Detailed information NTG0483835

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC776_RS16695
• Genome accession: NZ_CP061646
• Coordinates: 3596591..3597649 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS39
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3591591..3602649

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC776_RS16670 (IC776_16670)	aroK	3591903..3592472 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC776_RS16675 (IC776_16675)	pilQ	3592484..3594610 (-)	2127	WP_191012244.1	type IV pilus secretin PilQ family protein	Machinery gene
IC776_RS16680 (IC776_16680)	pilP	3594672..3595199 (-)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC776_RS16685 (IC776_16685)	pilO	3595210..3595950 (-)	741	WP_191012245.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC776_RS16690 (IC776_16690)	pilN	3595947..3596591 (-)	645	WP_077163636.1	PilN domain-containing protein	Machinery gene
IC776_RS16695 (IC776_16695)	pilM	3596591..3597649 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC776_RS16700 (IC776_16700)	ponA	3597811..3600366 (+)	2556	WP_174722665.1	penicillin-binding protein PBP1a	-
IC776_RS16705 (IC776_16705)	-	3600384..3601202 (-)	819	WP_396021016.1	putative RNA methyltransferase	-
IC776_RS16710 (IC776_16710)	-	3601211..3601543 (-)	333	WP_191012247.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483835 IC776_RS16695 WP_004705187.1 3596591..3597649(-) (pilM) [Acinetobacter seifertii strain AS39]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483835 IC776_RS16695 WP_004705187.1 3596591..3597649(-) (pilM) [Acinetobacter seifertii strain AS39]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTACGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTTTACCTGACCGGCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGACAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426