Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   KCG52_RS08035 Genome accession   NZ_CP073114
Coordinates   1672248..1672721 (-) Length   157 a.a.
NCBI ID   WP_036493020.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1667248..1677721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG52_RS08015 (KCG52_07995) - 1668415..1669833 (-) 1419 WP_254343664.1 sodium:proton antiporter -
  KCG52_RS08020 (KCG52_08000) - 1670274..1670633 (-) 360 WP_070615822.1 YbaN family protein -
  KCG52_RS08025 (KCG52_08005) - 1670647..1671243 (-) 597 WP_254343666.1 DUF1415 domain-containing protein -
  KCG52_RS08030 (KCG52_08010) - 1671361..1671843 (-) 483 WP_003684427.1 Lrp/AsnC family transcriptional regulator -
  KCG52_RS08035 (KCG52_08015) pilA/pilA1 1672248..1672721 (-) 474 WP_036493020.1 pilin Machinery gene
  KCG52_RS08045 (KCG52_08020) pilA2 1672828..1673298 (-) 471 WP_070615817.1 pilin Machinery gene
  KCG52_RS08050 (KCG52_08025) glnD 1674088..1676646 (-) 2559 WP_070843592.1 [protein-PII] uridylyltransferase -
  KCG52_RS08055 (KCG52_08030) - 1676666..1676962 (-) 297 WP_003680870.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16903.41 Da        Isoelectric Point: 5.0787

>NTDB_id=486814 KCG52_RS08035 WP_036493020.1 1672248..1672721(-) (pilA/pilA1) [Neisseria subflava strain HP0048]
MNTPFQAQKGFTLIELMIVIAILGILVVVAIPTYQNYIGRAQASEALYLADDLKTEISIASAVNNRLPNAEDVSKQGAIG
VTAQRIGGTYIQNGGVTVEADTGKISIPFDKGQNKGKVLTLTPYRINNDSTWLLNWQCGGTLDPMMIPAMCRDNSNG

Nucleotide


Download         Length: 474 bp        

>NTDB_id=486814 KCG52_RS08035 WP_036493020.1 1672248..1672721(-) (pilA/pilA1) [Neisseria subflava strain HP0048]
ATGAATACCCCATTTCAAGCCCAAAAAGGTTTTACCCTGATTGAGCTGATGATAGTTATCGCCATCCTCGGTATTCTGGT
TGTTGTCGCCATACCCACTTACCAAAACTATATCGGCAGGGCGCAAGCCTCCGAAGCACTGTACCTTGCCGACGACCTGA
AAACCGAAATCAGCATTGCCTCAGCGGTAAATAACCGCCTGCCCAATGCAGAGGATGTCAGCAAACAAGGTGCAATCGGC
GTTACCGCTCAACGCATCGGCGGCACATACATCCAAAATGGTGGCGTAACTGTAGAAGCAGATACAGGCAAAATCAGTAT
TCCTTTCGATAAAGGCCAAAACAAAGGCAAAGTCCTGACCCTGACCCCTTATCGAATTAACAATGACTCGACTTGGTTGC
TTAACTGGCAATGCGGCGGAACGCTGGATCCGATGATGATCCCCGCCATGTGCCGAGACAACTCAAACGGATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)

Pilin (bacterial filament) (Pilin)

(40-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

42

95.541

0.401
  pilE Neisseria gonorrhoeae MS11

35.26

100

0.389
  pilA Pseudomonas aeruginosa PAK

36.306

100

0.363
  pilA2 Legionella pneumophila str. Paris

38.255

94.904

0.363
  pilA2 Legionella pneumophila strain ERS1305867

38.255

94.904

0.363
  comP Acinetobacter baylyi ADP1

37.748

96.178

0.363