Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INH39_RS17715 Genome accession   NZ_CP063361
Coordinates   3836808..3838535 (-) Length   575 a.a.
NCBI ID   WP_243488617.1    Uniprot ID   -
Organism   Massilia violaceinigra strain sipir     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3831808..3843535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INH39_RS17695 (INH39_17610) pilD 3832145..3833038 (-) 894 WP_243488613.1 A24 family peptidase Machinery gene
  INH39_RS17700 (INH39_17615) - 3833223..3834299 (+) 1077 WP_243488614.1 acyltransferase -
  INH39_RS17705 (INH39_17620) - 3834601..3835437 (+) 837 WP_243488615.1 DUF3800 domain-containing protein -
  INH39_RS17710 (INH39_17625) pilC 3835570..3836796 (-) 1227 WP_243488616.1 type II secretion system F family protein Machinery gene
  INH39_RS17715 (INH39_17630) pilB 3836808..3838535 (-) 1728 WP_243488617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INH39_RS17720 (INH39_17635) - 3838759..3840039 (-) 1281 WP_243488618.1 HlyC/CorC family transporter -
  INH39_RS17725 (INH39_17640) - 3840216..3840776 (-) 561 WP_243488619.1 ATP-dependent Clp protease proteolytic subunit -
  INH39_RS17730 (INH39_17645) uraH 3840858..3841211 (-) 354 WP_243488620.1 hydroxyisourate hydrolase -
  INH39_RS17735 (INH39_17650) puuE 3841333..3842265 (+) 933 WP_243488621.1 allantoinase PuuE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62471.25 Da        Isoelectric Point: 5.7440

>NTDB_id=494742 INH39_RS17715 WP_243488617.1 3836808..3838535(-) (pilB) [Massilia violaceinigra strain sipir]
MAAVLPNTTPGTPMPGLARALLQAGRLSAQQADSLHKKSLADKSPFIDTLLLTGTFDSRTLAAFCAETFGYPLMDLQSFN
VDALPANAIDGKLMQSQRVIALAKRGNKMSVAISDPTNIQALDQIKFQSEASVEPVIVLHDVLLTLLASLGRNAETSLHD
MVGDQEEIQFGEDDAPAPVVDQGAADIEDAPIVKFLNKILTDAINLGASDIHFEPFEKFYRIRLRVDGMLKEHAQPPITI
RDKLVSRIKVLSKLDISEKRVPQDGRMRLILSPTKAIDFRVSTCPTLFGEKTVMRILDATQAQMGIDALGYDPDQKELLM
EAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNINERSGLTFPVALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVIMITAQRLARRLCTCKKPVETSREIL
LAAGFRDDELEGGWKPYGPVGCERCLGGGYKGRVGIYQIMPITPAIEKIILAHGNSMEIAAQSELEGVKSLRRSGLMKVK
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=494742 INH39_RS17715 WP_243488617.1 3836808..3838535(-) (pilB) [Massilia violaceinigra strain sipir]
ATGGCTGCAGTCCTCCCGAACACGACCCCCGGAACCCCGATGCCGGGCCTGGCCCGCGCCTTGCTGCAGGCGGGCCGCCT
GAGCGCGCAGCAGGCCGATTCGCTGCACAAGAAATCGCTGGCGGACAAGAGTCCTTTCATCGACACCTTGCTGCTGACGG
GCACCTTCGACTCGCGCACCCTGGCCGCTTTTTGCGCGGAAACCTTCGGCTACCCGCTGATGGACTTGCAGAGCTTTAAT
GTCGACGCCCTGCCGGCCAACGCCATCGACGGCAAGCTGATGCAGAGCCAGCGCGTGATCGCCCTGGCCAAGCGCGGCAA
TAAAATGTCGGTGGCCATTTCCGACCCGACCAATATCCAGGCGCTCGACCAGATCAAGTTCCAGAGCGAAGCCTCGGTCG
AGCCGGTGATCGTGCTGCACGACGTGCTGCTGACCTTGCTGGCCAGCCTGGGCCGCAACGCCGAAACCAGCCTCCACGAC
ATGGTGGGCGACCAGGAAGAAATCCAGTTCGGCGAGGACGACGCGCCAGCCCCGGTGGTCGACCAGGGCGCGGCCGACAT
TGAAGACGCGCCGATCGTCAAGTTCCTCAACAAGATCCTGACCGACGCGATCAACTTGGGCGCGTCCGATATCCACTTCG
AACCGTTCGAAAAATTCTACCGCATCCGCCTGCGCGTGGACGGCATGCTCAAGGAGCATGCGCAGCCGCCGATCACCATC
CGCGACAAGCTGGTGTCGCGCATCAAGGTGCTCTCCAAGCTCGACATTTCCGAAAAAAGGGTGCCGCAGGATGGCCGCAT
GCGCCTGATCCTCTCGCCCACCAAGGCCATCGATTTCCGGGTCAGCACCTGCCCCACCCTGTTCGGCGAAAAGACCGTGA
TGCGGATCCTGGACGCGACCCAGGCCCAGATGGGCATCGACGCGCTCGGCTACGATCCGGACCAGAAGGAATTGCTGATG
GAAGCGATCCAGCGTCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCCGGCAAGACGGTGTCGCTGTACACCTG
CCTGAACGTGATCAACAAGCCGGGCATCAATATTTCGACTGCGGAAGACCCGGCCGAGATCAACTTGCCCGGCGTAAACC
AGGTCAACATCAACGAACGCTCCGGACTGACCTTCCCGGTGGCGCTCAAGTCCTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCGGCGCAAACCGGCCACATGGTGTTCTCGAC
CCTGCACACCAACGATGCGCCGTCGACCCTGACGCGCCTGATGAACATGGGCGTGGCGCCCTTTAACATCGCCTCGTCGG
TGATCATGATCACGGCGCAGCGCCTGGCGCGGCGCCTGTGCACCTGCAAGAAACCGGTCGAGACCAGCCGCGAAATCCTG
CTCGCGGCCGGCTTCAGGGACGACGAGCTGGAAGGCGGCTGGAAGCCTTACGGTCCGGTCGGCTGCGAGCGCTGCCTGGG
CGGCGGCTACAAGGGACGGGTCGGTATCTACCAGATCATGCCGATTACCCCGGCGATCGAAAAAATCATCCTGGCGCACG
GCAACTCGATGGAAATCGCCGCGCAATCGGAGCTCGAGGGCGTCAAATCCCTGCGCCGCTCCGGCCTGATGAAAGTAAAG
CAAGGCTTGACCAGCCTTGAAGAAGTTCTCGGCTGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.887

98.435

0.53
  pilB Acinetobacter baylyi ADP1

53.158

99.13

0.527
  pilF Neisseria gonorrhoeae MS11

51.652

100

0.517
  pilB Legionella pneumophila strain ERS1305867

48.221

97.739

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

45.343

98.957

0.449
  pilB Vibrio cholerae strain A1552

45.196

97.739

0.442
  pilB Vibrio campbellii strain DS40M4

44.956

98.261

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.214

97.391

0.372
  pilF Thermus thermophilus HB27

39.439

93.043

0.367