Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFU83_RS18120 Genome accession   NZ_CP073939
Coordinates   3714566..3715951 (+) Length   461 a.a.
NCBI ID   WP_123178101.1    Uniprot ID   -
Organism   Escherichia coli strain MB53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3709566..3720951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU83_RS18095 (KFU83_17980) aroP 3710114..3711487 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  KFU83_RS18100 (KFU83_17985) ampE 3711530..3712384 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KFU83_RS18105 (KFU83_17990) ampD 3712381..3712932 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KFU83_RS18110 (KFU83_17995) nadC 3713020..3713913 (+) 894 WP_001135165.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KFU83_RS18115 (KFU83_18000) pilA 3714116..3714556 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  KFU83_RS18120 (KFU83_18005) pilB 3714566..3715951 (+) 1386 WP_123178101.1 type II secretion system protein GspE Machinery gene
  KFU83_RS18125 (KFU83_18010) hofC 3715941..3717143 (+) 1203 WP_088375710.1 protein transport protein HofC -
  KFU83_RS18130 (KFU83_18015) guaC 3717178..3718221 (-) 1044 WP_001217338.1 GMP reductase -
  KFU83_RS18135 (KFU83_18020) - 3718377..3718421 (-) 45 WP_120795372.1 protein YacM -
  KFU83_RS18140 (KFU83_18025) coaE 3718446..3719066 (+) 621 WP_053889341.1 dephospho-CoA kinase -
  KFU83_RS18145 (KFU83_18030) zapD 3719066..3719809 (+) 744 WP_001194734.1 cell division protein ZapD -
  KFU83_RS18150 (KFU83_18035) yacG 3719819..3720016 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  KFU83_RS18155 (KFU83_18040) mutT 3720348..3720737 (-) 390 WP_000736019.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50606.26 Da        Isoelectric Point: 6.6268

>NTDB_id=488464 KFU83_RS18120 WP_123178101.1 3714566..3715951(+) (pilB) [Escherichia coli strain MB53]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGAARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=488464 KFU83_RS18120 WP_123178101.1 3714566..3715951(+) (pilB) [Escherichia coli strain MB53]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGAGCCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCATTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

84.599

0.39
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369