Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DKB71_RS02525 Genome accession   NZ_CP064949
Coordinates   549714..550775 (+) Length   353 a.a.
NCBI ID   WP_408599530.1    Uniprot ID   -
Organism   Pseudomonas sp. PLMAX     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544714..555775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKB71_RS02510 (DKB71_02495) rpmE 545060..545278 (+) 219 WP_008047849.1 50S ribosomal protein L31 -
  DKB71_RS02515 (DKB71_02500) - 545565..546833 (+) 1269 WP_323606487.1 malic enzyme-like NAD(P)-binding protein -
  DKB71_RS02520 (DKB71_02505) - 547058..549499 (-) 2442 WP_408599529.1 penicillin-binding protein 1A -
  DKB71_RS02525 (DKB71_02510) pilM 549714..550775 (+) 1062 WP_408599530.1 pilus assembly protein PilM Machinery gene
  DKB71_RS02530 (DKB71_02515) - 550775..551341 (+) 567 WP_408599531.1 PilN domain-containing protein -
  DKB71_RS02535 (DKB71_02520) - 551338..551958 (+) 621 WP_408599532.1 type 4a pilus biogenesis protein PilO -
  DKB71_RS02540 (DKB71_02525) - 551958..552482 (+) 525 WP_408599533.1 pilus assembly protein PilP -
  DKB71_RS02545 (DKB71_02530) pilQ 552496..554541 (+) 2046 WP_408599534.1 type IV pilus secretin PilQ Machinery gene
  DKB71_RS02550 (DKB71_02535) aroK 554546..555064 (+) 519 WP_032831512.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37749.23 Da        Isoelectric Point: 4.8803

>NTDB_id=505751 DKB71_RS02525 WP_408599530.1 549714..550775(+) (pilM) [Pseudomonas sp. PLMAX]
MPGLFNKKARSLLGIDISSTSVKLLELSRQGNGYRVEAYAVESLPPSAVIEKNIAELDGVGQALTRVLAKARTSLKGVAV
AVAGSAVITKTIEMDAGLCDDEMEDQLKIEADQYIPYPLDEVAIDFEVQGASVRNPERVNVLLAACRKENVEIREAALAL
AGLTARVVDVEAYALERSFSLLKSQGGGLQGRLVAVVDVGATMTTLSVLHDGRIIYTREQLFGGRQLTEDIQRRYGLTVE
QAGLAKKQGGLPDDYVSVVLQPFREALVQQVSRSLQFFFASGHYTAVDHILLVGGTASVSGLDQLIEQQLGTPTRVANPF
ADMTLSSKVNAAALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=505751 DKB71_RS02525 WP_408599530.1 549714..550775(+) (pilM) [Pseudomonas sp. PLMAX]
GTGCCAGGACTCTTCAATAAAAAGGCCAGGTCGCTCCTGGGCATCGATATCAGCTCGACATCGGTGAAGCTGCTGGAGCT
GAGCCGGCAGGGCAATGGCTATCGTGTCGAAGCCTACGCGGTCGAGTCGTTACCCCCCAGTGCGGTCATCGAGAAAAATA
TCGCCGAGCTCGATGGCGTGGGCCAGGCCTTGACGCGTGTGCTGGCCAAGGCTCGCACCAGTCTCAAGGGCGTTGCCGTG
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGATGCCGGCCTTTGCGACGACGAGATGGAAGACCAGCT
GAAGATCGAGGCCGATCAGTACATTCCTTATCCGCTGGACGAAGTGGCCATCGATTTCGAAGTCCAGGGCGCGTCAGTGC
GCAATCCTGAGCGGGTCAACGTGTTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAATCCGTGAGGCAGCCTTGGCCCTG
GCGGGCCTGACTGCGCGTGTGGTCGACGTCGAGGCCTATGCCCTGGAGCGATCGTTCAGCTTGCTGAAGAGCCAGGGAGG
GGGCTTGCAAGGGCGACTGGTGGCGGTGGTCGATGTTGGAGCAACCATGACCACCCTGAGCGTGCTGCATGACGGGCGGA
TCATCTACACCCGCGAGCAATTGTTTGGTGGCCGCCAGCTCACCGAGGACATTCAGCGCCGATACGGCCTGACCGTCGAG
CAGGCCGGCCTGGCGAAGAAGCAGGGCGGTTTGCCGGACGATTATGTCAGCGTTGTGTTGCAGCCGTTTCGTGAGGCCCT
GGTGCAGCAAGTGTCGCGTTCGTTGCAGTTCTTCTTTGCTTCGGGCCATTACACCGCGGTCGACCACATACTGCTGGTGG
GCGGGACCGCGTCGGTGTCCGGTCTGGACCAGTTGATCGAGCAGCAATTGGGCACGCCGACCCGGGTGGCCAATCCGTTT
GCCGACATGACCTTGAGCAGCAAGGTCAACGCCGCGGCCCTGGCCAGCGATGCGCCCGCGCTGATGATTGCCTGCGGGCT
GGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.571

99.15

0.521
  comM Acinetobacter nosocomialis M2

52.571

99.15

0.521
  comM Acinetobacter baylyi ADP1

51.558

100

0.516
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.47