Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IS481_RS03815 Genome accession   NZ_CP064338
Coordinates   782811..784529 (-) Length   572 a.a.
NCBI ID   WP_104358373.1    Uniprot ID   A0A2S5T213
Organism   Caldimonas thermodepolymerans strain DSM 15344     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 777811..789529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS481_RS03785 (IS481_03785) - 778522..778950 (+) 429 WP_104358367.1 NUDIX domain-containing protein -
  IS481_RS03790 (IS481_03790) - 778974..779174 (-) 201 WP_104358368.1 DNA gyrase inhibitor YacG -
  IS481_RS03795 (IS481_03795) zapD 779191..779946 (-) 756 WP_104358369.1 cell division protein ZapD -
  IS481_RS03800 (IS481_03800) coaE 780049..780657 (-) 609 WP_104358370.1 dephospho-CoA kinase -
  IS481_RS03805 (IS481_03805) pilD 780677..781534 (-) 858 WP_104358371.1 prepilin peptidase Machinery gene
  IS481_RS03810 (IS481_03810) pilC 781544..782764 (-) 1221 WP_104358372.1 type II secretion system F family protein Machinery gene
  IS481_RS03815 (IS481_03815) pilB 782811..784529 (-) 1719 WP_104358373.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IS481_RS03825 (IS481_03825) ispB 784848..785777 (-) 930 WP_232529566.1 octaprenyl diphosphate synthase -
  IS481_RS03830 (IS481_03830) rplU 786062..786373 (+) 312 WP_104358374.1 50S ribosomal protein L21 -
  IS481_RS03835 (IS481_03835) rpmA 786410..786667 (+) 258 WP_104358375.1 50S ribosomal protein L27 -
  IS481_RS03840 (IS481_03840) - 786778..786972 (+) 195 WP_104358376.1 LiaI-LiaF-like domain-containing protein -
  IS481_RS03845 (IS481_03845) cgtA 786969..788069 (+) 1101 WP_104358377.1 Obg family GTPase CgtA -
  IS481_RS03850 (IS481_03850) proB 788114..789232 (+) 1119 WP_104358378.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62506.68 Da        Isoelectric Point: 5.3558

>NTDB_id=500848 IS481_RS03815 WP_104358373.1 782811..784529(-) (pilB) [Caldimonas thermodepolymerans strain DSM 15344]
MDSTLAEAPNPTLSGVARVLVNAGKLNAKTAEELAKQAQEKRISFVSAVIAAGAVSASDLAHTLSNALSLPLMDLSAVDP
QRLPKGLLENKLSAQYQVVVLGKRGSRLFVGGADPTDQEALERIKFATQLTPEWVIVEHDKLTRMLEATNASASEALENI
VGGEFEFDVSEEDTQQESADVTADVEDAPVVRFLQKMLIDAINMRASDLHFEPYEYNYRVRFRVDGELREIAQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGNRPIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEKEEKERLLEAI
HRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNISTVEDPAEINLPGINQVNVNDKAGLTFAAALKAFLRQDPDIIMVG
EIRDLETADIAIKAAQTGHLVMSTLHTNDAPSTLTRLRNMGVAPFNIASSVILITAQRLARRLCENCKAPATYPREALLR
AGFKEEELDGSWKPYRAVGCSACNNGYKGRVGLYQVMPISEEIQRIILADGSSMDIAAQAQREGVRDLRQAGLLKVKLGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=500848 IS481_RS03815 WP_104358373.1 782811..784529(-) (pilB) [Caldimonas thermodepolymerans strain DSM 15344]
ATGGACAGCACACTGGCTGAGGCCCCGAATCCGACCCTGTCTGGCGTTGCGCGGGTGCTCGTGAATGCGGGCAAGCTCAA
CGCAAAGACGGCCGAGGAGCTGGCCAAACAGGCCCAGGAAAAACGCATCAGTTTCGTGTCGGCCGTGATCGCGGCAGGCG
CGGTCTCGGCCAGCGACCTGGCCCACACGCTGTCGAATGCGCTCTCGCTGCCGCTGATGGACCTGTCGGCGGTGGATCCG
CAGCGCCTGCCCAAGGGCCTGCTGGAAAACAAGCTCAGCGCGCAGTACCAGGTGGTGGTGCTGGGCAAGCGCGGCAGCCG
GCTGTTCGTCGGCGGGGCCGACCCCACCGACCAGGAAGCCCTGGAGCGCATCAAGTTCGCGACCCAGCTGACGCCCGAGT
GGGTGATCGTCGAGCACGACAAGCTGACCCGCATGCTGGAGGCGACCAATGCCTCCGCGAGCGAGGCGCTGGAGAACATC
GTCGGCGGCGAGTTCGAGTTCGACGTCTCCGAGGAGGACACCCAGCAGGAGTCGGCCGACGTCACTGCCGACGTCGAGGA
CGCGCCGGTCGTGCGCTTCCTGCAGAAGATGCTGATCGATGCGATCAACATGCGCGCCTCGGACCTGCACTTCGAGCCCT
ACGAGTACAACTACCGGGTGCGCTTCCGCGTCGACGGCGAGTTGCGCGAGATCGCCCAGCCGCCGATCGCGATCAAGGAC
AAGCTGGCCTCGCGCATCAAGGTCATCTCCCGCCTGGACATTGCCGAGAAGCGCGTGCCGCAGGACGGCCGGATGAAGCT
GAAGTTCGGCAACCGGCCGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATCCGTATCC
TGGATCCGTCCAGTGCCAAGCTGGGCATCGACGCGCTCGGCTACGAGAAGGAAGAGAAGGAACGCTTGCTGGAGGCGATC
CACCGCCCCTACGGGATGATCCTCGTGACCGGCCCGACCGGCAGCGGCAAGACCGTGTCGCTGTACACCTGCCTGAACCT
GCTGAACCAGCCGGGCGTGAACATCTCCACCGTGGAAGACCCGGCGGAAATCAACCTGCCCGGGATCAACCAGGTCAACG
TCAACGACAAGGCGGGCCTGACCTTCGCGGCCGCGCTGAAGGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGC
GAGATCCGGGACCTGGAAACCGCCGACATCGCGATCAAGGCCGCCCAGACCGGTCACCTGGTGATGTCGACGCTGCACAC
CAACGACGCACCGTCGACACTGACGCGTCTGCGCAACATGGGGGTGGCCCCCTTCAACATCGCGTCCAGCGTGATCCTGA
TCACGGCGCAACGGCTCGCGCGGCGCCTGTGCGAGAACTGCAAGGCCCCCGCAACGTATCCTCGCGAAGCGCTGTTGCGC
GCCGGCTTCAAGGAAGAGGAACTGGACGGCAGCTGGAAGCCCTACCGGGCGGTCGGTTGCTCGGCCTGCAACAACGGCTA
CAAGGGGCGGGTCGGCCTGTACCAGGTCATGCCGATCTCGGAGGAGATCCAGCGCATCATCCTGGCCGACGGCAGTTCGA
TGGACATCGCGGCGCAAGCCCAGAGGGAGGGCGTGCGCGACCTGCGACAGGCCGGGCTGCTGAAGGTCAAGCTGGGCGTG
ACGACGCTGGAAGAAGTGATTTCCGTCACGAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)

Type II/IV secretion system protein (T2SSE)

(196-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S5T213

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.77

98.951

0.542
  pilB Acinetobacter baylyi ADP1

54.69

98.776

0.54
  pilF Neisseria gonorrhoeae MS11

54.804

98.252

0.538
  pilB Legionella pneumophila strain ERS1305867

50.709

98.601

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

46.316

99.65

0.462
  pilB Vibrio cholerae strain A1552

46.429

97.902

0.455
  pilB Vibrio campbellii strain DS40M4

45.455

98.077

0.446
  pilF Thermus thermophilus HB27

37.986

98.951

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

92.133

0.367