Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   I6G22_RS03110 Genome accession   NZ_CP065737
Coordinates   565843..566490 (-) Length   215 a.a.
NCBI ID   WP_023188531.1    Uniprot ID   A0AAW7IZF3
Organism   Lactococcus lactis strain FDAARGOS_865     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560843..571490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G22_RS03085 (I6G22_03085) atpF 560912..561418 (-) 507 WP_025016982.1 F0F1 ATP synthase subunit B -
  I6G22_RS03090 (I6G22_03090) atpB 561433..562146 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  I6G22_RS03095 (I6G22_03095) - 562191..562406 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  I6G22_RS03100 (I6G22_03100) - 562591..563367 (-) 777 WP_023188533.1 alpha/beta hydrolase -
  I6G22_RS03105 (I6G22_03105) comEC 563652..565862 (-) 2211 WP_025016983.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  I6G22_RS03110 (I6G22_03110) comEA 565843..566490 (-) 648 WP_023188531.1 ComEA family DNA-binding protein Machinery gene
  I6G22_RS03115 (I6G22_03115) - 566550..567911 (-) 1362 WP_025016984.1 ABC transporter permease -
  I6G22_RS03120 (I6G22_03120) - 567908..568840 (-) 933 WP_025016985.1 ABC transporter ATP-binding protein -
  I6G22_RS03125 (I6G22_03125) - 568945..569343 (-) 399 WP_025016986.1 hypothetical protein -
  I6G22_RS03130 (I6G22_03130) - 569447..570010 (-) 564 WP_004255233.1 GNAT family N-acetyltransferase -
  I6G22_RS03135 (I6G22_03135) - 570183..571361 (-) 1179 WP_012898345.1 SLC13 family permease -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23624.76 Da        Isoelectric Point: 4.8659

>NTDB_id=513712 I6G22_RS03110 WP_023188531.1 565843..566490(-) (comEA) [Lactococcus lactis strain FDAARGOS_865]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDIIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=513712 I6G22_RS03110 WP_023188531.1 565843..566490(-) (comEA) [Lactococcus lactis strain FDAARGOS_865]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATATAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

75.688

100

0.767
  comEA/celA/cilE Streptococcus mitis SK321

44.907

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.578

100

0.442
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.553

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae D39

41.553

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae R6

41.553

100

0.423
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.743

100

0.423
  comEA Latilactobacillus sakei subsp. sakei 23K

36.564

100

0.386