Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G34_RS10055 Genome accession   NZ_CP065720
Coordinates   2137949..2139652 (+) Length   567 a.a.
NCBI ID   WP_102840585.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain FDAARGOS_877     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2132949..2144652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G34_RS10025 (I6G34_10025) - 2134034..2134930 (-) 897 WP_102840582.1 ABC transporter ATP-binding protein -
  I6G34_RS10030 (I6G34_10030) - 2134927..2135670 (-) 744 WP_232244584.1 hypothetical protein -
  I6G34_RS10035 (I6G34_10035) - 2135766..2136575 (-) 810 WP_146030731.1 ABC transporter permease subunit -
  I6G34_RS21035 (I6G34_10040) - 2136638..2136901 (-) 264 WP_102840583.1 type II secretion system protein -
  I6G34_RS10045 (I6G34_10045) - 2136936..2137376 (-) 441 WP_146030732.1 hypothetical protein -
  I6G34_RS20990 (I6G34_10050) - 2137392..2137577 (-) 186 WP_054093589.1 type IV pilin protein -
  I6G34_RS10055 (I6G34_10055) pilB 2137949..2139652 (+) 1704 WP_102840585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G34_RS10060 (I6G34_10060) pilC 2139655..2140875 (+) 1221 WP_063544459.1 type II secretion system F family protein Machinery gene
  I6G34_RS10065 (I6G34_10065) pilD 2140879..2141745 (+) 867 WP_102840586.1 A24 family peptidase Machinery gene
  I6G34_RS10070 (I6G34_10070) coaE 2141878..2142486 (+) 609 WP_102840587.1 dephospho-CoA kinase -
  I6G34_RS10075 (I6G34_10075) yacG 2142483..2142680 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  I6G34_RS10080 (I6G34_10080) - 2142705..2143385 (-) 681 WP_102840589.1 energy-coupling factor ABC transporter permease -
  I6G34_RS10085 (I6G34_10085) - 2143424..2144461 (-) 1038 WP_102840590.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62258.22 Da        Isoelectric Point: 5.3387

>NTDB_id=513463 I6G34_RS10055 WP_102840585.1 2137949..2139652(+) (pilB) [Stutzerimonas frequens strain FDAARGOS_877]
MNENAPLGGLARQLVLTGQLDEKIAQQAQQQALRNQVPLVTWLVQNKLVKSRVLVDIAGEQFGVPFFDLGALDKETQPKE
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATSSLEGLNDSELD
GLDIEVAGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLSEGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=513463 I6G34_RS10055 WP_102840585.1 2137949..2139652(+) (pilB) [Stutzerimonas frequens strain FDAARGOS_877]
ATGAACGAAAATGCGCCCCTCGGCGGTCTGGCCCGCCAGCTGGTTCTTACCGGCCAGCTCGACGAAAAAATCGCACAGCA
GGCCCAGCAGCAAGCCTTGCGTAACCAAGTGCCTTTGGTGACTTGGTTGGTGCAGAACAAGCTGGTCAAAAGTCGGGTGC
TTGTCGATATCGCCGGTGAGCAGTTTGGTGTTCCGTTCTTTGATCTGGGGGCGCTGGACAAAGAAACCCAGCCGAAGGAA
CTGGTCAGCGAGAAGCTGGTGCGCCAGCACCGAGCTCTGCCGTTGCAGCGACGAGGCAACAAGCTTTATGTGGCGGTGTC
CGATCCAACCAATCATCAGGCGATCAGTGATATTCAGTTCAATACGGGGCTTTCCGTTGAGGCTGTGCTGGTTGAGGACG
ACAAGCTCGGTGAGGCCATCGAAAAGTTCTTCGAGTCGGCGACCTCGTCCCTCGAAGGATTGAACGATTCCGAGTTGGAT
GGCTTGGATATCGAAGTGGCCGGTGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCCGATGATGCGCCAGTGGTGCG
GTTTGTTAACAAGATGCTGCTCGATGCGATCAAGGGTGGTTCGTCCGATTTGCACTTCGAGCCATATGAAAAGAGCTATC
GGGTGCGCTTTCGTACGGACGGCATGTTGCACGAGGTTGCCCGCCCGCCGGTCCAGTTGGTCAGCCGTATCTCAGCGCGT
CTGAAAGTAATGGCCAGTCTGGATATCGCCGAGCGGCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGGTCTCGAGCAC
CAAGTCGATCGATTTTCGCGTGAATACGCTACCCACCCTGTGGGGCGAGAAGATTGTTATGCGAATCTTGGATTCTTCCA
GTGCCAAGATGGGTATTGATGCGCTGGGGTATGAGGATGACCAGAAGGAGCTCTATCTAAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGCCCGACAGGCTCGGGCAAGACTGTCTCGCTTTATACCGGCCTGAATATTCTCAATACGGT
CGATGTGAATATCTCCACTGCGGAGGATCCGGTAGAGATCAATTTGGAAGGTATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTTGCGCAGGCGCTTCGAGCGTTCCTGCGCCAGGATCCGGACATCATCATGGTGGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACGCGCTTGCGCAACATGGGGGTGCCGGCATTCAACATTGCCACATCAGTCAACCTGATCATTGCTCAGC
GGCTGGCTCGTAAGCTGTGCCCCAGCTGCAAACAAGAGGTCGATGTGCCGCGAGATGTTCTGCTCAGCGAAGGATTTCCT
GAAGCGCGAATCGGTTCCTTCAAGCTTTACGGGCCGGTGGGGTGCGATAGCTGCAAGGGAGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAAAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTCAAGGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.142

99.118

0.596
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

54.991

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

51.099

96.296

0.492
  pilB Vibrio cholerae strain A1552

54.102

90.3

0.489
  pilB Vibrio campbellii strain DS40M4

50

97.707

0.489
  pilF Neisseria gonorrhoeae MS11

50.829

95.767

0.487
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.566

93.474

0.379
  pilF Thermus thermophilus HB27

37.852

100

0.379