Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IF189_RS02105 Genome accession   NZ_CP062251
Coordinates   469160..471238 (+) Length   692 a.a.
NCBI ID   WP_134825218.1    Uniprot ID   A0A4Y8VT05
Organism   Pseudomonas sp. IzPS59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 464160..476238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF189_RS02085 pilM 466375..467439 (+) 1065 WP_134825214.1 pilus assembly protein PilM Machinery gene
  IF189_RS02090 - 467439..468005 (+) 567 WP_192552747.1 PilN domain-containing protein -
  IF189_RS02095 pilO 468002..468625 (+) 624 WP_085711057.1 type 4a pilus biogenesis protein PilO -
  IF189_RS02100 - 468622..469146 (+) 525 WP_134825217.1 pilus assembly protein PilP -
  IF189_RS02105 pilQ 469160..471238 (+) 2079 WP_134825218.1 type IV pilus secretin PilQ Machinery gene
  IF189_RS02110 aroK 471243..471761 (+) 519 WP_011332081.1 shikimate kinase AroK -
  IF189_RS02115 aroB 471904..473004 (+) 1101 WP_119429530.1 3-dehydroquinate synthase -
  IF189_RS02120 - 473015..474613 (+) 1599 WP_114881134.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75143.32 Da        Isoelectric Point: 5.4082

>NTDB_id=487325 IF189_RS02105 WP_134825218.1 469160..471238(+) (pilQ) [Pseudomonas sp. IzPS59]
MNRIFSTLGFSLWIALLSPMVYAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLATKTRD
LGSGNARSATVVEANDRTRLIINLTQLAPYDARVEGNTLFVVVGQGTKASAPRPAAKTPRATPVAAPTRTYAPVARAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPGGT
FDYSTYQTDNKLTVSIRPLTIDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=487325 IF189_RS02105 WP_134825218.1 469160..471238(+) (pilQ) [Pseudomonas sp. IzPS59]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTTACGCAGCCAACCTGAA
GACGCTGGATGTGGCGGCGCTGCCGGGTGATCGCGTCGAGCTGAAACTGTCGTTCGACGGCCCGCCGCCGCAGCCCAAGG
GTTACACCACCGATTCGCCGGCGCGGATCGCCCTCGATCTGCCGGGAGTGGCCAGCCAGCTCGCGACCAAGACCCGCGAT
CTCGGTAGCGGCAATGCCCGCTCAGCCACGGTGGTCGAGGCCAATGACCGTACCCGGTTGATCATCAACCTGACGCAACT
GGCGCCGTATGACGCCCGGGTTGAGGGCAACACGCTGTTCGTGGTGGTCGGGCAGGGCACCAAGGCCAGCGCGCCGCGAC
CTGCCGCGAAGACACCGCGTGCCACGCCGGTGGCAGCGCCGACGCGGACTTACGCACCCGTCGCCAGGGCCATTCGTGGC
GTGGACTTCCAGCGCGGCACGGCGGGTGAGGGCAATGTCGTGATCGACCTGTCCGACCCGACCATCGCACCGGACATCCA
GGAACACGACGGCAAGATCATCCTCAATTTCGCCCGCACCCAGTTGCCGGAACCGCTGCGGGTGCGTCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGCGTCAGCGGGGATCGGGCGACCATCACCGTCGAGCCCGGTGGCACG
TTCGACTATTCCACCTACCAGACCGACAACAAGCTGACGGTGAGTATCCGTCCGCTGACCATCGACGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTACAGCGGTGAAAAGCTTTCGTTGAATTTCCAGGACATCGATGTGCGCTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAACCTGGTGGCCAGCGACACGGTGCAGGGCGGGATCACCTTGCGCCTGCAAAACGTG
CCGTGGGATCAGGCACTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTTTTACTGGTGGC
GCCGGCGGATGAAATCGCCGCCCGCGAACGGCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCGCTGCGTC
GTGAGCTGTTGCAGGTGAACTACGCCAAGGCGGCAGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCCAAG
GTCGACGAACGGGGTTCGATCACTGTCGACGAGCGCACCAACAACATCATCGCCTACCAGACCCAGGATCGCCTCGACGA
ACTGCGGCGGATTGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGCCTGGGTGTGCGCTGGGGCGGGTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAT
GGCTCGTCGACCACCATCGGCACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
GTCCGGGATCGGCATCGCGTTCATCACCGACAACGTCTTGCTGGACCTTGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAAATCGTCTCGCAACCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTCACGCCGCAGATTAC
TCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGCGGGGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y8VT05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.744
  comQ Acinetobacter baylyi ADP1

41.27

100

0.413
  pilQ Acinetobacter baumannii D1279779

37.534

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.534

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.83

98.555

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.89

97.254

0.368