Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   KCG55_RS10565 Genome accession   NZ_CP073117
Coordinates   420544..421017 (+) Length   157 a.a.
NCBI ID   WP_283255525.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415544..426017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG55_RS02005 (KCG55_02015) - 416329..416625 (+) 297 WP_003684318.1 helix-turn-helix transcriptional regulator -
  KCG55_RS02010 (KCG55_02020) glnD 416645..419203 (+) 2559 WP_254323241.1 [protein-PII] uridylyltransferase -
  KCG55_RS02015 (KCG55_02025) pilA2 419997..420449 (+) 453 WP_254323242.1 pilin Machinery gene
  KCG55_RS10565 (KCG55_02030) pilA/pilA1 420544..421017 (+) 474 WP_283255525.1 pilin Machinery gene
  KCG55_RS02030 (KCG55_02035) - 421439..421921 (+) 483 WP_003684427.1 Lrp/AsnC family transcriptional regulator -
  KCG55_RS02035 (KCG55_02040) - 422039..422635 (+) 597 WP_254323243.1 DUF1415 domain-containing protein -
  KCG55_RS02040 (KCG55_02045) - 422650..423009 (+) 360 WP_063076686.1 YbaN family protein -
  KCG55_RS02045 (KCG55_02050) - 423455..424873 (+) 1419 WP_254323244.1 sodium:proton antiporter -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16917.44 Da        Isoelectric Point: 5.0787

>NTDB_id=487026 KCG55_RS10565 WP_283255525.1 420544..421017(+) (pilA/pilA1) [Neisseria subflava strain HP0015]
MNTPLQAQKGFTLIELMIVIAILGILVVIAVPTYQNYIGRAQASEALYLADDLKTEISIASAINNRLPNAEDVSKQGAIG
VTAQRIGGTYIQNGGVTVEADTGKISIPFDKGQNKGKVLTLTPYRINNDSTWLFNWQCGGTLDPMMIPAMCRDNSNG

Nucleotide


Download         Length: 474 bp        

>NTDB_id=487026 KCG55_RS10565 WP_283255525.1 420544..421017(+) (pilA/pilA1) [Neisseria subflava strain HP0015]
ATGAATACCCCACTTCAAGCCCAAAAAGGTTTTACCCTGATTGAGCTGATGATAGTTATCGCCATCCTCGGTATTCTGGT
TGTTATCGCCGTGCCTACCTACCAAAACTATATCGGCAGGGCGCAAGCCTCCGAAGCACTATACCTTGCCGATGACCTGA
AAACCGAAATCAGCATTGCCTCAGCGATAAATAACCGCCTGCCCAATGCAGAGGATGTCAGCAAACAAGGCGCAATCGGC
GTTACCGCTCAACGCATCGGTGGCACATACATCCAAAACGGTGGCGTAACTGTAGAAGCAGATACAGGAAAAATCAGTAT
TCCTTTCGATAAAGGGCAAAACAAAGGCAAAGTCCTGACCCTGACCCCTTATCGAATTAACAATGACTCGACTTGGTTGT
TTAACTGGCAATGCGGCGGAACACTGGATCCGATGATGATTCCCGCCATGTGCCGAGATAACTCAAACGGATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(40-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

42.667

95.541

0.408
  pilE Neisseria gonorrhoeae MS11

34.682

100

0.382
  comP Acinetobacter baylyi ADP1

38.411

96.178

0.369
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.926

94.904

0.369
  pilA Pseudomonas aeruginosa PAK

36.306

100

0.363
  pilA2 Legionella pneumophila str. Paris

38.255

94.904

0.363
  pilA2 Legionella pneumophila strain ERS1305867

38.255

94.904

0.363