Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IM738_RS01660 Genome accession   NZ_CP063400
Coordinates   352611..354344 (-) Length   577 a.a.
NCBI ID   WP_236964170.1    Uniprot ID   -
Organism   Hydrogenophaga sp. SL48     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347611..359344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM738_RS01630 (IM738_01645) - 348433..348933 (+) 501 WP_236964165.1 NUDIX domain-containing protein -
  IM738_RS01635 (IM738_01650) - 348902..349099 (-) 198 WP_236964166.1 DNA gyrase inhibitor YacG -
  IM738_RS01640 (IM738_01655) zapD 349108..349863 (-) 756 WP_236964167.1 cell division protein ZapD -
  IM738_RS01645 (IM738_01660) coaE 349909..350499 (-) 591 WP_236964168.1 dephospho-CoA kinase -
  IM738_RS01650 (IM738_01665) pilD 350512..351363 (-) 852 WP_236966436.1 A24 family peptidase Machinery gene
  IM738_RS01655 (IM738_01670) pilC 351363..352580 (-) 1218 WP_236964169.1 type II secretion system F family protein Machinery gene
  IM738_RS01660 (IM738_01675) pilB 352611..354344 (-) 1734 WP_236964170.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IM738_RS01670 (IM738_01685) - 354612..355541 (-) 930 WP_236964171.1 polyprenyl synthetase family protein -
  IM738_RS01675 (IM738_01690) rplU 355816..356127 (+) 312 WP_077328645.1 50S ribosomal protein L21 -
  IM738_RS01680 (IM738_01695) rpmA 356141..356398 (+) 258 WP_077328647.1 50S ribosomal protein L27 -
  IM738_RS01685 (IM738_01700) obgE 356528..357601 (+) 1074 WP_236964172.1 GTPase ObgE -
  IM738_RS01690 (IM738_01705) proB 357647..358810 (+) 1164 WP_236964173.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62720.09 Da        Isoelectric Point: 5.8315

>NTDB_id=495427 IM738_RS01660 WP_236964170.1 352611..354344(-) (pilB) [Hydrogenophaga sp. SL48]
MASVDTAPQDTPAMALPGLGRALVSAGKLGQKAAEELYRKAQSGRTSFIAELTGSGAVSPSDLAHTMSTAFAAPLLDLDA
IDVQRLPKGLLDAKICSDYRIVVLSKRNNRLMVATADPADQQAAEKIKFATQLGVDWVIAEYDKLSKLVESQSTTANEAM
DNIVGGDFEFDDIPIDAQDAPEKNAVSEVDDAPVVKFLHKMLLDAFNMRASDLHFEPYEHTYRVRFRVDGELREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPERVIDFRVSTLPTLFGEKIVIRILDPSSAKVGIDALGYEPEEKER
LLSAISRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGINISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQDPD
VIMVGEIRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCPNCKTTLDVPR
KALLDAGFKAEELDGSWTPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILKGGSALDIAQQATREGVRTLRESGLLK
VRQGMTSLEEVLSVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=495427 IM738_RS01660 WP_236964170.1 352611..354344(-) (pilB) [Hydrogenophaga sp. SL48]
ATGGCATCTGTCGATACAGCCCCTCAAGATACACCAGCCATGGCCCTGCCAGGGCTCGGTCGTGCGCTCGTTTCAGCAGG
GAAGCTAGGGCAAAAGGCGGCGGAGGAGCTCTACAGGAAGGCCCAGAGCGGGCGCACCAGCTTCATTGCAGAGTTGACCG
GATCGGGGGCGGTCTCACCTTCAGACCTCGCCCACACGATGTCGACGGCGTTTGCCGCTCCGCTGCTGGATCTTGATGCC
ATTGATGTCCAGCGCCTTCCCAAGGGCTTGCTGGACGCAAAGATTTGTAGCGACTACCGCATTGTTGTGCTGAGCAAGCG
CAACAACCGCTTGATGGTTGCAACAGCGGATCCCGCCGATCAACAGGCGGCTGAAAAAATCAAGTTCGCCACCCAGTTGG
GCGTTGACTGGGTCATTGCCGAATACGACAAGCTGAGCAAGCTGGTGGAGAGCCAGTCGACGACCGCCAATGAAGCGATG
GATAACATCGTTGGCGGCGATTTCGAGTTCGATGACATCCCCATTGATGCACAGGATGCTCCTGAAAAGAATGCGGTGTC
GGAGGTTGATGACGCACCAGTCGTCAAGTTTCTTCACAAGATGCTGCTGGATGCGTTCAACATGCGCGCGTCCGACCTTC
ATTTTGAGCCCTACGAACACACCTACCGTGTGCGCTTTCGCGTGGACGGAGAGCTGAGGGAAATCGCGTCTCCGCCGATC
GCGATCAAGGACAAGCTGGCCTCCCGCATCAAGGTGATTTCTCGTATGGACATCTCCGAAAAGCGGGTGCCACAGGATGG
CCGCATGAAGCTCAAGGTGGGGCCTGAGCGTGTGATCGATTTTCGTGTCAGCACGCTGCCGACGCTGTTCGGCGAGAAGA
TCGTGATCCGTATCCTGGATCCGAGCAGCGCCAAGGTGGGCATTGATGCCCTGGGTTATGAGCCCGAAGAGAAAGAACGG
TTGCTGAGCGCCATTTCGAGACCCTATGGCATGGTTCTGGTGACGGGACCCACCGGTTCCGGAAAAACCGTTTCACTCTA
CACCTGCCTGAACCTGCTGAACAAGCCGGGCATCAACATCTCCACGGCGGAAGACCCTTCGGAAATCAACCTGCCCGGGG
TCAACCAGGTCAACGTCAATGAAAAGGCTGGGCTGACGTTTGCTGCCGCCCTCAAAGCTTTCTTGCGCCAGGATCCAGAT
GTGATCATGGTGGGTGAAATTCGTGACTTGGAAACCGCCGATATTTCCATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCGACACTTCACACCAATGACGCCCCCACCACGTTGACGCGAATGATGAACATGGGCATCCCCACGTTCAACATTGCTT
CCAGTGTGATCCTCATCACCGCACAGCGACTGGCACGCCGACTGTGCCCGAACTGCAAAACGACGCTGGATGTGCCTCGA
AAAGCCTTGCTGGACGCCGGTTTCAAGGCCGAAGAGCTGGATGGCAGCTGGACCCCCTATAAGCCTGTGGGATGTTCTGC
TTGCAACAATGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCTGAGGAAATTCAAAGAATCATTCTTA
AAGGCGGCAGCGCGCTTGACATCGCGCAACAGGCCACGCGGGAAGGTGTCCGGACCTTGCGTGAGTCGGGCCTTCTCAAG
GTCAGGCAAGGCATGACTTCGCTGGAAGAAGTGCTGAGCGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.138

100

0.544
  pilB Acinetobacter baylyi ADP1

52.316

100

0.529
  pilF Neisseria gonorrhoeae MS11

52.566

97.92

0.515
  pilB Legionella pneumophila strain ERS1305867

49.912

98.96

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

45.088

98.787

0.445
  pilB Vibrio campbellii strain DS40M4

44.58

99.133

0.442
  pilB Vibrio cholerae strain A1552

47.776

89.601

0.428
  pilF Thermus thermophilus HB27

38.704

98.96

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.292

93.068

0.366