Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SBAL175_RS02495 Genome accession   NC_017571
Coordinates   550291..552003 (-) Length   570 a.a.
NCBI ID   WP_006083350.1    Uniprot ID   -
Organism   Shewanella baltica BA175     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 545291..557003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBAL175_RS02465 (Sbal175_0481) mutT 545762..546154 (-) 393 WP_006083356.1 8-oxo-dGTP diphosphatase MutT -
  SBAL175_RS02470 (Sbal175_0482) yacG 546231..546440 (-) 210 WP_006083355.1 DNA gyrase inhibitor YacG -
  SBAL175_RS02475 (Sbal175_0483) zapD 546514..547248 (-) 735 WP_006083354.1 cell division protein ZapD -
  SBAL175_RS02480 (Sbal175_0484) coaE 547241..547858 (-) 618 WP_006083353.1 dephospho-CoA kinase -
  SBAL175_RS02485 (Sbal175_0485) pilD 547898..548824 (-) 927 WP_006083352.1 A24 family peptidase Machinery gene
  SBAL175_RS02490 (Sbal175_0486) pilC 548866..550137 (-) 1272 WP_006083351.1 type II secretion system F family protein Machinery gene
  SBAL175_RS02495 (Sbal175_0487) pilB 550291..552003 (-) 1713 WP_006083350.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SBAL175_RS02500 (Sbal175_0488) - 552090..552467 (-) 378 WP_006083349.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SBAL175_RS02505 (Sbal175_0489) nadC 553177..554058 (-) 882 WP_006083348.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SBAL175_RS02510 (Sbal175_0490) ampD 554642..555205 (+) 564 WP_006083347.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SBAL175_RS02515 (Sbal175_0491) ampE 555275..556126 (+) 852 WP_006083346.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62861.31 Da        Isoelectric Point: 6.2570

>NTDB_id=48819 SBAL175_RS02495 WP_006083350.1 550291..552003(-) (pilB) [Shewanella baltica BA175]
MPTSGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIAELCYEEYGTPLLDLNEFDIASIPEE
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDRRNEESTGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQAQITQSFTTFKPVGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVIHGVTSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=48819 SBAL175_RS02495 WP_006083350.1 550291..552003(-) (pilB) [Shewanella baltica BA175]
ATGCCCACTTCAGGTCTTCATCTAGGTTTATCGACCCTGTTTATCCGTAAAGGTCTGCTAAATGAAGAGCAGATGGCTAA
CGCCATCACTAAATCTCGGCAAAATAAACATGCCCTTGTTAGTACGTTAGTTCAAAGTAAGCTGGTGTCAGCTCGCGCAA
TTGCCGAATTGTGCTACGAAGAATACGGCACCCCACTACTCGATCTTAATGAATTTGATATTGCTTCCATTCCTGAAGAA
TTTCTGAATAAGAAACTCATTGAAAAACACCGTTGCTTACCCTTGTTTAAACGCGGTAATCGCCTCTATATAGGTACATC
TGACCCGACTAATATTGCAGCCCTCGAAGATTTCCAATTTAGTGCAGGCCTGCATGCCGAAGCGATTCTGGTTGAAGAAG
ATAAACTTGCTAAGGCGCTGGAAAAAATTCTCGAAGAAGATATCAGTGGTCTCGATTTAGGCGGCATGGATGAAGCGGCC
CTGGCCGCGATAGAAATTACTGACACCGACAGACGCAATGAGGAGTCTACAGGTGAGAGTAGCGACGATGCGCCTATTGT
CATTTATATCAATAAAATTTTAACCGATGCCATTCGCAAGGGTGCATCGGATTTACATTTTGAGCCCTATGAGAAACGCT
ACCGTATACGTTTCCGTATAGATGGTATTCTGCACGAAGTCTCTGAACCGCCGATTAGCCTCGCTGGTCGTTTATCGGCA
CGCCTAAAGGTAATGTCGAAACTCGATATCGCCGAACGGCGCGTGCCGCAGGATGGTCGAATTAAGATGAAACTATCCCG
TACTAAGTCCATCGACTTTCGGGTAAGCACCTTGCCGACGCTCTGGGGCGAAAAAATTGTAATGCGGATTTTGGACTCAT
CCTCCGCACAATTAGGTATTGAAAAGCTTGGCTACGAGCCCGACCAAGAAAAGCTCTACCTTGAGATGCTGGCAAAACCA
CAAGGGATGATTCTAGTGACTGGTCCAACCGGTTCAGGTAAAACAGTCTCCCTCTATACAGGGTTAAATATTCTCAACAC
CGAAGAGCGTAATATTTCTACCGCTGAAGATCCGGTTGAGATTAACCTTGAAGGCGTGAATCAGGTTCATATTAATTTAA
AAGCAGGGTTAACCTTCGCGTCCGCTTTACGTTCATTTCTACGCCAAGACCCCGATGTGGTGATGGTCGGGGAAATCCGC
GATCTTGAAACCGCCGAAATTGCCATTAAAGCAGCGCAAACGGGTCACTTAGTCTTATCAACCTTGCACACAAACTCGGC
TGCCGAAACCTTAACCCGTTTAATCAACATGGGTGTACCTGGCTATAACATAGCTAGTTCAGTGAATCTGATTATTGCCC
AACGCTTAGCGCGTCGCCTCTGCCCAGAATGTAAACAGGCTGAAGTGATACCAGAACATGAATTACTACGCCTAGGTTTT
GCCCAAGCACAAATTACTCAAAGCTTTACCACCTTTAAACCTGTTGGCTGTGAGCATTGCTCCGCGGGCTACAAGGGACG
GGTTGGCATTTATGAAGTAATGAAAATGTCCGATGAAATTGCCCGCACTATCATGGAGGGTGGTAACTCATTGCAAATAG
CCTCCCAAGCTAAAGCGCAAGGAATGCGAGATTTACGACAATCCGGGTTATTGAAAGTGATCCACGGTGTCACCAGCATT
GCAGAAGTTAATCGTGTAACCAGTTTTGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.18

98.421

0.582
  pilB Acinetobacter baumannii D1279779

58.899

95.614

0.563
  pilB Legionella pneumophila strain ERS1305867

56.228

98.596

0.554
  pilB Vibrio cholerae strain A1552

53.333

97.368

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.761

99.649

0.516
  pilB Vibrio campbellii strain DS40M4

51.504

99.123

0.511
  pilF Neisseria gonorrhoeae MS11

47.527

99.298

0.472
  pilF Thermus thermophilus HB27

38.681

98.421

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.351

97.895

0.375

Multiple sequence alignment