Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IF690_RS03235 Genome accession   NZ_CP061943
Coordinates   714845..716911 (+) Length   688 a.a.
NCBI ID   WP_202698813.1    Uniprot ID   -
Organism   Pseudomonas sp. SK3(2021)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 709845..721911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF690_RS03215 (IF690_03215) pilM 712075..713127 (+) 1053 WP_202698809.1 pilus assembly protein PilM Machinery gene
  IF690_RS03220 (IF690_03220) - 713127..713693 (+) 567 WP_202698810.1 PilN domain-containing protein -
  IF690_RS03225 (IF690_03225) pilO 713690..714307 (+) 618 WP_202698811.1 type 4a pilus biogenesis protein PilO -
  IF690_RS03230 (IF690_03230) - 714304..714831 (+) 528 WP_202698812.1 pilus assembly protein PilP -
  IF690_RS03235 (IF690_03235) pilQ 714845..716911 (+) 2067 WP_202698813.1 type IV pilus secretin PilQ Machinery gene
  IF690_RS03240 (IF690_03240) aroK 716916..717434 (+) 519 WP_009041824.1 shikimate kinase AroK -
  IF690_RS03245 (IF690_03245) aroB 717583..718683 (+) 1101 WP_202698814.1 3-dehydroquinate synthase -
  IF690_RS03250 (IF690_03250) - 718694..720283 (+) 1590 WP_016704448.1 AAA family ATPase -

Sequence

Protein


Download         Length: 688 a.a.        Molecular weight: 74599.59 Da        Isoelectric Point: 5.3037

>NTDB_id=485649 IF690_RS03235 WP_202698813.1 714845..716911(+) (pilQ) [Pseudomonas sp. SK3(2021)]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPIPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGAAQSAPGQPAVASGRTRAAPSAGRSIRSVDFQRG
EQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYSTY
QTDNKLTVSVRPMTSGDQQKRNTERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQAL
DLVLKTKGLDQRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDERGS
ITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNGSS
TIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEAA
SSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQSK
VVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2067 bp        

>NTDB_id=485649 IF690_RS03235 WP_202698813.1 714845..716911(+) (pilQ) [Pseudomonas sp. SK3(2021)]
ATGAACAGGATTTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGATACCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGCGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAC
CTGGGCAATGGCAATGCACGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTTGTCGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGGCGCAGAGTGCACCGGGGC
AGCCGGCTGTCGCGTCCGGGCGGACCAGGGCCGCGCCTTCCGCGGGTCGGTCGATTCGCAGCGTGGATTTCCAGCGCGGC
GAGCAGGGCGAGGGTAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAGCGCGATGGAAAGCT
CGTCCTTACCTTCGCCAAGGCTCGCCTGCCAGAGCCCTTGCGGGTCAGGCTGGACGTCAAGGATTTTGCCACCCCCGTGC
AATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCGACCTAC
CAGACGGACAACAAGCTTACGGTAAGCGTCAGGCCGATGACTTCGGGCGATCAGCAGAAACGCAACACCGAGCGCTCGGC
CTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACTTCACCA
ACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAGGCCCTC
GATCTGGTGCTCAAGACCAAGGGGCTGGACCAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGAAATCGC
GGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAAATTGCCGACCTGGAACCGCTACGGCGTGAACTGTTGCAGGTCA
ACTACGCCAAGGCCGCGGAGATTGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGTGGGTCG
ATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGACGGATTGTCAG
CCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAAGCCTGG
GCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGTTCTTCC
ACCATTGGTACGCCGGGCAGCACCAGCAGCAATTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGGCCTGGG
TATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAATCGTCT
CCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAAGCCGCC
TCTAGCGGGGCGACCTCGGTGTCCTTCAAGGAGGCTTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGATAACCG
CATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCAAGAAGA
ACGAGGTCAACGCCAAGGTCCTGATCAACGACGGGGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAAAGCAAG
GTTGTAGATAAAGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGAGAAAAA
ATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(370-435)

Secretin and TonB N terminus short domain (STN)

(296-342)

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(523-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.629

100

0.734
  comQ Acinetobacter baylyi ADP1

39.807

100

0.42
  pilQ Acinetobacter baumannii strain A118

37.954

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.954

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.301

97.529

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.381

96.948

0.372