Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DKB71_RS02545 Genome accession   NZ_CP064949
Coordinates   552496..554541 (+) Length   681 a.a.
NCBI ID   WP_408599534.1    Uniprot ID   -
Organism   Pseudomonas sp. PLMAX     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547496..559541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKB71_RS02525 (DKB71_02510) pilM 549714..550775 (+) 1062 WP_408599530.1 pilus assembly protein PilM Machinery gene
  DKB71_RS02530 (DKB71_02515) - 550775..551341 (+) 567 WP_408599531.1 PilN domain-containing protein -
  DKB71_RS02535 (DKB71_02520) - 551338..551958 (+) 621 WP_408599532.1 type 4a pilus biogenesis protein PilO -
  DKB71_RS02540 (DKB71_02525) - 551958..552482 (+) 525 WP_408599533.1 pilus assembly protein PilP -
  DKB71_RS02545 (DKB71_02530) pilQ 552496..554541 (+) 2046 WP_408599534.1 type IV pilus secretin PilQ Machinery gene
  DKB71_RS02550 (DKB71_02535) aroK 554546..555064 (+) 519 WP_032831512.1 shikimate kinase AroK -
  DKB71_RS02555 (DKB71_02540) aroB 555215..556315 (+) 1101 WP_408599535.1 3-dehydroquinate synthase -
  DKB71_RS02560 (DKB71_02545) - 556326..557909 (+) 1584 WP_408599536.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 681 a.a.        Molecular weight: 73818.10 Da        Isoelectric Point: 5.4327

>NTDB_id=505752 DKB71_RS02545 WP_408599534.1 552496..554541(+) (pilQ) [Pseudomonas sp. PLMAX]
MNRIFSALGVSLWIALLSPMVQAATLKTLDVAALPGDRVELKLVFDEPPPAPRGYTTESPARIALDLPGVVSQLASKTRD
LGNGNARSATVVEAKDRARLIINLNQLSPYDFRVEGNNLFVVVGQGGKSPTPRTAATAVPVPAPKRAVAPAGKAIRAVDF
QRGTQGEGNVVIDLSDPSIAPDIQEREGKIILGFARTRLPEPLRVRLDVKDFATPVQFVNASASGDKATITIEPGGAFDY
STYQTDNKLTVSVRPMTVDDLQKRNADRNAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWD
QALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKADE
RGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGAIKKGNWNASGVGNGAND
SSASSPFVDLGVANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEASSSGATSV
SFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2046 bp        

>NTDB_id=505752 DKB71_RS02545 WP_408599534.1 552496..554541(+) (pilQ) [Pseudomonas sp. PLMAX]
ATGAACAGGATTTTCTCCGCCCTCGGAGTATCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCACCCTCAA
GACGCTGGATGTCGCCGCGTTGCCGGGAGATCGCGTCGAGTTGAAGCTGGTGTTCGACGAGCCACCCCCGGCGCCCCGTG
GCTACACCACGGAATCTCCCGCCAGGATTGCCCTGGATCTTCCCGGCGTGGTCAGTCAGCTGGCGAGCAAGACTCGCGAC
CTGGGCAATGGCAATGCCCGCAGCGCGACGGTGGTGGAGGCCAAGGACCGTGCGCGGCTGATCATCAATCTCAACCAGCT
GAGCCCCTATGACTTCCGGGTCGAAGGCAACAACCTCTTTGTAGTGGTCGGGCAGGGCGGCAAAAGCCCGACCCCGCGAA
CGGCCGCTACCGCCGTCCCGGTTCCGGCGCCCAAGCGCGCGGTTGCGCCGGCGGGCAAGGCCATTCGTGCGGTGGATTTC
CAGCGTGGTACCCAGGGCGAAGGCAATGTCGTGATTGATCTGTCGGACCCGTCCATTGCACCGGATATTCAGGAACGCGA
AGGCAAGATCATTCTCGGATTTGCCAGGACCCGATTGCCCGAGCCATTGCGCGTACGCCTGGACGTCAAGGATTTCGCCA
CCCCGGTTCAGTTCGTCAATGCCAGTGCCAGCGGGGATAAGGCGACGATCACCATTGAACCCGGCGGCGCCTTCGATTAC
TCAACCTATCAGACCGACAACAAGCTGACCGTCAGCGTCCGCCCGATGACGGTCGACGACCTGCAAAAGCGCAACGCCGA
CCGCAATGCCTACACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGACGTGCGCTCAGTGCTGCAACTGATCGCCG
ATTTCACCAACCTCAATCTGGTCGCCAGTGACACGGTGCAGGGGGGCATTACCCTGCGCCTGCAAAATGTACCCTGGGAC
CAGGCACTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGTGGGTAATGTGCTGCTCGTCGCGCCAGCCGA
CGAAATTGCGGCCCGTGAACGTCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGACCTGGCGCCGCTGCGTCGGGAGCTTT
TGCAGGTCAATTACGCCAAGGCGGCCGATATCGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCGAAAGCCGACGAA
CGGGGATCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGTCTCGACGAACTGCGGCG
AATCGTTGCGCAGCTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATAGTCGAAGCCAACGTCGACTACGACA
AGAGCCTTGGCGTGCGCTGGGGTGGCGCGATCAAGAAAGGCAACTGGAATGCCTCCGGTGTCGGTAACGGCGCCAACGAC
AGCAGCGCCAGCTCGCCGTTCGTCGACCTGGGGGTCGCCAACAATACGTCGGGCATCGGCATTGCCTTCATCACCGACAA
CGTCTTGCTGGACCTTGAATTGACGGCCATGGAAAAGACCGGTAACGGCGAAATCGTCTCGCAACCCAAGGTGGTCACCT
CCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCAACCTCGGTC
TCGTTCAAGGAGGCGTCGCTGTCGCTGGAGGTGACGCCGCAGATCACGCCGGACAACCGGATCATCATGGAGGTCAAGGT
CACCAAGGATGAGCCGGACTACCTGAACAAGGTCCAGGATGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTCC
TGGTCAACGATGGCGAGACCATCGTCATTGGGGGTGTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTT
CTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGACGTGGTTTCGGAGAAAAAATCCGAGCTGTTGGTGTTTCT
CACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-438)

Secretin and TonB N terminus short domain (STN)

(299-345)

Bacterial type II and III secretion system protein (Secretin)

(516-672)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.879

100

0.769
  comQ Acinetobacter baylyi ADP1

41.134

100

0.416
  pilQ Acinetobacter baumannii strain A118

37.332

100

0.407
  pilQ Acinetobacter baumannii D1279779

37.332

100

0.407
  pilQ Legionella pneumophila strain Lp02

39.137

98.678

0.386
  pilQ Legionella pneumophila strain ERS1305867

39.137

98.678

0.386