Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   I5961_RS02030 Genome accession   NZ_CP065410
Coordinates   448460..449524 (+) Length   354 a.a.
NCBI ID   WP_007952389.1    Uniprot ID   J2PGW6
Organism   Pseudomonas sp. IAC-BECa141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443460..454524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5961_RS02020 (I5961_02020) - 444259..445527 (+) 1269 WP_085698113.1 malic enzyme-like NAD(P)-binding protein -
  I5961_RS02025 (I5961_02025) - 445797..448241 (-) 2445 WP_371918985.1 penicillin-binding protein 1A -
  I5961_RS02030 (I5961_02030) comM 448460..449524 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  I5961_RS02035 (I5961_02035) - 449524..450090 (+) 567 WP_085698115.1 PilN domain-containing protein -
  I5961_RS02040 (I5961_02040) pilO 450087..450710 (+) 624 WP_085698116.1 type 4a pilus biogenesis protein PilO -
  I5961_RS02045 (I5961_02045) - 450707..451231 (+) 525 WP_227234185.1 pilus assembly protein PilP -
  I5961_RS02050 (I5961_02050) pilQ 451246..453324 (+) 2079 WP_085698118.1 type IV pilus secretin PilQ Machinery gene
  I5961_RS02055 (I5961_02055) aroK 453329..453847 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38097.60 Da        Isoelectric Point: 4.6857

>NTDB_id=509226 I5961_RS02030 WP_007952389.1 448460..449524(+) (comM) [Pseudomonas sp. IAC-BECa141]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=509226 I5961_RS02030 WP_007952389.1 448460..449524(+) (comM) [Pseudomonas sp. IAC-BECa141]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGTTCCACATCGGTGAAGCTGCTGGAGCT
GAGTCGTCAGGGGGAGCGCTACCGGGTCGAGGCGTATGCGGTGGAGCCGTTGCCGGCGAGTGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCCCTGTCCCGGGTCCTGGTCAAGGCGCGCACCGGGCTGAAAACCGTGGCAGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTTATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCACTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTCTCGCCGC
GCAACCCCGAGCGGGTCAACGTACTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTCGCACTG
GCCGGGCTGACCGCGCGGGTGGTGGATGTCGAAGCCTATGCGCTGGAGCGTTCGTTCGGCCTGCTGGCCACGCAACTGGC
CGCGTCCCAGGACCGGCTGACCGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAATGGCC
GGATCATCTACACCCGCGAACAATTGTTCGGTGGTCGACAACTGACCGAGGAAATCCAGCGCCGTTATGGTCTGACCCTT
GAGCAGGCGGGGCTCGCCAAGAAGCAGGGTGGGCTGCCGGACGACTACGTCAGCGAGGTGCTGCAACCGTTTCGCGAGGC
GCTGGTGCAGCAGGTGTCGCGTTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCCGTCGACCACATACTGTTGG
CCGGCGGCACGGCGTCGGTGCCCGGGCTCGACCGGCTGATCGAAGAGCGCCTGAACACCCCGACCCAGGTTGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAAGTCAACGCCGGGGCGCTGGCAAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTTGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2PGW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.577

100

0.497