Detailed information NTG0500846

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IS481_RS03805
• Genome accession: NZ_CP064338
• Coordinates: 780677..781534 (-)
• Length: 285 a.a.
• NCBI ID: WP_104358371.1
• Uniprot ID: A0A2S5T1W5
• Organism: Caldimonas thermodepolymerans strain DSM 15344
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 775677..786534

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IS481_RS03770 (IS481_03770)	slmA	776193..776864 (+)	672	WP_104358413.1	nucleoid occlusion factor SlmA	-
IS481_RS03775 (IS481_03775)	pdeM	776945..777598 (+)	654	WP_104358365.1	ligase-associated DNA damage response endonuclease PdeM	-
IS481_RS03780 (IS481_03780)	-	777647..778525 (+)	879	WP_104358366.1	ATP-binding protein	-
IS481_RS03785 (IS481_03785)	-	778522..778950 (+)	429	WP_104358367.1	NUDIX domain-containing protein	-
IS481_RS03790 (IS481_03790)	-	778974..779174 (-)	201	WP_104358368.1	DNA gyrase inhibitor YacG	-
IS481_RS03795 (IS481_03795)	zapD	779191..779946 (-)	756	WP_104358369.1	cell division protein ZapD	-
IS481_RS03800 (IS481_03800)	coaE	780049..780657 (-)	609	WP_104358370.1	dephospho-CoA kinase	-
IS481_RS03805 (IS481_03805)	pilD	780677..781534 (-)	858	WP_104358371.1	prepilin peptidase	Machinery gene
IS481_RS03810 (IS481_03810)	pilC	781544..782764 (-)	1221	WP_104358372.1	type II secretion system F family protein	Machinery gene
IS481_RS03815 (IS481_03815)	pilB	782811..784529 (-)	1719	WP_104358373.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IS481_RS03825 (IS481_03825)	ispB	784848..785777 (-)	930	WP_232529566.1	octaprenyl diphosphate synthase	-
IS481_RS03830 (IS481_03830)	rplU	786062..786373 (+)	312	WP_104358374.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 285 a.a.
• Molecular weight: 30818.55 Da
• Isoelectric Point: 7.9590

>NTDB_id=500846 IS481_RS03805 WP_104358371.1 780677..781534(-) (pilD) [Caldimonas thermodepolymerans strain DSM 15344]
MNNELWGWWLSPAVLGVLGACIGSFLNVVIHRLPLMLERQWKRDSAEMLGQPVPEDAGEPLSLSRPRSRCPHCGHQIRWH
ENIPVLGWLMLKGRCSSCQARISPRYPLVELLTAALFAATAWKLGPQPVALLWCAAIAVLVALTFIDWDTTLLPDDLTLP
LLWAGLVVAGLGWTIPLSSALWGAVGGYLSLWGVYWLFKLTTGKEGMGYGDFKLLAALGAWLGPAMLLPILVGASVIGTV
VGVVMKASGALREGRYVPFGPFLAGAGIAVFLIGPDAVLAWLGWY

Nucleotide

• Length: 858 bp

>NTDB_id=500846 IS481_RS03805 WP_104358371.1 780677..781534(-) (pilD) [Caldimonas thermodepolymerans strain DSM 15344]
ATGAACAACGAACTTTGGGGCTGGTGGCTGTCACCGGCCGTCCTGGGCGTGCTGGGCGCCTGCATCGGCAGTTTCCTCAA
CGTCGTGATCCACCGCCTGCCGCTCATGCTCGAGCGGCAGTGGAAGCGTGACAGCGCCGAGATGCTCGGCCAGCCCGTGC
CCGAAGATGCCGGCGAGCCGCTGAGCCTGTCGCGCCCGCGCTCGCGCTGCCCGCACTGCGGGCACCAGATCCGCTGGCAC
GAGAACATCCCGGTGCTCGGCTGGCTGATGCTCAAGGGCCGCTGCTCGAGCTGCCAGGCGCGCATCTCGCCCCGCTACCC
GCTGGTCGAGCTGCTGACGGCCGCGCTGTTCGCCGCCACGGCCTGGAAGCTCGGGCCGCAACCCGTCGCGCTGCTGTGGT
GCGCCGCGATCGCGGTGCTGGTGGCGCTGACCTTCATCGACTGGGACACCACCCTGCTGCCCGACGACCTGACGCTGCCG
CTGCTGTGGGCCGGCCTGGTCGTCGCCGGCCTGGGCTGGACCATCCCGCTGTCGTCGGCCCTGTGGGGCGCGGTGGGGGG
CTACCTCAGCCTCTGGGGCGTGTACTGGCTGTTCAAGCTGACCACCGGCAAGGAAGGCATGGGTTACGGCGACTTCAAGC
TGCTCGCCGCGCTGGGCGCCTGGCTCGGCCCGGCCATGCTGCTGCCCATCCTGGTCGGCGCCTCGGTGATCGGCACCGTG
GTCGGCGTCGTGATGAAGGCCAGCGGCGCCCTGCGCGAGGGGCGCTACGTGCCCTTCGGCCCGTTCCTCGCCGGCGCCGG
CATCGCGGTGTTCCTGATCGGCCCGGACGCCGTGCTCGCCTGGCTGGGCTGGTACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-123)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

3D structure

• AlphaFold DB: A0A2S5T1W5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.174	96.842	0.505
pilD	Vibrio campbellii strain DS40M4	50	91.228	0.456
pilD	Neisseria gonorrhoeae MS11	48.047	89.825	0.432
pilD	Acinetobacter nosocomialis M2	45.725	94.386	0.432
pilD	Acinetobacter baumannii D1279779	45.725	94.386	0.432