Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I5961_RS02050 Genome accession   NZ_CP065410
Coordinates   451246..453324 (+) Length   692 a.a.
NCBI ID   WP_085698118.1    Uniprot ID   -
Organism   Pseudomonas sp. IAC-BECa141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446246..458324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5961_RS02030 (I5961_02030) comM 448460..449524 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  I5961_RS02035 (I5961_02035) - 449524..450090 (+) 567 WP_085698115.1 PilN domain-containing protein -
  I5961_RS02040 (I5961_02040) pilO 450087..450710 (+) 624 WP_085698116.1 type 4a pilus biogenesis protein PilO -
  I5961_RS02045 (I5961_02045) - 450707..451231 (+) 525 WP_227234185.1 pilus assembly protein PilP -
  I5961_RS02050 (I5961_02050) pilQ 451246..453324 (+) 2079 WP_085698118.1 type IV pilus secretin PilQ Machinery gene
  I5961_RS02055 (I5961_02055) aroK 453329..453847 (+) 519 WP_011332081.1 shikimate kinase AroK -
  I5961_RS02060 (I5961_02060) aroB 454000..455100 (+) 1101 WP_085698119.1 3-dehydroquinate synthase -
  I5961_RS02065 (I5961_02065) - 455111..456712 (+) 1602 WP_227234187.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75140.38 Da        Isoelectric Point: 5.5748

>NTDB_id=509227 I5961_RS02050 WP_085698118.1 451246..453324(+) (pilQ) [Pseudomonas sp. IAC-BECa141]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLTSKTRD
LGSGNARTVTVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKVAAPRTAARAPRAAPVAAPVRTFTPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIMLNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FEYSTYQTDNKLTVSIRPLTIDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=509227 I5961_RS02050 WP_085698118.1 451246..453324(+) (pilQ) [Pseudomonas sp. IAC-BECa141]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGCTGAAGCTGTCGTTCGACGGCCCGCCCCCGCAACCTAAAG
GCTACACCACCGATTCGCCGGCAAGGATCGCCCTCGATCTGCCGGGGGTGGCCAGTCAGCTGACGAGCAAGACTCGCGAC
CTGGGCAGCGGCAATGCCCGCACGGTCACGGTAGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCACCTTACGACACCCGGGTCGAAGGCAATACGGTGTTCGTGGTGGTCGGGCAAGGCAGCAAAGTCGCTGCACCGCGTA
CGGCGGCGCGTGCACCGCGCGCAGCACCTGTGGCCGCGCCAGTCCGCACGTTTACGCCGACTGCCAGAGCCATTCGCGGC
GTGGATTTCCAGCGTGGCACGGCGGGCGAGGGCAATGTGGTCATCGATTTGTCCGACCCGGCCATCGCTCCGGACATCCA
GGAGCACGACGGCAAGATCATGCTCAATTTCGCCCGTACCCAATTGCCCGAACCGCTGCGGGTACGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGTGTCAGCGGTGATCGGGCGACGATCACCGTCGAACCCAGCGGTACG
TTCGAGTATTCCACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGCCCATTGACCATCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTACAGCGGCGAAAAGCTCTCGCTCAACTTTCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTGGC
GCCCGCAGATGAGATTGCAGCGCGGGAACGGCAGGAACTGGAGTCGCAGAAACAGATCGCTGAGCTGGCGCCGTTGCGCC
GCGAGCTGCTGCAAGTGAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCCAAG
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
GCTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ACTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGCTCTTCGACCACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGTACAGTCAACAACAC
GTCCGGGATCGGCATCGCATTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACCGCGATGGAGAAAACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAAGCGTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTTCCGC
CGATCAAGAAGAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.608

100

0.74
  pilQ Acinetobacter baumannii strain A118

38.051

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.051

100

0.412
  comQ Acinetobacter baylyi ADP1

40.778

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.926

97.543

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.89

97.254

0.368