Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ISN74_RS03140 Genome accession   NZ_CP064030
Coordinates   760056..761783 (-) Length   575 a.a.
NCBI ID   WP_188797317.1    Uniprot ID   -
Organism   Dyella caseinilytica strain DHOB09     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 755056..766783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN74_RS03120 (ISN74_03115) - 755223..756974 (-) 1752 WP_188797311.1 glutamine--tRNA ligase/YqeY domain fusion protein -
  ISN74_RS03125 (ISN74_03120) coaE 757099..757707 (-) 609 WP_188799477.1 dephospho-CoA kinase -
  ISN74_RS03130 (ISN74_03125) pilD 757716..758573 (-) 858 WP_188797313.1 A24 family peptidase Machinery gene
  ISN74_RS03135 (ISN74_03130) pilC 758651..759919 (-) 1269 WP_188797315.1 type II secretion system F family protein Machinery gene
  ISN74_RS03140 (ISN74_03135) pilB 760056..761783 (-) 1728 WP_188797317.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISN74_RS03145 (ISN74_03140) - 762050..762904 (+) 855 WP_188799478.1 glycosyltransferase family 2 protein -
  ISN74_RS03150 (ISN74_03145) - 762897..764057 (+) 1161 WP_188797318.1 glycosyltransferase family 4 protein -
  ISN74_RS03155 (ISN74_03150) - 764050..764826 (+) 777 WP_188797321.1 class I SAM-dependent methyltransferase -
  ISN74_RS03160 (ISN74_03155) - 764839..765936 (+) 1098 WP_188797323.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62595.26 Da        Isoelectric Point: 6.8851

>NTDB_id=498521 ISN74_RS03140 WP_188797317.1 760056..761783(-) (pilB) [Dyella caseinilytica strain DHOB09]
MSSQPQSTTLGLSGMARRLVAEGLLPEADVRKAVAESNQQKKTLTSWLLDHGLVDNIRLAQIASAEFGMPMMDVTALAPA
SMPLSLISQELISKHQALPLLKRGKRLFVGIADPMQLHALDEIKFHSNCMVEPILVERTQLTRAIENAVSTLNNATPDLG
GGDLEDLALESGNGDDEGAGGGIDANANDDAPVVKFVNKILIDAIRRGASDIHFEPFETLYRVRLRMDGMLRAVASPPIK
LANRISSRLKVMAGLDIAERRVPQDGRIKLNLSKSHAMDFRVSTLPTLFGEKIVLRILDSSSAKLGIDKLGYETDQKELY
LDAIHKPYGMVLVTGPTGSGKTVSLYTALNILNTAERNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLIIAQRLARRLHGCKKQITLPPHV
LLAAGFTQQDIDAGLTIYEANGCPECNEGYKGRVGIYQVMPMVEEVQKIVLEGGNALQIAETARRIGINDLRASALLKVK
QGVTSLTEIDRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=498521 ISN74_RS03140 WP_188797317.1 760056..761783(-) (pilB) [Dyella caseinilytica strain DHOB09]
ATGTCATCGCAACCACAGTCCACCACGCTAGGCCTGTCCGGCATGGCTCGCCGCTTGGTGGCCGAAGGCTTGTTGCCAGA
GGCGGATGTTCGTAAAGCCGTTGCAGAATCGAACCAGCAGAAAAAAACGCTGACGTCATGGCTGCTGGATCATGGCTTGG
TGGACAACATTCGCCTCGCTCAAATCGCGTCCGCCGAATTCGGCATGCCGATGATGGACGTGACAGCGCTTGCACCTGCC
AGCATGCCATTGAGCCTGATCAGCCAGGAACTGATCAGCAAGCACCAGGCACTGCCGCTGCTCAAGCGCGGTAAGCGGCT
GTTTGTCGGGATCGCCGATCCGATGCAATTGCATGCCCTGGATGAGATCAAATTCCACTCCAACTGCATGGTGGAACCGA
TCCTGGTCGAGCGTACCCAGCTCACCCGTGCCATCGAAAACGCCGTCAGCACGCTGAACAACGCGACGCCGGACCTGGGC
GGAGGCGACCTGGAAGATCTTGCGCTTGAAAGCGGGAACGGTGACGATGAGGGCGCAGGCGGCGGGATTGATGCCAACGC
CAACGACGACGCCCCGGTCGTGAAGTTCGTCAACAAGATTTTGATAGACGCCATACGACGCGGCGCGTCCGACATCCACT
TCGAACCGTTCGAAACCCTGTACCGCGTTCGTTTGCGCATGGATGGCATGCTGCGCGCCGTGGCCAGCCCGCCGATAAAG
CTTGCCAACCGCATCTCATCACGCCTCAAGGTTATGGCCGGCCTGGACATTGCCGAACGGCGCGTGCCGCAGGACGGACG
CATCAAATTGAACCTGTCCAAGAGCCATGCGATGGACTTCCGCGTCAGCACGCTGCCGACGCTGTTTGGCGAAAAGATAG
TGCTGCGTATTCTGGACAGCTCATCAGCCAAGCTGGGTATCGACAAGCTCGGTTACGAAACGGACCAAAAAGAGCTTTAC
CTCGACGCCATCCACAAACCTTACGGCATGGTACTGGTCACCGGCCCCACCGGCTCGGGTAAAACGGTGTCGCTGTATAC
CGCGCTTAACATCCTCAATACTGCCGAACGCAATATCTCCACGGTGGAAGACCCGGTCGAAATCCGCGTAGAGGGCATCA
ACCAGGTCCAGCAAAACGTGAAGCGGGGCATGACTTTCGCGGCCGCCCTGCGTTCGTTCCTACGCCAAGATCCGGACGTG
ATCATGGTGGGCGAAATTCGTGACCTGGAAACAGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTC
CACCCTGCATACGAATGACGCCCCTCAGACCATTTCCCGTCTGATGAACATGGGCATCGCACCGTACAACATCACCTCGT
CGGTCACCCTGATCATCGCCCAGCGCTTGGCACGGCGCCTGCACGGCTGCAAGAAGCAGATAACGCTGCCACCCCATGTG
CTGCTGGCCGCGGGATTCACACAGCAAGATATTGATGCAGGACTGACCATTTACGAAGCAAATGGCTGCCCCGAATGCAA
CGAGGGTTATAAGGGGCGGGTGGGTATCTACCAGGTCATGCCAATGGTCGAAGAGGTCCAGAAGATCGTGCTGGAAGGTG
GAAATGCCCTGCAGATCGCCGAAACTGCTCGCAGAATAGGTATCAATGACCTGCGTGCCTCGGCGTTGCTCAAGGTCAAG
CAGGGAGTCACCAGCCTGACCGAAATCGACCGCGTGACTAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.766

100

0.55
  pilB Acinetobacter baylyi ADP1

54.72

99.478

0.544
  pilB Legionella pneumophila strain ERS1305867

50.438

99.304

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

48.845

97.913

0.478
  pilB Vibrio cholerae strain A1552

50.94

92.522

0.471
  pilF Neisseria gonorrhoeae MS11

47.173

98.435

0.464
  pilB Vibrio campbellii strain DS40M4

49.906

92.696

0.463
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.267

96.348

0.369
  pilF Thermus thermophilus HB27

37.746

97.217

0.367