Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IF199_RS02175 Genome accession   NZ_CP062252
Coordinates   480046..482124 (+) Length   692 a.a.
NCBI ID   WP_192559573.1    Uniprot ID   -
Organism   Pseudomonas allokribbensis strain IzPS23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475046..487124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF199_RS02155 pilM 477261..478325 (+) 1065 WP_102620205.1 pilus assembly protein PilM Machinery gene
  IF199_RS02160 - 478325..478891 (+) 567 WP_192559571.1 PilN domain-containing protein -
  IF199_RS02165 pilO 478888..479511 (+) 624 WP_192559572.1 type 4a pilus biogenesis protein PilO -
  IF199_RS02170 - 479508..480032 (+) 525 WP_096819917.1 pilus assembly protein PilP -
  IF199_RS02175 pilQ 480046..482124 (+) 2079 WP_192559573.1 type IV pilus secretin PilQ Machinery gene
  IF199_RS02180 aroK 482129..482647 (+) 519 WP_045121685.1 shikimate kinase AroK -
  IF199_RS02185 aroB 482790..483890 (+) 1101 WP_096819915.1 3-dehydroquinate synthase -
  IF199_RS02190 - 483901..485499 (+) 1599 WP_192559574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75096.26 Da        Isoelectric Point: 5.4082

>NTDB_id=487353 IF199_RS02175 WP_192559573.1 480046..482124(+) (pilQ) [Pseudomonas allokribbensis strain IzPS23]
MNRIFSTLGFSLWIALLSPMVQAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARTATVVEANDRTRLIINLTQLAPYDARVEGNTLFVVVGQGSKVTAPRPATKAPRATPVAAPARTFAPTAKAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFSRTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=487353 IF199_RS02175 WP_192559573.1 480046..482124(+) (pilQ) [Pseudomonas allokribbensis strain IzPS23]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTTCAAGCGGCCAATCTGAA
GACGCTGGACGTTGCGGCGCTGCCGGGCGATCGCGTCGAGTTGAAGCTGTCGTTCGACGGCCCACCGCCACAGCCCAAGG
GCTACACCACCGATTCGCCTGCGCGGATTGCTCTGGATCTGCCGGGAGTGGCCAGCCAGCTCGCGAGCAAGACTCGCGAC
CTCGGTAGCGGAAATGCGCGCACTGCCACAGTGGTCGAGGCCAACGACCGTACCCGGTTGATCATCAACCTGACGCAGTT
GGCGCCGTATGACGCCCGGGTCGAGGGGAACACGCTGTTCGTGGTGGTCGGGCAGGGCAGCAAAGTTACTGCGCCGCGAC
CTGCTACCAAGGCTCCGCGCGCCACGCCGGTGGCCGCCCCGGCCCGGACGTTTGCGCCTACCGCCAAGGCCATCCGTGGC
GTGGACTTCCAGCGTGGCACGGCGGGTGAGGGCAATGTCGTCATCGACCTGTCCGACCCGACCATTGCGCCGGACATCCA
GGAACATGACGGCAAGATCATCCTCAATTTTTCCCGCACCCAATTGCCCGAGCCGTTGCGGGTGCGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCTGGCGTCAGCGGGGATCGCGCGACCATCACCGTCGAGCCCAGCGGCACC
TTCGACTATTCCACTTACCAGACCGACAACAAGCTGACCGTGAGCATCCGGCCGTTGACCGTCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTACAGCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGGATCACCTTGCGCCTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTGGC
GCCGGCGGATGAAATCGCTGCCCGCGAACGGCAGGAACTGGAATCGCAGAAACAGATCGCCGAGCTGGCACCGCTGCGCC
GCGAGCTGCTGCAAGTGAATTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCCAAA
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGCACCAACAACATCATCGCCTACCAGACCCAGGATCGCCTCGACGA
GCTGCGGCGGATCGTGGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAAGCCAACGTCG
ATTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGATCGATCCAGAACAAGGGCAACTGGAATACTTCCGGGGTCAGCAAC
GGCTCGTCGACCACCATCGGTACACCGGGGAGCACCAGCACCAACTCACCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGGATCGGCATTGCGTTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACGGCGATGGAGAAGACCGGCAACG
GTGAAATCGTCTCGCAGCCCAAGGTCGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAAATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCTTTCAAGGAGGCCTCGCTGTCGCTGGAGGTCACGCCGCAGATCAC
TCCGGACAACCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGCGGGGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.888

100

0.743
  comQ Acinetobacter baylyi ADP1

41.27

100

0.413
  pilQ Acinetobacter baumannii strain A118

37.784

100

0.409
  pilQ Acinetobacter baumannii D1279779

37.784

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.222

97.543

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.187

97.254

0.371