Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC781_RS01735 Genome accession   NZ_CP061626
Coordinates   373027..374739 (-) Length   570 a.a.
NCBI ID   WP_191010750.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS48     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368027..379739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC781_RS01705 (IC781_01705) - 368191..368517 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  IC781_RS01710 (IC781_01710) rlmB 368620..369369 (+) 750 WP_019456777.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC781_RS01715 (IC781_01715) - 369404..370318 (+) 915 WP_191010746.1 DMT family transporter -
  IC781_RS01720 (IC781_01720) coaE 370315..370911 (-) 597 WP_191010747.1 dephospho-CoA kinase -
  IC781_RS19235 (IC781_01725) pilD 370913..371773 (-) 861 WP_191010748.1 prepilin peptidase Machinery gene
  IC781_RS01730 (IC781_01730) pilC 371773..372999 (-) 1227 WP_191010749.1 type II secretion system F family protein Machinery gene
  IC781_RS01735 (IC781_01735) pilB 373027..374739 (-) 1713 WP_191010750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC781_RS01740 (IC781_01740) tpiA 375029..375823 (+) 795 WP_191010751.1 triose-phosphate isomerase -
  IC781_RS01745 (IC781_01745) secG 375836..376165 (+) 330 WP_019456770.1 preprotein translocase subunit SecG -
  IC781_RS01765 (IC781_01765) rimP 376803..377327 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC781_RS01770 (IC781_01770) nusA 377365..378849 (+) 1485 WP_004699068.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63593.93 Da        Isoelectric Point: 5.2430

>NTDB_id=483635 IC781_RS01735 WP_191010750.1 373027..374739(-) (pilB) [Acinetobacter seifertii strain AS48]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPRDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECRDGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483635 IC781_RS01735 WP_191010750.1 373027..374739(-) (pilB) [Acinetobacter seifertii strain AS48]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGTGCATCTCTCCC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TCGAGCACCATAAACTCGAAAAAGTTTTAAACCAACATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCTCCCATTGT
TAAATATATTAATAAGTTGCTGATTGATGCGATTCGTATGGGGGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCTAACCCTCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTATCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGAATTGATGCCCTAGGATACGAAGAAGAACAAAAAGCTTTGTTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCCTTTTTACGACAAGACCCTGACATTATTATGGTTGGTGAGATTCGT
GACTTGGAAACAGCAGAAATCGCGATTAAGGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAATAGTGC
GCCTGAAACGCTGACTCGCTTACGCAATATGGGAGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTAGAGATGTTCCAAAACAAAGCCTATTAGAAATGGGCTTT
ACCGAACAGGACTTAGCACATCCAGACTTTCGGGTTTTCCAACCTGTAGGTTGCCCTGAATGTCGTGATGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTTATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGCGTGACTTCC
CTACAAGAAGTTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.018

100

0.97
  pilB Acinetobacter baylyi ADP1

79.474

100

0.795
  pilB Legionella pneumophila strain ERS1305867

51.323

99.474

0.511
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.515

92.632

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.288

91.404

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384