Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BTD91_RS09085 Genome accession   NZ_CP065150
Coordinates   2013718..2015403 (-) Length   561 a.a.
NCBI ID   WP_077680842.1    Uniprot ID   -
Organism   Vibrio kanaloae strain R17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2008718..2020403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTD91_RS09055 (BTD91_09055) rplS 2009278..2009631 (+) 354 WP_017056604.1 50S ribosomal protein L19 -
  BTD91_RS09060 (BTD91_09060) yacG 2009874..2010071 (-) 198 WP_077680846.1 DNA gyrase inhibitor YacG -
  BTD91_RS09065 (BTD91_09065) zapD 2010152..2010892 (-) 741 WP_029627015.1 cell division protein ZapD -
  BTD91_RS09070 (BTD91_09070) coaE 2010919..2011533 (-) 615 WP_077680845.1 dephospho-CoA kinase -
  BTD91_RS09075 (BTD91_09075) pilD 2011536..2012405 (-) 870 WP_077680844.1 prepilin peptidase Machinery gene
  BTD91_RS09080 (BTD91_09080) pilC 2012473..2013702 (-) 1230 WP_077680843.1 type II secretion system F family protein Machinery gene
  BTD91_RS09085 (BTD91_09085) pilB 2013718..2015403 (-) 1686 WP_077680842.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BTD91_RS09090 (BTD91_09090) - 2015407..2015859 (-) 453 WP_077680841.1 pilin -
  BTD91_RS09095 (BTD91_09095) nadC 2016116..2017006 (-) 891 WP_077680840.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BTD91_RS09100 (BTD91_09100) ampD 2017090..2017691 (+) 602 Protein_1690 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BTD91_RS09105 (BTD91_09105) pdhR 2018173..2018934 (+) 762 WP_017056615.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62227.03 Da        Isoelectric Point: 4.9795

>NTDB_id=507439 BTD91_RS09085 WP_077680842.1 2013718..2015403(-) (pilB) [Vibrio kanaloae strain R17]
MLTNLPTVLRQANLLSLTQEQAVAEYVQASGVSTPEALLVLDLFSGDSLANNIKTIFGLPLVQLANTDYETLCEQLGLRE
LITKYCAIPIAVSSSTLTLASADPTDLHAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSIFGQDSKRKEITQDELAN
LVEVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LSKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEEQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLRGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTVDDSPDIFLRHSIPNSQT
IYKANPQGCNECTQGYSGRVGIYEVMPFTDQLKSSLINKPNALTIESLARREGMRTLQESGLDKLLEGITSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=507439 BTD91_RS09085 WP_077680842.1 2013718..2015403(-) (pilB) [Vibrio kanaloae strain R17]
ATGCTCACTAATCTCCCAACCGTTCTTCGTCAGGCTAATTTACTTAGCCTGACTCAAGAGCAAGCAGTTGCGGAATATGT
ACAAGCTTCAGGTGTTTCTACGCCTGAAGCTTTACTCGTTCTTGATTTATTCAGCGGCGATTCCTTAGCAAACAATATCA
AAACCATTTTCGGTTTACCGTTAGTACAACTCGCCAATACCGACTACGAAACCTTGTGTGAACAGCTAGGGCTTCGCGAG
CTGATCACCAAGTATTGTGCTATTCCGATTGCCGTTTCTAGTTCTACTCTCACGCTCGCCTCTGCCGACCCGACCGACTT
ACACGCTGAAGACGATTTCAGATTTGCGACAGGGTTGCAAATTGAACTCGTTGTCGCTAATTATTCAGAATTAGAAGGCG
CGATACGCAAACTGTATGGCCGCTCGATTTTCGGACAAGACTCCAAGCGAAAAGAGATCACTCAAGATGAACTCGCCAAC
CTAGTTGAAGTCTCTGACGATGAAATCACGTCAATTGAAGATCTCAGCCAAGATGACTCCCCTGTGAGCCGTTTTATCAA
CCAAATATTGGTTGATGCGGTGCGTAAAGGCGCCTCTGATATCCACTTCGAACCTTATGAAGAAAACTATCGAGTTCGCC
TGCGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCTGCTTCTCATTTAAGTCGTCGGTTATCCGCCCGCTTAAAAATT
CTCTCTAAGTTGGATATTGCCGAGCGTCGGTTGCCTCAAGACGGCCGCATCAAGTTGCGGTTAAATGATGAGCTTGCGAT
TGATATGCGTGTATCGACGCTACCGACATTATGGGGCGAAAAGATCGTACTGCGTCTGCTAGACAGTAGTGCTGCCAATT
TAGATATCGATAAACTCGGCTACAGCGAAGAACAAAAAGCACTCTACCTTAATGCACTGAAACGACCACAAGGGATGATT
TTAATGACCGGGCCAACCGGCAGTGGCAAAACAGTCTCCCTTTACACCGGCCTTCGAGTTCTCAACACCACAGAACGAAA
CATCTCTACCGCAGAAGATCCGGTCGAAATTAACTTACGTGGTATCAACCAAGTACAAGTCACACCAAAGATCGGCTTTG
GGTTTGCTGAAGCACTACGATCCTTTTTACGGCAAGACCCAGACATCGTGATGGTCGGAGAGATCCGTGACTTAGAAACA
GCAGAGATAGCGATCAAAGCCTCGCAAACTGGCCACTTAGTTCTTTCAACACTGCATACAAACTCTGCAGCAGAAACCGT
CACTCGACTCGCTCACATGGGGATAGAGCCCTTTAACCTTGCTTCATCATTAAGCCTAATTATTGCTCAGCGACTCGCGA
GACGCTTGTGCAACCATTGCAAAACGGTGGATGATTCACCGGACATATTTCTGCGTCACTCTATTCCCAACAGCCAAACC
ATTTACAAAGCCAACCCACAAGGATGCAATGAGTGTACTCAAGGGTATTCTGGCCGAGTCGGTATCTACGAAGTGATGCC
GTTTACCGACCAACTCAAAAGCAGCCTTATCAACAAGCCTAACGCGCTAACAATTGAAAGCCTTGCTCGTCGGGAAGGTA
TGCGAACCCTACAAGAATCAGGGCTTGATAAGCTGCTTGAAGGTATCACCAGCTACCAAGAACTACAGCGTGTTCTGTAC
ATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.936

100

0.743
  pilB Vibrio campbellii strain DS40M4

70.641

100

0.708
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Legionella pneumophila strain ERS1305867

48.122

99.643

0.48
  pilB Acinetobacter baumannii D1279779

51.445

92.513

0.476
  pilB Acinetobacter baylyi ADP1

49.615

92.692

0.46
  pilF Neisseria gonorrhoeae MS11

44.262

97.861

0.433
  pilF Thermus thermophilus HB27

39.155

92.87

0.364