Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IHQ43_RS24810 Genome accession   NZ_CP062253
Coordinates   5577261..5578961 (+) Length   566 a.a.
NCBI ID   WP_192562435.1    Uniprot ID   -
Organism   Pseudomonas gozinkensis strain IzPS32d     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5572261..5583961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHQ43_RS24790 - 5572548..5575499 (+) 2952 WP_192562431.1 DUF748 domain-containing protein -
  IHQ43_RS24795 - 5575499..5575795 (+) 297 WP_192562432.1 DUF2845 domain-containing protein -
  IHQ43_RS24800 - 5575964..5576320 (-) 357 WP_192562433.1 BON domain-containing protein -
  IHQ43_RS24805 - 5576618..5577034 (-) 417 WP_192562434.1 pilin -
  IHQ43_RS24810 pilB 5577261..5578961 (+) 1701 WP_192562435.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHQ43_RS24815 pilC 5578964..5580181 (+) 1218 WP_192562436.1 type II secretion system F family protein Machinery gene
  IHQ43_RS24820 pilD 5580183..5581055 (+) 873 WP_192562437.1 A24 family peptidase Machinery gene
  IHQ43_RS24825 coaE 5581052..5581675 (+) 624 WP_192562438.1 dephospho-CoA kinase -
  IHQ43_RS24830 yacG 5581672..5581872 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  IHQ43_RS24835 - 5581879..5582094 (-) 216 WP_007959584.1 hypothetical protein -
  IHQ43_RS24840 - 5582160..5582849 (-) 690 WP_192562439.1 energy-coupling factor ABC transporter permease -
  IHQ43_RS24845 - 5582983..5583609 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62691.72 Da        Isoelectric Point: 5.9475

>NTDB_id=487395 IHQ43_RS24810 WP_192562435.1 5577261..5578961(+) (pilB) [Pseudomonas gozinkensis strain IzPS32d]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESVEDGKQDTLGGVDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVMRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDI
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPAGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=487395 IHQ43_RS24810 WP_192562435.1 5577261..5578961(+) (pilB) [Pseudomonas gozinkensis strain IzPS32d]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAACTGGTCCAGGCCGAACTGCTCACGGAAAAGAGTGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTACAGAACAAACTGGTAAAGAGCGCGCAGGTCG
CCGAGATTGCCTCGGAGCACTTCGGCATGGCGTTCCTCGACCTCAACTGCCTCGACAAGGAAACCCAGCCAAAGGGCCTG
GTCAGTGAAAAACTGGTACGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGATCTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACGGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAAAAATTCTTCGACACCCACGCCACCGGCCTGGAGGAAATGGCGGATGTCGATCTCGACGGG
CTGGATATCGAATCGGTTGAAGACGGCAAGCAGGACACCCTCGGCGGGGTCGATGCGGACGATGCCCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCTTATGAAAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAGGTGGCCAAACCGCCGATTCAGCTCGCCGGGCGCATCGCCGCGCGCCTG
AAGGTCATGGCGAGCCTCGACATTTCCGAACGACGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGACTTTCGGGTCAACACCCTGCCCACGCTCTGGGGCGAGAAAGTGGTGATGCGGATCCTCGACCCCTCCAGCG
CCCAGATCGGCATCGATGCCCTCGGTTACGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAACAGCCGCAAGGC
ATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCACGCCAGG
GGATGGACTTTGCCCAGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACATC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGGCACCTCGTGCTGTCCACCCTGCACACCAACAGCGCGGCGGA
AACCCTAACCCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCGACCTCGGTGAGCCTGATCATTGCCCAGCGCC
TGGCGCGAAAACTCTGCAGCCATTGCAAGAAACCCATCGAAATCCCTCGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCGCCGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGAAT
TTATGAAGTGGTGAAGAACACCCCCGAACTGCAACGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGATACCCAGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACTTCGGGCCTGATAAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

99.823

0.58
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.383

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.438

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.668

99.47

0.484
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.623

93.64

0.371
  pilF Thermus thermophilus HB27

36.918

98.587

0.364