Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G96_RS02275 Genome accession   NZ_CP065627
Coordinates   500583..502325 (+) Length   580 a.a.
NCBI ID   WP_197944164.1    Uniprot ID   -
Organism   Delftia acidovorans strain FDAARGOS_939     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495583..507325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G96_RS02245 (I6G96_02245) proB 496101..497249 (-) 1149 WP_012207281.1 glutamate 5-kinase -
  I6G96_RS02250 (I6G96_02250) cgtA 497333..498448 (-) 1116 WP_012207282.1 Obg family GTPase CgtA -
  I6G96_RS02255 (I6G96_02255) rpmA 498598..498855 (-) 258 WP_012207283.1 50S ribosomal protein L27 -
  I6G96_RS02260 (I6G96_02260) rplU 498871..499182 (-) 312 WP_012207284.1 50S ribosomal protein L21 -
  I6G96_RS02265 (I6G96_02265) - 499413..500342 (+) 930 WP_034396917.1 polyprenyl synthetase family protein -
  I6G96_RS02275 (I6G96_02275) pilB 500583..502325 (+) 1743 WP_197944164.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G96_RS02280 (I6G96_02280) pilC 502364..503581 (+) 1218 WP_065345744.1 type II secretion system F family protein Machinery gene
  I6G96_RS02285 (I6G96_02285) pilD 503581..504468 (+) 888 WP_197944165.1 prepilin peptidase Machinery gene
  I6G96_RS02290 (I6G96_02290) coaE 504483..505127 (+) 645 WP_197944166.1 dephospho-CoA kinase -
  I6G96_RS02295 (I6G96_02295) zapD 505217..505972 (+) 756 WP_096913920.1 cell division protein ZapD -
  I6G96_RS02300 (I6G96_02300) yacG 505972..506220 (+) 249 WP_414017743.1 DNA gyrase inhibitor YacG -
  I6G96_RS02305 (I6G96_02305) - 506231..506749 (-) 519 WP_197944168.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62699.82 Da        Isoelectric Point: 5.3458

>NTDB_id=512278 I6G96_RS02275 WP_197944164.1 500583..502325(+) (pilB) [Delftia acidovorans strain FDAARGOS_939]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AVNAARLPHELLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASEA
MESYSGGGDFEFGDLKPDEVAEEKDNTAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLIEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLEAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=512278 I6G96_RS02275 WP_197944164.1 500583..502325(+) (pilB) [Delftia acidovorans strain FDAARGOS_939]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGCATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTATCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCGGGCATCCTCTCTGCCCTGGAAGTGGCCGAGACGGTCTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCGTCAACGCCGCGCGCCTGCCGCATGAGTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCCTTGAGCAA
GCGCGGCAACCGCATCACGGTGGCCACGGCCGACCCGACACAGCAGGAAGCTGCAGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAGGCCGACAAGCTGGAACGCCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAGCC
ATGGAGAGCTACAGCGGGGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAGAAGGACAACAC
GGCGGCAGCGGACGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCCTACGAACATCAGTACCGCGTGCGCTTCCGGATTGACGGTGAGTTGCGCGAGATTGCATCG
CCCCCCATTGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGGGTTCC
GCAAGACGGGCGCATGAAGCTCAAGGTGGGTCCGGACCGCGTCATCGATTTTCGCGTGAGCACCCTGCCCACGCTCTTCG
GCGAGAAGATCGTGATCCGTATCCTTGACCCGAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCCGAGGAA
AAGGCTCGCCTGATTGAAGCCATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCGACGGCAGAAGACCCCTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTAAACGAAAAGGCGGGCCTTACCTTTGCGACGGCGCTCAAGGCCTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCA
CTTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCACTGCTGGAGGCGGGCTATGACGAGGCGGACATCGATGGCAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAGGCATGCAACAACGGCTACAAGGGACGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAAGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGCGCACTGGAGATCGCGGCACAGGCCAAGCGTGAAGGCGTGCGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.206

97.241

0.547
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

50.264

98.103

0.493
  pilB Vibrio cholerae strain A1552

46.279

95

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.372

95

0.431
  pilB Vibrio campbellii strain DS40M4

43.54

97.414

0.424
  pilF Thermus thermophilus HB27

38.028

97.931

0.372