Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IC781_RS19235 Genome accession   NZ_CP061626
Coordinates   370913..371773 (-) Length   286 a.a.
NCBI ID   WP_191010748.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 365913..376773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC781_RS19275 - 367678..368084 (+) 407 Protein_326 tetratricopeptide repeat protein -
  IC781_RS01705 (IC781_01705) - 368191..368517 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  IC781_RS01710 (IC781_01710) rlmB 368620..369369 (+) 750 WP_019456777.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC781_RS01715 (IC781_01715) - 369404..370318 (+) 915 WP_191010746.1 DMT family transporter -
  IC781_RS01720 (IC781_01720) coaE 370315..370911 (-) 597 WP_191010747.1 dephospho-CoA kinase -
  IC781_RS19235 (IC781_01725) pilD 370913..371773 (-) 861 WP_191010748.1 prepilin peptidase Machinery gene
  IC781_RS01730 (IC781_01730) pilC 371773..372999 (-) 1227 WP_191010749.1 type II secretion system F family protein Machinery gene
  IC781_RS01735 (IC781_01735) pilB 373027..374739 (-) 1713 WP_191010750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC781_RS01740 (IC781_01740) tpiA 375029..375823 (+) 795 WP_191010751.1 triose-phosphate isomerase -
  IC781_RS01745 (IC781_01745) secG 375836..376165 (+) 330 WP_019456770.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32121.60 Da        Isoelectric Point: 7.8340

>NTDB_id=483670 IC781_RS19235 WP_191010748.1 370913..371773(-) (pilD) [Acinetobacter seifertii strain AS48]
MQDIIAYFIQNLTALYVAVSLVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHGKLTLSKPASSCPKCHQP
IRWYQNIPIVSWLMLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTIQMLWGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTAPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNENQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=483670 IC781_RS19235 WP_191010748.1 370913..371773(-) (pilD) [Acinetobacter seifertii strain AS48]
ATGCAAGACATCATTGCTTATTTTATTCAAAACCTAACTGCACTTTATGTTGCGGTTTCACTCGTGAGCCTATGTATCGG
TAGCTTTCTAAATGTAGTGATTTATCGCACTCCTAAAATGATGGAGCAAGACTGGCAACAAGAATGTCAAATGCTCCTAA
ATCCAGAGCAACCCATTATTGACCATGGGAAACTGACATTAAGCAAACCTGCTTCATCATGTCCAAAGTGTCATCAACCG
ATCCGTTGGTATCAAAATATTCCTATCGTAAGCTGGTTGATGCTAAGAGGAAAATGTGGTCATTGCCAGCATCCGATTAG
CATTCGTTATCCAGCAATTGAACTACTCACCATGTTATGTTCATTGGTGGTGGTTATGGTGTTTGGCCCGACCATACAAA
TGCTTTGGGGACTCGTCCTGACTTGGGTTTTAATTGCCCTCACCTTTATAGACTTTGATACACAACTTTTGCCTGATCGC
TTTACACTGCCTTTAGCAGCACTCGGCTTAGGCATTAACACCTTTAATATTTATACCGCGCCTAACTCAGCTATTTGGGG
TTATTTAATCGGCTTTTTATGCCTATGGATTGTGTATTACTTATTTAAAGTGATTACTGGCAAAGAAGGTATGGGCTACG
GTGACTTTAAATTGCTTGCCGCATTGGGCGCATGGATGGGGCCATTGATGCTGCCATTAATTGTGTTATTGTCATCTTTA
CTAGGTGCAATTATTGGCATCATTTTATTAAAGTTAAGAAATGAAAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCGGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAGATTTATTTAGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

96.154

100

0.962
  pilD Acinetobacter baumannii D1279779

95.455

100

0.955
  pilD Vibrio cholerae strain A1552

53.846

95.455

0.514
  pilD Vibrio campbellii strain DS40M4

51.661

94.755

0.49
  pilD Neisseria gonorrhoeae MS11

47.328

91.608

0.434