Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IHE27_RS14115 Genome accession   NZ_CP062181
Coordinates   2388744..2390267 (+) Length   507 a.a.
NCBI ID   WP_235091258.1    Uniprot ID   -
Organism   Mycetohabitans endofungorum strain B3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2383744..2395267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE27_RS14090 (IHE27_13830) - 2384934..2385917 (+) 984 WP_076786050.1 KpsF/GutQ family sugar-phosphate isomerase -
  IHE27_RS14095 (IHE27_13835) - 2385925..2386455 (+) 531 WP_076786049.1 HAD family hydrolase -
  IHE27_RS14100 (IHE27_13840) lptC 2386459..2387064 (+) 606 WP_076786048.1 LPS export ABC transporter periplasmic protein LptC -
  IHE27_RS14105 (IHE27_13845) lptA 2387128..2387796 (+) 669 WP_076787652.1 lipopolysaccharide transport periplasmic protein LptA -
  IHE27_RS14110 (IHE27_13850) lptB 2387793..2388572 (+) 780 WP_235091256.1 LPS export ABC transporter ATP-binding protein -
  IHE27_RS14115 (IHE27_13855) rpoN/rpoN1 2388744..2390267 (+) 1524 WP_235091258.1 RNA polymerase factor sigma-54 Machinery gene
  IHE27_RS14120 (IHE27_13860) raiA 2390455..2390805 (+) 351 WP_076786045.1 ribosome-associated translation inhibitor RaiA -
  IHE27_RS14125 (IHE27_13865) ptsN 2391059..2391514 (+) 456 WP_076796362.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IHE27_RS14130 (IHE27_13870) hprK 2391612..2392580 (+) 969 WP_041754506.1 HPr(Ser) kinase/phosphatase -
  IHE27_RS14135 (IHE27_13875) rapZ 2392634..2393548 (+) 915 WP_076797278.1 RNase adapter RapZ -
  IHE27_RS14140 (IHE27_13880) - 2393573..2394211 (+) 639 WP_076786042.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 55408.81 Da        Isoelectric Point: 5.1678

>NTDB_id=486815 IHE27_RS14115 WP_235091258.1 2388744..2390267(+) (rpoN/rpoN1) [Mycetohabitans endofungorum strain B3]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENEDDWIASPLRVAADGSLLPSQSSALPTGMAE
APAPVLGSANGVNGERAESAEPAAVDEYNGMNSDSGTDTASWNLDDYVRPGGTSDDDEMPPLQLHESTVSLREHLGAQLR
LTKASPRDRALVTFLIESLDDDGYLSASCDEILADLPAELEVDADELNAALALLHSFDPAGVGARSASECLKLQLLRLEP
SATRTLALEIVSNYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTSQGWQAEL
NREVVPRLRVNHLYANILRSNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQRSFFVHGEIAMRPLVLRE
IADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQ
GFVVARRTVAKYREALKIPAVSMRKSL

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=486815 IHE27_RS14115 WP_235091258.1 2388744..2390267(+) (rpoN/rpoN1) [Mycetohabitans endofungorum strain B3]
ATGAAAGCAAGCCTTCAACTCCGTCTATCGCAGCATCTGGCACTGACCCCGCAATTGCAGCAGTCGATTCGGCTACTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAGGTCGCCACGGCGATCGCGCAAAATCCTTTACTGGAGAACGAGGACGACTGGA
TCGCGAGCCCGTTGCGGGTCGCCGCGGACGGCTCACTGTTGCCGTCGCAAAGCAGCGCGCTGCCCACCGGCATGGCCGAG
GCACCAGCACCGGTGCTCGGCAGCGCCAACGGCGTGAACGGCGAGCGCGCGGAAAGCGCGGAGCCGGCCGCCGTCGACGA
ATACAACGGCATGAACAGCGACAGCGGCACCGACACGGCGTCGTGGAATTTGGACGACTATGTGCGTCCCGGTGGCACCT
CCGACGACGACGAGATGCCGCCGCTGCAGTTGCACGAATCCACGGTCAGCCTACGCGAGCATTTGGGCGCGCAGTTGCGC
CTGACCAAGGCTAGCCCGCGCGACCGCGCGCTGGTCACGTTTCTGATCGAGTCGCTGGACGACGATGGCTACCTGAGCGC
GAGCTGCGACGAAATCCTCGCCGACTTGCCGGCCGAACTTGAGGTCGACGCCGATGAGTTGAACGCGGCGCTCGCGCTGC
TGCACAGCTTCGATCCGGCTGGGGTCGGGGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAATTGTTGCGGCTGGAGCCA
AGCGCCACGCGCACGCTGGCGCTGGAAATCGTGTCGAACTACCTGGAGTTGCTGGCGGCGCGCGACTTCACGCGTCTGCG
CAAGCACCTGAAGGCCAGCGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCG
CGTACGGCAAGGCCGAGGCCGACTACGTGGTGCCGGACATCATCGTGAAGAAAACCTCACAGGGGTGGCAGGCGGAGTTG
AACCGCGAAGTCGTGCCGCGGCTGCGGGTCAACCACTTATACGCGAACATCCTGCGCAGCAACCGGGGTGACCCAGGCAG
CGGTTCGTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAACGATTTGAAACGATTTTGCGCG
TCGCACAAGCAATCGTCGAGCGTCAGCGTAGTTTTTTCGTGCATGGCGAGATCGCGATGCGCCCCTTGGTTTTACGAGAA
ATTGCTGATACGCTGGGCTTGCATGAGTCGACCGTATCGCGGGTCACGACCGGGAAGTACATGCTGACGCCCTTTGGGAC
GCTCGAATTCAAGTACTTCTTTGGCTCGCACGTGTCGACCGATACGGGGGGCGCGGCGTCATCGACCGCGATCCGCGCGC
TCATCAAGCAACTGATAGGAGCCGAAGACCCGAAAACCCCTCTTTCAGACAGCCGCATCGCTGAACTGCTGGCCGAACAG
GGCTTCGTGGTCGCACGCCGAACTGTGGCGAAATATCGCGAAGCGCTGAAGATCCCGGCTGTTAGCATGCGTAAGTCGTT
ATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-334)

Sigma-54, DNA binding domain (Sigma54_DBD)

(350-505)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.235

100

0.686