Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IC788_RS13905 Genome accession   NZ_CP061596
Coordinates   2978737..2979855 (+) Length   372 a.a.
NCBI ID   WP_191000607.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS59     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973737..2984855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC788_RS13890 (IC788_13890) - 2975903..2976745 (-) 843 WP_191000605.1 undecaprenyl-diphosphate phosphatase -
  IC788_RS13895 (IC788_13895) - 2976853..2977545 (-) 693 WP_191000606.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC788_RS13900 (IC788_13900) pilT 2977672..2978709 (+) 1038 WP_107972265.1 type IV pilus twitching motility protein PilT Machinery gene
  IC788_RS13905 (IC788_13905) pilU 2978737..2979855 (+) 1119 WP_191000607.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC788_RS13910 (IC788_13910) fur 2979925..2980362 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC788_RS13915 (IC788_13915) - 2980474..2980872 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC788_RS13920 (IC788_13920) - 2980928..2981251 (-) 324 WP_191000608.1 RnfH family protein -
  IC788_RS13925 (IC788_13925) - 2981251..2982318 (-) 1068 WP_191000609.1 hypothetical protein -
  IC788_RS13930 (IC788_13930) - 2982471..2982926 (-) 456 WP_004703295.1 bacteriohemerythrin -
  IC788_RS13935 (IC788_13935) - 2983177..2984025 (-) 849 WP_019456827.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41908.12 Da        Isoelectric Point: 6.8288

>NTDB_id=483446 IC788_RS13905 WP_191000607.1 2978737..2979855(+) (pilU) [Acinetobacter seifertii strain AS59]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSDKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAGEKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=483446 IC788_RS13905 WP_191000607.1 2978737..2979855(+) (pilU) [Acinetobacter seifertii strain AS59]
ATGGATTTTAATGACTTGCTCAACCTCATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
AATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCCA
TCATGAGTGACAAACAGCGTAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAACGGCG
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCGGGTATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTACTGTGCTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGTTGTATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCATTTGA
AATTGCTTTAAAAAACACGTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCGCGTGAAGTGATGGACT
ATGCAATTGGCTTTGCTGAAACAGGTCATCTTGTATTAGCAACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGACATAGCCAGCTCTACATGGACCTGTCGCTTAACTTAAAAGCTATGATTGCTCA
GCAGCTTATTCCAACGCCAGATGGTAATTCACGTCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAG
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGGAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(32-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

70.141

95.43

0.669
  pilU Vibrio cholerae strain A1552

54.054

99.462

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.436

97.312

0.403
  pilT Legionella pneumophila strain Lp02

39.645

90.86

0.36
  pilT Legionella pneumophila strain ERS1305867

39.645

90.86

0.36