Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IS481_RS03810 Genome accession   NZ_CP064338
Coordinates   781544..782764 (-) Length   406 a.a.
NCBI ID   WP_104358372.1    Uniprot ID   A0A2S5T239
Organism   Caldimonas thermodepolymerans strain DSM 15344     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 776544..787764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS481_RS03775 (IS481_03775) pdeM 776945..777598 (+) 654 WP_104358365.1 ligase-associated DNA damage response endonuclease PdeM -
  IS481_RS03780 (IS481_03780) - 777647..778525 (+) 879 WP_104358366.1 ATP-binding protein -
  IS481_RS03785 (IS481_03785) - 778522..778950 (+) 429 WP_104358367.1 NUDIX domain-containing protein -
  IS481_RS03790 (IS481_03790) - 778974..779174 (-) 201 WP_104358368.1 DNA gyrase inhibitor YacG -
  IS481_RS03795 (IS481_03795) zapD 779191..779946 (-) 756 WP_104358369.1 cell division protein ZapD -
  IS481_RS03800 (IS481_03800) coaE 780049..780657 (-) 609 WP_104358370.1 dephospho-CoA kinase -
  IS481_RS03805 (IS481_03805) pilD 780677..781534 (-) 858 WP_104358371.1 prepilin peptidase Machinery gene
  IS481_RS03810 (IS481_03810) pilC 781544..782764 (-) 1221 WP_104358372.1 type II secretion system F family protein Machinery gene
  IS481_RS03815 (IS481_03815) pilB 782811..784529 (-) 1719 WP_104358373.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IS481_RS03825 (IS481_03825) ispB 784848..785777 (-) 930 WP_232529566.1 octaprenyl diphosphate synthase -
  IS481_RS03830 (IS481_03830) rplU 786062..786373 (+) 312 WP_104358374.1 50S ribosomal protein L21 -
  IS481_RS03835 (IS481_03835) rpmA 786410..786667 (+) 258 WP_104358375.1 50S ribosomal protein L27 -
  IS481_RS03840 (IS481_03840) - 786778..786972 (+) 195 WP_104358376.1 LiaI-LiaF-like domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44319.16 Da        Isoelectric Point: 9.4891

>NTDB_id=500847 IS481_RS03810 WP_104358372.1 781544..782764(-) (pilC) [Caldimonas thermodepolymerans strain DSM 15344]
MATAAAAKKIQDYIFEWEGKDRNGKPVRGEMRAGGEAMVSASLRRQGVLVTKVKKRRTSGGKAIKQKDIAIFTRQLATMM
RAGVPLLQSFDIVARGSTNPRMTRLLNEIRADVETGTSLSAAFRKHPLYFDALYCNLVEAGEAGGILETLLERLATYQEK
TLAIKAKIKSALIYPVAVLVVAFVVVALIMIFVIPAFKDIFSSFGADLPAPTLLVIAMSEFFVEYWWAIFGVVIGGTYFF
FESWKRSEKMQKTMDRLLLKVPVFGDLVYKSAVARWTRTLATMFAAGVPLVEALDSVGGASGNAVFAEATEKIQKDVATG
SSLTMSMTTTGLFPVMVLQMCSIGEESGSLDHMLNKAAEFYEQEVDEAVKGLSSLMEPIIIVVLGTLIGGIVVSMYLPIF
KLGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=500847 IS481_RS03810 WP_104358372.1 781544..782764(-) (pilC) [Caldimonas thermodepolymerans strain DSM 15344]
ATGGCAACTGCTGCTGCGGCGAAAAAGATTCAGGACTACATCTTCGAGTGGGAGGGCAAGGACCGCAATGGCAAGCCGGT
GCGCGGCGAAATGCGCGCCGGCGGGGAAGCGATGGTCAGCGCCAGCCTGCGCCGGCAGGGCGTGCTGGTCACCAAGGTCA
AGAAGCGCCGCACCAGCGGCGGCAAGGCCATCAAGCAGAAGGACATCGCCATCTTCACCCGGCAGCTGGCGACCATGATG
CGCGCCGGCGTGCCCCTGCTGCAGTCCTTCGACATCGTCGCCCGCGGCAGCACCAACCCGCGCATGACGCGGCTGCTCAA
CGAGATCCGCGCCGACGTCGAGACCGGCACCAGCCTGTCGGCCGCCTTCCGCAAGCACCCGCTGTACTTCGACGCGCTGT
ACTGCAACCTGGTCGAGGCCGGTGAGGCCGGCGGTATCCTCGAGACCCTGCTGGAGCGCCTGGCGACCTACCAGGAAAAG
ACGCTGGCGATCAAGGCCAAGATCAAGTCCGCCCTGATCTACCCGGTGGCGGTGCTGGTGGTGGCCTTCGTGGTCGTCGC
GCTGATCATGATCTTCGTGATTCCCGCGTTCAAGGACATCTTCTCGTCCTTCGGCGCGGACCTGCCCGCCCCGACGCTGC
TGGTCATCGCGATGTCGGAGTTCTTCGTCGAGTACTGGTGGGCCATCTTCGGCGTCGTCATCGGCGGCACCTACTTCTTC
TTCGAGTCGTGGAAGCGCTCGGAGAAGATGCAGAAGACGATGGACCGCCTGCTGCTGAAGGTGCCGGTGTTCGGCGACCT
GGTCTACAAGTCGGCGGTCGCGCGCTGGACCCGCACGCTGGCCACCATGTTCGCCGCAGGCGTGCCGCTGGTCGAGGCGC
TCGACTCGGTCGGCGGGGCTTCGGGCAACGCGGTGTTCGCCGAAGCCACCGAGAAGATCCAGAAGGACGTCGCGACAGGC
TCCAGCCTGACCATGTCGATGACCACCACCGGCCTGTTCCCGGTGATGGTGCTGCAGATGTGTTCGATCGGCGAGGAATC
GGGTTCGCTGGACCACATGCTGAACAAGGCGGCCGAGTTCTACGAGCAGGAAGTCGACGAGGCGGTCAAGGGCCTGTCCA
GCCTGATGGAGCCGATCATCATCGTCGTGCTCGGCACGCTGATCGGTGGCATCGTGGTCTCGATGTACCTGCCCATCTTC
AAGCTCGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S5T239

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.198

99.507

0.549
  pilG Neisseria meningitidis 44/76-A

54

98.522

0.532
  pilG Neisseria gonorrhoeae MS11

54

98.522

0.532
  pilC Legionella pneumophila strain ERS1305867

51.768

97.537

0.505
  pilC Acinetobacter baylyi ADP1

49.631

100

0.498
  pilC Acinetobacter baumannii D1279779

50.126

97.783

0.49
  pilC Vibrio cholerae strain A1552

42.677

97.537

0.416
  pilC Vibrio campbellii strain DS40M4

40.342

100

0.406
  pilC Thermus thermophilus HB27

37.965

99.261

0.377