Detailed information NTG0500847

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: JKN65_RS01435
• Genome accession: NZ_CP076241
• Coordinates: 289402..290385 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain Vac_07.1
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 284402..295385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JKN65_RS01420 (JKN65_01395)	dppA	285702..287309 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
JKN65_RS01425 (JKN65_01400)	dppB	287460..288479 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
JKN65_RS01430 (JKN65_01405)	dppC	288489..289391 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
JKN65_RS01435 (JKN65_01410)	amiE	289402..290385 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
JKN65_RS01440 (JKN65_01415)	dppF	290382..291386 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
JKN65_RS01445 (JKN65_01420)	yhjV	291416..292687 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
JKN65_RS01450 (JKN65_01425)	ldrD	293163..293270 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
JKN65_RS01455 (JKN65_01430)	bcsG	293357..295036 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
JKN65_RS01460 (JKN65_01435)	bcsF	295033..295224 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=500847 JKN65_RS01435 WP_001196486.1 289402..290385(+) (amiE) [Escherichia coli O157:H7 strain Vac_07.1]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=500847 JKN65_RS01435 WP_001196486.1 289402..290385(+) (amiE) [Escherichia coli O157:H7 strain Vac_07.1]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404