Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IHE26_RS02035 Genome accession   NZ_CP062196
Coordinates   424347..425867 (-) Length   506 a.a.
NCBI ID   WP_192437103.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain P5462     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419347..430867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE26_RS02005 (IHE26_02005) - 420068..420604 (-) 537 WP_192437098.1 septation protein IspZ -
  IHE26_RS02010 (IHE26_02010) - 420652..420960 (-) 309 WP_192437099.1 PqqD family protein -
  IHE26_RS02015 (IHE26_02015) - 420983..421204 (-) 222 WP_192437100.1 hypothetical protein -
  IHE26_RS02020 (IHE26_02020) - 421201..421407 (-) 207 WP_192437101.1 hypothetical protein -
  IHE26_RS02025 (IHE26_02025) - 421417..422457 (-) 1041 WP_192437102.1 radical SAM protein -
  IHE26_RS02030 (IHE26_02030) - 423109..424344 (-) 1236 WP_106911398.1 type II secretion system F family protein -
  IHE26_RS02035 (IHE26_02035) pilB 424347..425867 (-) 1521 WP_192437103.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHE26_RS02040 (IHE26_02040) - 425928..426386 (-) 459 WP_192437104.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IHE26_RS02045 (IHE26_02045) nadC 427157..428083 (-) 927 WP_192437105.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IHE26_RS02050 (IHE26_02050) ampD 428520..429125 (+) 606 WP_192437106.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHE26_RS02055 (IHE26_02055) ampE 429201..430043 (+) 843 WP_192437107.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55908.20 Da        Isoelectric Point: 6.2025

>NTDB_id=486822 IHE26_RS02035 WP_192437103.1 424347..425867(-) (pilB) [Plesiomonas shigelloides strain P5462]
MKIENPTELKKYVSFSSLITKYFALPIFDNSSTLYLGVISHDCFECIDAFRFACGKQIETTLVNKEELREAIFIVTGQYP
EDADDPMGKSGLEEDTLTQLEELADESALNDDLALSSEQDEAPIVRFINQMLLRAVRQRASDIHFEPYEKQYRVRFRVDG
VLQEIASPPDKLGARLAARLKIMSKLDIAEKRIPQDGRIKLKFKDTPAIDLRVSTLPTLFGEKVVLRILDSSAANLNIDQ
LGFTDADKQRYLSALAKPQGMILVTGPTGSGKTVTLYTGLSLLNQPERNISTAEDPVEINLAGINQTQINLKAGLTFASA
LRAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLANMGVPAYNIASSIHLIIAQRLARRLCP
ECRQPHLLTEAEQKAAGFSQCHAEGLRIFKPKGCPKCTNGYKGRTGLYEIMPITPALTDAILRGEHAPELTHIARQHGML
TLREAGLNKVREGVTSLEEIERVTGV

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=486822 IHE26_RS02035 WP_192437103.1 424347..425867(-) (pilB) [Plesiomonas shigelloides strain P5462]
ATGAAAATAGAAAACCCTACCGAACTAAAAAAATACGTATCCTTTTCATCACTGATTACTAAATATTTTGCCCTGCCGAT
ATTTGATAACAGTAGCACGCTATATCTTGGTGTCATCAGCCATGACTGCTTCGAGTGTATTGATGCGTTTCGCTTTGCTT
GTGGCAAACAAATTGAAACCACTCTGGTTAATAAAGAAGAACTGCGTGAGGCCATTTTCATCGTCACCGGACAATATCCG
GAAGATGCTGACGATCCAATGGGAAAATCGGGGCTAGAAGAAGACACCCTGACTCAACTTGAAGAGTTGGCGGACGAAAG
CGCGCTCAATGATGATCTAGCCTTAAGCAGTGAGCAAGATGAAGCCCCTATCGTGCGTTTTATCAACCAGATGCTGCTAC
GCGCGGTGCGTCAACGCGCCTCGGATATTCATTTTGAGCCCTATGAAAAGCAGTATCGCGTGCGCTTTCGGGTTGATGGC
GTATTGCAAGAGATCGCCAGCCCACCGGATAAACTCGGTGCTCGCTTGGCTGCACGCCTAAAAATCATGAGTAAGTTGGA
TATTGCCGAAAAGCGCATCCCGCAAGATGGCCGTATCAAGCTCAAATTTAAAGACACGCCTGCTATCGATCTGCGCGTTT
CTACGCTGCCCACCCTATTTGGGGAGAAAGTGGTACTGCGGATCCTCGACTCCTCTGCCGCCAACTTAAATATTGATCAG
CTCGGGTTTACTGATGCCGATAAACAGCGCTATTTGAGCGCGTTAGCTAAACCGCAAGGCATGATCTTAGTCACCGGCCC
GACCGGTAGTGGTAAAACCGTGACCTTGTATACCGGTTTGTCATTACTCAATCAGCCAGAGCGCAATATCTCTACCGCCG
AAGATCCGGTGGAGATCAACTTAGCCGGTATCAACCAAACACAGATCAACCTCAAAGCCGGCCTGACGTTCGCCAGCGCA
TTACGGGCCTTTTTGCGGCAAGATCCTGACGTCGTGATGGTCGGGGAGATTCGCGATCTGGAAACCGCCGAAATTGCGGT
AAAAGCGGCGCAAACCGGGCACTTGGTACTGTCGACGCTGCACACCAATTCTGCCGCCGAAACCCTCACTCGACTGGCCA
ATATGGGCGTTCCGGCCTATAACATTGCCTCATCAATTCATCTGATCATCGCTCAGCGATTGGCGCGCCGCCTCTGCCCC
GAGTGTCGCCAGCCACATCTGCTGACCGAAGCAGAACAAAAAGCGGCTGGCTTTAGCCAATGCCACGCCGAAGGATTACG
CATTTTTAAACCCAAGGGCTGCCCAAAATGTACCAACGGCTACAAAGGGCGAACCGGCTTATATGAAATTATGCCAATCA
CCCCGGCACTGACCGATGCCATTTTACGCGGTGAGCATGCGCCAGAGTTAACCCACATTGCACGCCAACATGGCATGCTG
ACCTTGCGTGAAGCAGGTCTGAATAAAGTGCGTGAAGGCGTAACATCATTGGAAGAAATTGAACGGGTTACAGGAGTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

56.687

99.012

0.561
  pilB Vibrio parahaemolyticus RIMD 2210633

57.582

96.443

0.555
  pilB Vibrio cholerae strain A1552

55.206

100

0.555
  pilB Acinetobacter baumannii D1279779

52.063

100

0.524
  pilB Acinetobacter baylyi ADP1

53.469

96.838

0.518
  pilB Legionella pneumophila strain ERS1305867

52.352

96.64

0.506
  pilF Neisseria gonorrhoeae MS11

56.298

76.877

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.604

99.802

0.395
  pilB Haemophilus influenzae Rd KW20

40.286

96.64

0.389
  pilB Haemophilus influenzae 86-028NP

40.082

96.64

0.387
  pilB Glaesserella parasuis strain SC1401

37.5

99.605

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

44.769

81.225

0.364