Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IM698_RS06735 Genome accession   NZ_CP063682
Coordinates   1481223..1482908 (-) Length   561 a.a.
NCBI ID   WP_260810991.1    Uniprot ID   -
Organism   Vibrio splendidus strain ED144     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1476223..1487908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM698_RS06705 (IM698_06690) rplS 1476665..1477018 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  IM698_RS06710 (IM698_06695) yacG 1477379..1477576 (-) 198 WP_004735515.1 DNA gyrase inhibitor YacG -
  IM698_RS06715 (IM698_06700) zapD 1477658..1478398 (-) 741 WP_248372797.1 cell division protein ZapD -
  IM698_RS06720 (IM698_06705) coaE 1478425..1479039 (-) 615 WP_048612337.1 dephospho-CoA kinase -
  IM698_RS06725 (IM698_06710) pilD 1479042..1479911 (-) 870 WP_016799246.1 prepilin peptidase Machinery gene
  IM698_RS06730 (IM698_06715) pilC 1479978..1481207 (-) 1230 WP_260810990.1 type II secretion system F family protein Machinery gene
  IM698_RS06735 (IM698_06720) pilB 1481223..1482908 (-) 1686 WP_260810991.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IM698_RS06740 (IM698_06725) - 1482915..1483370 (-) 456 WP_017071017.1 pilin -
  IM698_RS06745 (IM698_06730) nadC 1483625..1484515 (-) 891 WP_260810992.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IM698_RS06750 (IM698_06735) ampD 1484599..1485198 (+) 600 WP_260810993.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IM698_RS06755 (IM698_06740) pdhR 1485681..1486442 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62057.77 Da        Isoelectric Point: 5.4038

>NTDB_id=496219 IM698_RS06735 WP_260810991.1 1481223..1482908(-) (pilB) [Vibrio splendidus strain ED144]
MLTNLPTILRQAGFLSLTQEQAVAEHVHASGISTPEALLALDFFSGDSLANNIKTIFGLPLVQLSNVDYEVLCDQLGLRE
LITKYHAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=496219 IM698_RS06735 WP_260810991.1 1481223..1482908(-) (pilB) [Vibrio splendidus strain ED144]
ATGCTAACCAACCTCCCAACCATTCTTCGTCAAGCAGGATTCCTTAGCCTGACTCAAGAACAAGCAGTTGCGGAACATGT
TCATGCTTCAGGTATATCTACACCTGAAGCTTTACTCGCTCTGGATTTCTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATATTCGGCTTACCGTTAGTACAACTTTCCAATGTTGATTATGAAGTATTATGTGACCAATTAGGGCTTCGCGAA
CTGATTACTAAATATCATGCTATCCCGATTTCAGTTTCTAGCTCCACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAAGCTGAAGATGACTTTCGATTCGCAACCGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGAAAGCTGTATGGCCGTTCTATTTCTGGGCAAGATTCCAAGCGGAAAGAGATCACCCAAGATGAGTTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATTCTGGTTGATGCGGTGCGTAAAGGTGCTTCTGATATTCACTTCGAACCCTACGAAGAACACTACCGAGTGCGCC
TACGTTGCGATGGTATATTGGTTGAAATCCAACAGCCCGCCTCTCATTTAAGCCGTCGCCTGTCAGCTCGCTTAAAGATT
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGTCGTATTAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGTGTATCTACGCTGCCAACATTGTGGGGAGAAAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATTGATAAGCTCGGTTACAGCGAGGCTCAGAAAGCCCTCTACCTTAACGCCTTGAAGCGCCCTCAAGGAATGATC
TTAATGACAGGGCCAACTGGCAGCGGTAAGACGGTTTCTCTTTATACTGGGCTTCGAGTGCTCAACACCTCTGAACGCAA
CATCTCTACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGCATCAATCAGGTTCAAGTGACACCTAAGATCGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGATCCTGATGTGGTAATGGTCGGAGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACAGGTCACTTAGTGCTTTCTACACTGCACACCAATTCTGCTGCTGAAACCGT
AACTCGGCTTGCTCACATGGGAATTGAACCCTTTAATCTTGCTTCGTCTTTAAGCTTGATTATTGCTCAGCGTCTTGCGA
GGCGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATATACAAAGCCAACTCTCAAGGGTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCAAATGCGCTAGCGATTGAAGATCTCGCGCGTCGAGAAGGCA
TGCGCACTCTGCAAGAATCAGGTCTGGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAGCTGCAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.291

100

0.747
  pilB Vibrio parahaemolyticus RIMD 2210633

70.819

100

0.709
  pilB Vibrio campbellii strain DS40M4

70.641

100

0.708
  pilB Legionella pneumophila strain ERS1305867

47.826

98.396

0.471
  pilB Acinetobacter baumannii D1279779

50.289

92.513

0.465
  pilB Acinetobacter baylyi ADP1

49.031

91.979

0.451
  pilF Neisseria gonorrhoeae MS11

43.964

98.93

0.435
  pilF Thermus thermophilus HB27

38.772

92.87

0.36