Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INQ43_RS09915 Genome accession   NZ_CP063658
Coordinates   2183726..2185456 (+) Length   576 a.a.
NCBI ID   WP_194036329.1    Uniprot ID   A0A7S6ZW93
Organism   Lysobacter sp. H23M47     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2178726..2190456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ43_RS09885 (INQ43_09885) - 2179632..2180540 (+) 909 WP_228062478.1 peptidoglycan DD-metalloendopeptidase family protein -
  INQ43_RS09890 (INQ43_09890) - 2180691..2181005 (-) 315 WP_194036324.1 type II toxin-antitoxin system RelE/ParE family toxin -
  INQ43_RS09895 (INQ43_09895) - 2180993..2181250 (-) 258 WP_194036325.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
  INQ43_RS09900 (INQ43_09900) - 2181618..2182049 (+) 432 WP_194036326.1 pilin -
  INQ43_RS09905 (INQ43_09905) - 2182597..2183040 (+) 444 WP_194036327.1 hypothetical protein -
  INQ43_RS09910 (INQ43_09910) nadS 2183151..2183441 (+) 291 WP_194036328.1 NadS family protein -
  INQ43_RS09915 (INQ43_09915) pilB 2183726..2185456 (+) 1731 WP_194036329.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INQ43_RS09920 (INQ43_09920) - 2185553..2185855 (+) 303 WP_228062479.1 helix-turn-helix transcriptional regulator -
  INQ43_RS09925 (INQ43_09925) - 2185852..2187081 (+) 1230 WP_194036331.1 type II toxin-antitoxin system HipA family toxin -
  INQ43_RS09930 (INQ43_09930) pilC 2187303..2188562 (+) 1260 WP_194036332.1 type II secretion system F family protein Machinery gene
  INQ43_RS09935 (INQ43_09935) pilD 2188576..2189439 (+) 864 WP_193984256.1 A24 family peptidase Machinery gene
  INQ43_RS09940 (INQ43_09940) coaE 2189522..2190142 (+) 621 WP_194036333.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62851.22 Da        Isoelectric Point: 5.7587

>NTDB_id=496169 INQ43_RS09915 WP_194036329.1 2183726..2185456(+) (pilB) [Lysobacter sp. H23M47]
MNAVATANLVGITGIARRLVQDGALSESAAREAMAAATELRKPLASYIIEKRLVAPAHLAAANSIEFGVPIFDAAAMDPE
QSAIKLVKEELLRKHTVLPLFKRGNRLFVGIAEPTNTHALAEIKFQTNFTVEAILVDEESIKRHLDRWLESADALGDVMG
EDDEGLENLDVGGGDDDLSGDSGVDAKTDDTPVVKFINKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQSAKVPVK
LQPRISARLKVMAQLDIAERRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIEKLGYEDDQRDIF
LAAVKRPYGMVLVTGPTGSGKTVSLYTALNILNDETRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTLVIAQRLARRLHSCKRPVELPEHA
LLAEGFTAEEIASEDFTIFEAVGCADCTEGYKGRTGIYQVMPMTDEIQGIVLAGGNAMQIAEAAQKSGVRDLRQSALMKV
RNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=496169 INQ43_RS09915 WP_194036329.1 2183726..2185456(+) (pilB) [Lysobacter sp. H23M47]
ATGAACGCCGTAGCCACTGCCAACCTCGTCGGTATCACGGGCATTGCCCGCCGCCTCGTGCAGGATGGCGCGCTTTCCGA
GTCCGCCGCGCGCGAGGCGATGGCAGCGGCTACCGAGCTGCGCAAGCCGCTGGCCAGCTACATCATCGAGAAGCGCCTTG
TCGCGCCCGCGCATCTGGCCGCGGCCAACTCCATCGAGTTCGGTGTGCCGATCTTCGATGCCGCGGCGATGGACCCCGAG
CAATCGGCCATCAAGCTGGTCAAGGAGGAACTGCTGCGCAAGCACACGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTCGGCATCGCGGAGCCGACCAACACCCACGCGCTGGCGGAGATCAAGTTCCAGACCAACTTCACCGTCGAGGCGA
TCCTGGTCGATGAGGAGAGCATCAAGCGCCATCTGGATCGCTGGCTGGAAAGCGCCGACGCGCTCGGCGATGTCATGGGC
GAGGATGACGAGGGCCTGGAGAACCTGGATGTCGGTGGCGGTGACGACGACCTGTCCGGCGATTCCGGTGTGGACGCCAA
GACCGACGACACGCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTGGACGCGATCCGCCGGGGCGCCTCCGACATCCACT
TCGAGCCCTACGAGACCGAGTACCGCGTGCGCCTGCGCATCGACGGGCTGCTGAAACAGTCCGCCAAGGTGCCCGTCAAA
TTGCAGCCGCGCATATCCGCACGCCTCAAGGTGATGGCGCAGCTGGACATTGCCGAGCGCCGGGTGCCGCAGGACGGGCG
AATCAAACTCAACCTGAGCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTCGGCTACGAGGACGACCAGCGCGATATCTTC
CTCGCCGCGGTCAAGCGCCCTTACGGCATGGTGCTGGTCACCGGCCCGACCGGGTCCGGCAAGACGGTCTCGCTGTACAC
CGCGCTGAACATCCTCAATGACGAGACCCGCAACATCTCCACCGTCGAGGATCCGGTCGAGATCCGCGTGCCCGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCCGACGTG
ATCATGGTCGGCGAGATCCGCGACCTGGAAACCGCCGAGATCGGCATCAAGGCCGCGCAGACCGGTCACATGGTGCTGTC
CACCCTGCATACCAACGACGCCCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCTCCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGTCTGCACAGCTGCAAACGCCCGGTGGAACTGCCTGAGCACGCG
CTGCTGGCGGAGGGCTTTACCGCCGAGGAAATCGCCAGCGAGGACTTCACGATTTTCGAGGCGGTCGGCTGCGCGGACTG
CACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCCAGGGCATCGTGCTGGCCG
GCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGAAGTCCGGCGTGCGCGACCTGCGCCAGTCGGCGCTGATGAAGGTG
CGCAACGGCGTCACCAGCCTGGCGGAGATCAACCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6ZW93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.073

100

0.542
  pilB Acinetobacter baylyi ADP1

54.321

98.438

0.535
  pilB Legionella pneumophila strain ERS1305867

50.176

98.611

0.495
  pilB Vibrio cholerae strain A1552

52.427

89.41

0.469
  pilF Neisseria gonorrhoeae MS11

46.831

98.611

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

51.829

85.417

0.443
  pilB Vibrio campbellii strain DS40M4

50.607

85.764

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.248

100

0.385
  pilF Thermus thermophilus HB27

36.441

100

0.373