Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   IRE31_RS04570 Genome accession   NZ_CP063829
Coordinates   890728..891576 (+) Length   282 a.a.
NCBI ID   WP_000705301.1    Uniprot ID   A0A0D6J8J8
Organism   Streptococcus pneumoniae strain 19A-ST320_99-176     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 885728..896576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRE31_RS04545 (IRE31_04510) - 886443..887150 (+) 708 WP_000638502.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IRE31_RS04550 (IRE31_04515) - 887155..888177 (+) 1023 WP_000609885.1 ribitol-5-phosphate dehydrogenase -
  IRE31_RS04555 (IRE31_04520) licA 888194..889063 (+) 870 WP_000411215.1 choline kinase LicA -
  IRE31_RS04560 (IRE31_04525) - 889053..889931 (+) 879 WP_000837618.1 DMT family transporter -
  IRE31_RS04565 (IRE31_04530) - 889943..890632 (+) 690 WP_000643970.1 sugar phosphate nucleotidyltransferase -
  IRE31_RS04570 (IRE31_04535) dprA/cilB/dalA 890728..891576 (+) 849 WP_000705301.1 DNA-processing protein DprA Machinery gene
  IRE31_RS04575 - 891759..891881 (-) 123 WP_001814242.1 hypothetical protein -
  IRE31_RS04580 (IRE31_04540) - 892458..893501 (+) 1044 WP_001812883.1 TIGR00341 family protein -
  IRE31_RS04585 (IRE31_04545) topA 893668..895755 (+) 2088 WP_000179644.1 type I DNA topoisomerase -
  IRE31_RS04590 (IRE31_04550) - 895861..896220 (+) 360 WP_001237223.1 YbaN family protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31034.67 Da        Isoelectric Point: 5.4047

>NTDB_id=496898 IRE31_RS04570 WP_000705301.1 890728..891576(+) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 19A-ST320_99-176]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=496898 IRE31_RS04570 WP_000705301.1 890728..891576(+) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 19A-ST320_99-176]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGCTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACCTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6J8J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae D39

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae R6

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

99.645

100

0.996
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.227

100

0.982
  dprA/cilB/dalA Streptococcus mitis SK321

97.518

100

0.975
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.357

99.291

0.55
  dprA Staphylococcus aureus N315

37.456

100

0.376
  dprA Haemophilus influenzae Rd KW20

38.202

94.681

0.362