Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IF199_RS25675 Genome accession   NZ_CP062252
Coordinates   5599255..5600955 (+) Length   566 a.a.
NCBI ID   WP_096820893.1    Uniprot ID   -
Organism   Pseudomonas allokribbensis strain IzPS23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5594255..5605955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF199_RS25655 - 5594517..5597468 (+) 2952 WP_192559018.1 DUF748 domain-containing protein -
  IF199_RS25660 - 5597468..5597764 (+) 297 WP_102621087.1 DUF2845 domain-containing protein -
  IF199_RS25665 - 5597933..5598289 (-) 357 WP_096820895.1 BON domain-containing protein -
  IF199_RS25670 - 5598588..5599028 (-) 441 WP_096820894.1 pilin -
  IF199_RS25675 pilB 5599255..5600955 (+) 1701 WP_096820893.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IF199_RS25680 pilC 5600958..5602175 (+) 1218 WP_192559019.1 type II secretion system F family protein Machinery gene
  IF199_RS25685 pilD 5602177..5603049 (+) 873 WP_192559020.1 A24 family peptidase Machinery gene
  IF199_RS25690 coaE 5603046..5603669 (+) 624 WP_096820890.1 dephospho-CoA kinase -
  IF199_RS25695 yacG 5603666..5603866 (+) 201 WP_192559021.1 DNA gyrase inhibitor YacG -
  IF199_RS25700 - 5603875..5604090 (-) 216 WP_096820888.1 hypothetical protein -
  IF199_RS25705 - 5604156..5604845 (-) 690 WP_192559022.1 energy-coupling factor ABC transporter permease -
  IF199_RS25710 - 5604979..5605605 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -
  IF199_RS25715 - 5605750..5605923 (+) 174 WP_096820886.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62497.43 Da        Isoelectric Point: 5.5538

>NTDB_id=487366 IF199_RS25675 WP_096820893.1 5599255..5600955(+) (pilB) [Pseudomonas allokribbensis strain IzPS23]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLTTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPAGCDQCNGGYKGRTGIYEVVKNTPELQRLIMAEGNSLEIDSQMRLDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=487366 IF199_RS25675 WP_096820893.1 5599255..5600955(+) (pilB) [Pseudomonas allokribbensis strain IzPS23]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTGGCAGCAGGCCCAGCGCAATCGTCTGTCGCTGGTCAGCTATCTGGTACAGAACAAACTGGTGAAGAGTTCGCAGGTTG
CCGAGATCGCCTCGGAGCATTTCGGCATGGCGTTCCTCGACCTCAACTGCCTGGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCGCTGCCCCTGTGGCGGCGCGGCAACAAGCTGTTTGTGGGGATCTCCGA
CCCGAGCAATCATCAGGCAATCAACGATATTCAGTTCAGCACCGGGCTGACCACCGAAGCGATTCTGGTGGAAGACGACA
AGCTCACCGACGCCATCGAGAAGTTCTTCGACACCCACGCCTCCGGCCTGGAAGACATGGCCGATGTCGACCTCGACGGG
CTGGACGTCGAGTCCATCGATGACAGCAAGCAGGACGCCATCGGTGGGCTGGACGCCGACGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGCTGCTGGACGCGATCAAGAGCGGTTCTTCCGACCTGCACTTCGAACCCTACGAAAAAAACTACCGGG
TGCGCGTACGTACCGACGGCATCCTGCGCGAAGTGGCCAAGCCGCCGATTCAACTGGCCGGGCGCATTGCCGCACGCCTG
AAGGTCATGGCCAGCCTCGACATTTCGGAACGCCGCAAACCTCAGGACGGGCGACTGAAGATGCGCCTGTCCAAGAGCAA
GTCGATCGATTTCCGGGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAGATAGGCATCGACGCCCTCGGCTACGAGCCGGAGCAGAAAGACCTGTACCTCGCTGCCCTGAAGCAACCACAAGGT
ATGATCCTGGTCACCGGTCCCACCGGTTCCGGCAAGACCGTGTCGCTGTACACCGGGCTCAACATTCTCAATACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCTAAGCAGG
GGCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGATCTC
GAGACTGCGGAAATCGCCATCAAGGCAGCGCAGACCGGTCACCTTGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
GACACTCACCCGCTTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACGTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCACTGCAAAAAGCCCATCGAGATTCCCCGCGAAACCTTGCTCAAGGAAGGTTTTCCCGAG
GAACGCATCGGCAGTTTCACGATCTATGAGCCCGCCGGTTGCGATCAATGCAACGGCGGCTACAAGGGACGCACCGGGAT
TTATGAAGTGGTGAAAAACACCCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCATTGGAAATCGACAGCCAGA
TGCGCCTGGACGGCTTCAACGACCTGCGCACCTCGGGACTGATCAAGGCGATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.823

0.581
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.184

100

0.562
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio campbellii strain DS40M4

49.474

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

50.267

99.117

0.498
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362