Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IF690_RS03215 Genome accession   NZ_CP061943
Coordinates   712075..713127 (+) Length   350 a.a.
NCBI ID   WP_202698809.1    Uniprot ID   -
Organism   Pseudomonas sp. SK3(2021)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707075..718127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF690_RS03205 (IF690_03205) - 708017..709285 (+) 1269 WP_202698808.1 malic enzyme-like NAD(P)-binding protein -
  IF690_RS03210 (IF690_03210) - 709417..711858 (-) 2442 WP_202701286.1 penicillin-binding protein 1A -
  IF690_RS03215 (IF690_03215) pilM 712075..713127 (+) 1053 WP_202698809.1 pilus assembly protein PilM Machinery gene
  IF690_RS03220 (IF690_03220) - 713127..713693 (+) 567 WP_202698810.1 PilN domain-containing protein -
  IF690_RS03225 (IF690_03225) pilO 713690..714307 (+) 618 WP_202698811.1 type 4a pilus biogenesis protein PilO -
  IF690_RS03230 (IF690_03230) - 714304..714831 (+) 528 WP_202698812.1 pilus assembly protein PilP -
  IF690_RS03235 (IF690_03235) pilQ 714845..716911 (+) 2067 WP_202698813.1 type IV pilus secretin PilQ Machinery gene
  IF690_RS03240 (IF690_03240) aroK 716916..717434 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37049.48 Da        Isoelectric Point: 5.0008

>NTDB_id=485648 IF690_RS03215 WP_202698809.1 712075..713127(+) (pilM) [Pseudomonas sp. SK3(2021)]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSSVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLVANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=485648 IF690_RS03215 WP_202698809.1 712075..713127(+) (pilM) [Pseudomonas sp. SK3(2021)]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTCCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGTCGTGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGATGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGATCAGTACATTCCTTATCCGCTGGATGAGGCCGCCATCGATTTCGATGTCCTCGGCTATTCGGCGC
GCAATCCCGAGCGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCAGCCGGCTCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAACTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGGTCGCCAACCCCTTCGCTGGCATG
AGTATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.466