Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IF189_RS02085 Genome accession   NZ_CP062251
Coordinates   466375..467439 (+) Length   354 a.a.
NCBI ID   WP_134825214.1    Uniprot ID   A0A4Y8VTE9
Organism   Pseudomonas sp. IzPS59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 461375..472439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF189_RS02075 - 462280..463548 (+) 1269 WP_134825212.1 malic enzyme-like NAD(P)-binding protein -
  IF189_RS02080 - 463711..466158 (-) 2448 WP_192552746.1 penicillin-binding protein 1A -
  IF189_RS02085 pilM 466375..467439 (+) 1065 WP_134825214.1 pilus assembly protein PilM Machinery gene
  IF189_RS02090 - 467439..468005 (+) 567 WP_192552747.1 PilN domain-containing protein -
  IF189_RS02095 pilO 468002..468625 (+) 624 WP_085711057.1 type 4a pilus biogenesis protein PilO -
  IF189_RS02100 - 468622..469146 (+) 525 WP_134825217.1 pilus assembly protein PilP -
  IF189_RS02105 pilQ 469160..471238 (+) 2079 WP_134825218.1 type IV pilus secretin PilQ Machinery gene
  IF189_RS02110 aroK 471243..471761 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38076.54 Da        Isoelectric Point: 4.7582

>NTDB_id=487324 IF189_RS02085 WP_134825214.1 466375..467439(+) (pilM) [Pseudomonas sp. IzPS59]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGHALSRVLAKARTGLRSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVGEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=487324 IF189_RS02085 WP_134825214.1 466375..467439(+) (pilM) [Pseudomonas sp. IzPS59]
GTGCTAGGACTCTTCAATAAAAAGACCAATACGTTGCTGGGGATCGATATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGAACTCGAGGGCGTCGGGCATGCCCTGTCCCGGGTGCTGGCCAAGGCGCGCACCGGGCTCAGGAGCGTGGCGGTG
GCGGTGGCCGGCTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTACCCGCTGGACGAAGTCGCCATCGACTTCGAAGTCCAAGGCGTTTCGCCCC
GCAATCCCGAGCGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAGGCGGCGCTGGCCCTT
GCCGGCCTGACGGCGCGGGTGGTGGATGTCGAGGCGTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCCACGCAACTGGC
GGCCTCCCAGGAGCGCCTGACGGTCGCGGTGGTCGATATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGGC
GGATCATCTACACCCGCGAGCAACTGTTCGGCGGGCGCCAGCTGACCGAGGAAATCCAGCGTCGTTATGGCCTGACCCTC
GAGCAGGCCGGGCTGGCCAAGAAGCAGGGCGGTCTGCCGGACGATTATGTCGGCGAGGTCCTGCAGCCGTTTCGCGAGGC
GCTGGTACAACAGGTGTCGCGTTCCCTGCAGTTCTTCTTTGCTTCCGGCCAGTACAACGCGGTCGATCATATTCTGCTGG
CCGGCGGCACGGCCTCGGTGCCCGGGCTGGACCGGCTGATCGAAGAGCGCCTCGGCACCCCGACCCAGGTCGCCAATCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCTTTGGCCAGTGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y8VTE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486