Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KFU87_RS03340 Genome accession   NZ_CP074019
Coordinates   705224..705664 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain PM22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700224..710664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU87_RS03315 (KFU87_03295) coaE 700714..701334 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  KFU87_RS03320 (KFU87_03300) - 701359..701403 (+) 45 WP_120795372.1 protein YacM -
  KFU87_RS03325 (KFU87_03305) guaC 701559..702602 (+) 1044 WP_001217338.1 GMP reductase -
  KFU87_RS03330 (KFU87_03310) hofC 702637..703839 (-) 1203 WP_000157234.1 protein transport protein HofC -
  KFU87_RS03335 (KFU87_03315) pilB 703829..705214 (-) 1386 WP_023156327.1 type II secretion system protein GspE Machinery gene
  KFU87_RS03340 (KFU87_03320) pilA 705224..705664 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  KFU87_RS03345 (KFU87_03325) nadC 705867..706760 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KFU87_RS03350 (KFU87_03330) ampD 706848..707399 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KFU87_RS03355 (KFU87_03335) ampE 707396..708250 (+) 855 WP_000171999.1 beta-lactamase regulator AmpE -
  KFU87_RS03360 (KFU87_03340) aroP 708293..709666 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=489945 KFU87_RS03340 WP_000360895.1 705224..705664(-) (pilA) [Escherichia coli strain PM22]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=489945 KFU87_RS03340 WP_000360895.1 705224..705664(-) (pilA) [Escherichia coli strain PM22]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363