Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IF199_RS02155 Genome accession   NZ_CP062252
Coordinates   477261..478325 (+) Length   354 a.a.
NCBI ID   WP_102620205.1    Uniprot ID   -
Organism   Pseudomonas allokribbensis strain IzPS23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472261..483325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF199_RS02145 - 473159..474427 (+) 1269 WP_096819922.1 malic enzyme-like NAD(P)-binding protein -
  IF199_RS02150 - 474597..477044 (-) 2448 WP_102620206.1 penicillin-binding protein 1A -
  IF199_RS02155 pilM 477261..478325 (+) 1065 WP_102620205.1 pilus assembly protein PilM Machinery gene
  IF199_RS02160 - 478325..478891 (+) 567 WP_192559571.1 PilN domain-containing protein -
  IF199_RS02165 pilO 478888..479511 (+) 624 WP_192559572.1 type 4a pilus biogenesis protein PilO -
  IF199_RS02170 - 479508..480032 (+) 525 WP_096819917.1 pilus assembly protein PilP -
  IF199_RS02175 pilQ 480046..482124 (+) 2079 WP_192559573.1 type IV pilus secretin PilQ Machinery gene
  IF199_RS02180 aroK 482129..482647 (+) 519 WP_045121685.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38164.71 Da        Isoelectric Point: 4.7582

>NTDB_id=487352 IF199_RS02155 WP_102620205.1 477261..478325(+) (pilM) [Pseudomonas allokribbensis strain IzPS23]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLRIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAMQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=487352 IF199_RS02155 WP_102620205.1 477261..478325(+) (pilM) [Pseudomonas allokribbensis strain IzPS23]
GTGCTAGGACTCTTCAACAAAAAGACCAACACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAGAATA
TCGCCGAACTCGAAGGCGTCGGGCACGCCTTGTCCCGGGTGCTGGTCAAGGCGCGTACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGCTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAGGATCGAGGCCGATCAATACATCCCCTATCCGCTGGACGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCACCGC
GCAACCCCGAACGGGTCAATGTGTTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAGGCGGCACTGGCCCTC
GCCGGCCTCACTGCGCGGGTGGTGGATGTCGAGGCGTATGCGCTCGAGCGCTCGTTCGGCTTGCTGGCCATGCAGCTGGC
GGCTTCCCAGGAGCGCCTGACCGTTGCGGTGGTCGATATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAGCAACTGTTTGGCGGGCGCCAACTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCATT
GAACAGGCCGGGCTGGCCAAAAAGCAGGGCGGTCTGCCGGACGATTATGTCAGCGAAGTCCTGCAGCCCTTTCGCGAGGC
GCTGGTGCAGCAGGTCTCGCGTTCCCTGCAATTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCACATTCTGCTGG
CCGGCGGTACGGCGTCGGTGCCCGGGCTGGATCGGCTGATCGAAGAGCGCCTGGGCACGCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489