Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LYSZa7_RS06795 Genome accession   NZ_CP061699
Coordinates   1476067..1477767 (-) Length   566 a.a.
NCBI ID   WP_200200316.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LYSZa7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1471067..1482767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSZa7_RS06755 (LYSZa7_06755) - 1471238..1471663 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  LYSZa7_RS06760 (LYSZa7_06760) - 1471660..1472130 (+) 471 WP_003094664.1 hypothetical protein -
  LYSZa7_RS06765 (LYSZa7_06765) - 1472127..1472816 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  LYSZa7_RS06770 (LYSZa7_06770) - 1472922..1473131 (+) 210 WP_003094660.1 hypothetical protein -
  LYSZa7_RS06775 (LYSZa7_06775) yacG 1473168..1473368 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  LYSZa7_RS06780 (LYSZa7_06780) coaE 1473365..1473976 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  LYSZa7_RS06785 (LYSZa7_06785) pilD 1473973..1474845 (-) 873 WP_200200315.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  LYSZa7_RS06790 (LYSZa7_06790) pilC 1474846..1476063 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  LYSZa7_RS06795 (LYSZa7_06795) pilB 1476067..1477767 (-) 1701 WP_200200316.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LYSZa7_RS06800 (LYSZa7_06800) - 1477998..1478462 (+) 465 WP_124089607.1 pilin -
  LYSZa7_RS06805 (LYSZa7_06805) - 1478528..1479913 (+) 1386 WP_200200317.1 O-antigen ligase family protein -
  LYSZa7_RS06815 (LYSZa7_06815) nadC 1480094..1480942 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62328.19 Da        Isoelectric Point: 5.4489

>NTDB_id=484202 LYSZa7_RS06795 WP_200200316.1 1476067..1477767(-) (pilB) [Pseudomonas aeruginosa strain LYSZa7]
MNDSTSLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAENGSQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGILHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=484202 LYSZa7_RS06795 WP_200200316.1 1476067..1477767(-) (pilB) [Pseudomonas aeruginosa strain LYSZa7]
ATGAACGATTCCACGTCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTACAGAGCCGGGCGT
TGGTCGAGGTAGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCAACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAGGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGATGCCCTCGGCGATCTCGGCGACATCGATCTCGAT
GGAGTCGATGTGGGCAAGGCAGAAAACGGCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATACTCCACGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAATATCCTCAATACCACCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAG
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

56.762

99.293

0.564
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

51.878

98.763

0.512
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.533

94.17

0.495
  pilB Vibrio campbellii strain DS40M4

51.595

94.17

0.486
  pilB/pilB1 Synechocystis sp. PCC 6803

37.48

100

0.41
  pilF Thermus thermophilus HB27

38.84

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378