Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFU87_RS03335 Genome accession   NZ_CP074019
Coordinates   703829..705214 (-) Length   461 a.a.
NCBI ID   WP_023156327.1    Uniprot ID   -
Organism   Escherichia coli strain PM22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698829..710214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU87_RS03300 (KFU87_03280) mutT 699275..699664 (+) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -
  KFU87_RS03305 (KFU87_03285) yacG 699764..699961 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  KFU87_RS03310 (KFU87_03290) zapD 699971..700714 (-) 744 WP_001194734.1 cell division protein ZapD -
  KFU87_RS03315 (KFU87_03295) coaE 700714..701334 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  KFU87_RS03320 (KFU87_03300) - 701359..701403 (+) 45 WP_120795372.1 protein YacM -
  KFU87_RS03325 (KFU87_03305) guaC 701559..702602 (+) 1044 WP_001217338.1 GMP reductase -
  KFU87_RS03330 (KFU87_03310) hofC 702637..703839 (-) 1203 WP_000157234.1 protein transport protein HofC -
  KFU87_RS03335 (KFU87_03315) pilB 703829..705214 (-) 1386 WP_023156327.1 type II secretion system protein GspE Machinery gene
  KFU87_RS03340 (KFU87_03320) pilA 705224..705664 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  KFU87_RS03345 (KFU87_03325) nadC 705867..706760 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KFU87_RS03350 (KFU87_03330) ampD 706848..707399 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KFU87_RS03355 (KFU87_03335) ampE 707396..708250 (+) 855 WP_000171999.1 beta-lactamase regulator AmpE -
  KFU87_RS03360 (KFU87_03340) aroP 708293..709666 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50470.20 Da        Isoelectric Point: 6.4532

>NTDB_id=489944 KFU87_RS03335 WP_023156327.1 703829..705214(-) (pilB) [Escherichia coli strain PM22]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLARTLQSALEQRASDIHIEPADNAYRIRMRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=489944 KFU87_RS03335 WP_023156327.1 703829..705214(-) (pilB) [Escherichia coli strain PM22]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAGCTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCATGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio campbellii strain DS40M4

48.062

83.948

0.403
  pilB Vibrio cholerae strain A1552

46.212

85.9

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.373

83.731

0.388
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371