTGTTCGGCTCGACGTCCAGG
ATTTTGCTACGCCGGTGAAGTTTGTCGACTCGACCGCTCAGGCAGGCGGCGCCAGTATCGCCATCGAGCCTACAGGGCGA
TACGATTATCTCGCCTATCAGACAGACAACAAGCTGACCATCAGCGTAAAGCCCCTTACAGAAGAAGAGGCCGATAAGCG
CAAGACAGACCGTTTTGCCTATTCCGGTGAAAAACTGTCGCTGAACTTCCAGGATATTGATGTTCGTTCCGTACTCCAGT
TGATCGCTGATTTTACTGACCTGAACTTGGTTGCCAGCGACACCGTCAGTGGCAACATCACCCTTCGTTTGCAGAACGTG
CCTTGGGACCAAGCGCTCGATCTGGTTTTGAAAACCAAGGGGCTGGATAAGCGGCAAGTAGGGAACGTGCTGCTCGTTGC
GCCAGCTGACGAAATCGCTGCGCGTGAGCGCCAAGAGCTCGAGTCGCAGCGCCAGATTGCCGAGCTTGCACCGTTGCGTC
GTGAGGTCGTGCAAGTCAACTATGCCAAGGCTGCGGACATTGCTCGCCTGTTCCAGTCAGTGACGAGCAACGGCGCCGTT
GCAGAGGATCGTGGCTCTATAGCGGTGGATGATCGCACCAATAACATCATCGCTTATCAAACGCAGGAGCGACTTGACGA
GCTGCGTCGCATCGTCGCTCAGCTCGACATTCCTGTGCGGCAAGTGATGATTGAGGCGCGCATTGTTGAGGCGAATGTGG
GCTACGACAAAGCGCTTGGGGTTCGTTGGGGGGGGAGCTACACCAACGACAGCTGGGGCTTGTATGGCAAGGACGGTGCC
ATCGGCCGTGATGAAGATAGCGGTCGATATCTTCTCCCAGGTACAGACAAAGTCGGCAATTTTAAGCTTGAAGATGCGTT
GGCCCCGGTTCCATTTGTCGATCTAGGAGTGCTTAACAGCACCGCCGGCATTGGGATCGGCTATGTCTCGGACCATGGAA
TCCTGGACCTTCAATTGTCGGCTATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCAAAGGTGGTTACTGCGGAT
AAAGAAACGGCCAAGATCTTGAAAGGTGCGGAAATCCCCTACCAAGAAGCCAGTTCGAGTGGCGCAACGTCGACATCCTT
CAAGGAGGCAGCTCTTTCGCTTGAGGTGACGCCGCAGATCACGCCGGACAACCGCATTATCATGGAGGTCAAGGTCACCA
AGGATGCCCCTGATTTTGCTAACGCTCTGAATGGCGTGCCGCCGATCAACAAGAACGAAGTCAACGCCAAAGTTCTTGTT
ACCGACGGAGAAACAATCGTCATCGGTGGTGTATTCTCCAACAGCCAGATCAGCAGCATTGAAAAAGTGCCGTTTCTGGG
CGACGTTCCGTTCGTTGGTCGCCTCTTCCGACGGGACATCGTGGAAGATAAAAAATCCGAGCTTCTGGTCTTCTTGACCC
CTAGAATCCTCAACTACCGAGCGGTCGCCGTGGGCCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(541-697)

AMIN domain (AMIN)

(38-123)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.992

100

0.754
  comQ Acinetobacter baylyi ADP1

41.763

98.017

0.409
  pilQ Acinetobacter baumannii strain A118

40.112

100

0.405
  pilQ Acinetobacter baumannii D1279779

40.112

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.257

95.892

0.367
  pilQ Legionella pneumophila strain Lp02

38.109

95.892

0.365