Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC776_RS01665 Genome accession   NZ_CP061646
Coordinates   351320..353032 (-) Length   570 a.a.
NCBI ID   WP_191012503.1    Uniprot ID   A0A7H2V8F7
Organism   Acinetobacter seifertii strain AS39     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346320..358032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC776_RS01635 (IC776_01635) - 346483..346809 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  IC776_RS01640 (IC776_01640) rlmB 346912..347661 (+) 750 WP_039251214.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC776_RS01645 (IC776_01645) - 347697..348611 (+) 915 WP_049064242.1 DMT family transporter -
  IC776_RS01650 (IC776_01650) coaE 348608..349204 (-) 597 WP_191012501.1 dephospho-CoA kinase -
  IC776_RS19405 (IC776_01655) pilD 349206..350066 (-) 861 WP_191012502.1 prepilin peptidase Machinery gene
  IC776_RS01660 (IC776_01660) pilC 350066..351292 (-) 1227 WP_134262170.1 type II secretion system F family protein Machinery gene
  IC776_RS01665 (IC776_01665) pilB 351320..353032 (-) 1713 WP_191012503.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC776_RS01670 (IC776_01670) tpiA 353322..354116 (+) 795 WP_191012504.1 triose-phosphate isomerase -
  IC776_RS01675 (IC776_01675) secG 354129..354458 (+) 330 WP_002156913.1 preprotein translocase subunit SecG -
  IC776_RS01695 (IC776_01695) rimP 355096..355620 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC776_RS01700 (IC776_01700) nusA 355658..357142 (+) 1485 WP_004699068.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63492.76 Da        Isoelectric Point: 5.1596

>NTDB_id=483802 IC776_RS01665 WP_191012503.1 351320..353032(-) (pilB) [Acinetobacter seifertii strain AS39]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEAHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRIGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECRDGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483802 IC776_RS01665 WP_191012503.1 351320..353032(-) (pilB) [Acinetobacter seifertii strain AS39]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGCGCATCTCTCTC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAAACCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCACCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATAGGAGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCTAATCCGCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGGATTGACGCCCTAGGATACGAAGAAGAACAAAAAGCTTTGTTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GATTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAATAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTAGAAATGGGCTTT
ACTGAACAGGACTTAGCACATCCAGACTTTCGGGTTTTCCAACCTGTAGGTTGCCCTGAATGTCGTGATGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTAATGCAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2V8F7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

96.667

100

0.967
  pilB Acinetobacter baylyi ADP1

79.298

100

0.793
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.515

92.632

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.288

91.404

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384