Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KFU83_RS18115 Genome accession   NZ_CP073939
Coordinates   3714116..3714556 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain MB53     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3709116..3719556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU83_RS18095 (KFU83_17980) aroP 3710114..3711487 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  KFU83_RS18100 (KFU83_17985) ampE 3711530..3712384 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KFU83_RS18105 (KFU83_17990) ampD 3712381..3712932 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KFU83_RS18110 (KFU83_17995) nadC 3713020..3713913 (+) 894 WP_001135165.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KFU83_RS18115 (KFU83_18000) pilA 3714116..3714556 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  KFU83_RS18120 (KFU83_18005) pilB 3714566..3715951 (+) 1386 WP_123178101.1 type II secretion system protein GspE Machinery gene
  KFU83_RS18125 (KFU83_18010) hofC 3715941..3717143 (+) 1203 WP_088375710.1 protein transport protein HofC -
  KFU83_RS18130 (KFU83_18015) guaC 3717178..3718221 (-) 1044 WP_001217338.1 GMP reductase -
  KFU83_RS18135 (KFU83_18020) - 3718377..3718421 (-) 45 WP_120795372.1 protein YacM -
  KFU83_RS18140 (KFU83_18025) coaE 3718446..3719066 (+) 621 WP_053889341.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=488463 KFU83_RS18115 WP_000360901.1 3714116..3714556(+) (pilA) [Escherichia coli strain MB53]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=488463 KFU83_RS18115 WP_000360901.1 3714116..3714556(+) (pilA) [Escherichia coli strain MB53]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363