Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC799_RS16405 Genome accession   NZ_CP061550
Coordinates   3550725..3552437 (+) Length   570 a.a.
NCBI ID   WP_151740588.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS79     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3545725..3557437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC799_RS16370 (IC799_16365) nusA 3546615..3548099 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC799_RS16375 (IC799_16370) rimP 3548137..3548661 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC799_RS16395 (IC799_16390) secG 3549299..3549628 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  IC799_RS16400 (IC799_16395) tpiA 3549641..3550435 (-) 795 WP_191012504.1 triose-phosphate isomerase -
  IC799_RS16405 (IC799_16400) pilB 3550725..3552437 (+) 1713 WP_151740588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC799_RS16410 (IC799_16405) pilC 3552465..3553691 (+) 1227 WP_134262170.1 type II secretion system F family protein Machinery gene
  IC799_RS18285 (IC799_16410) pilD 3553691..3554551 (+) 861 WP_191028695.1 prepilin peptidase Machinery gene
  IC799_RS16420 (IC799_16415) coaE 3554553..3555149 (+) 597 WP_191012501.1 dephospho-CoA kinase -
  IC799_RS16425 (IC799_16420) - 3555146..3556060 (-) 915 WP_049064242.1 DMT family transporter -
  IC799_RS16430 (IC799_16425) rlmB 3556096..3556845 (-) 750 WP_039251214.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC799_RS16435 (IC799_16430) - 3556948..3557274 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63510.80 Da        Isoelectric Point: 5.1596

>NTDB_id=483099 IC799_RS16405 WP_151740588.1 3550725..3552437(+) (pilB) [Acinetobacter seifertii strain AS79]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEAHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECRDGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483099 IC799_RS16405 WP_151740588.1 3550725..3552437(+) (pilB) [Acinetobacter seifertii strain AS79]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGCGCATCTCTCTC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAAACCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCACCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGAGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCTAATCCGCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGGATTGACGCCCTAGGATACGAAGAAGAACAAAAAGCTTTGTTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GATTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAATAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTAGAAATGGGCTTT
ACTGAACAGGACTTAGCACATCCAGACTTTCGGGTTTTCCAACCTGTAGGTTGCCCTGAATGTCGTGATGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTAATGCAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

96.842

100

0.968
  pilB Acinetobacter baylyi ADP1

79.474

100

0.795
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.515

92.632

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.288

91.404

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384