Detailed information NTG0483064

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC799_RS01555
• Genome accession: NZ_CP061550
• Coordinates: 333207..334265 (+)
• Length: 352 a.a.
• NCBI ID: WP_039251099.1
• Uniprot ID: A0A7H2P6D1
• Organism: Acinetobacter seifertii strain AS79
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 328207..339265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC799_RS01540 (IC799_01540)	-	329313..329645 (+)	333	WP_191012247.1	hypothetical protein	-
IC799_RS01545 (IC799_01545)	-	329654..330472 (+)	819	WP_396021016.1	putative RNA methyltransferase	-
IC799_RS01550 (IC799_01550)	ponA	330490..333045 (-)	2556	WP_174722665.1	penicillin-binding protein PBP1a	-
IC799_RS01555 (IC799_01555)	pilM	333207..334265 (+)	1059	WP_039251099.1	pilus assembly protein PilM	Machinery gene
IC799_RS01560 (IC799_01560)	pilN	334265..334909 (+)	645	WP_077163636.1	PilN domain-containing protein	Machinery gene
IC799_RS01565 (IC799_01565)	pilO	334906..335646 (+)	741	WP_191012245.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC799_RS01570 (IC799_01570)	pilP	335657..336184 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC799_RS01575 (IC799_01575)	pilQ	336246..338372 (+)	2127	WP_191012244.1	type IV pilus secretin PilQ family protein	Machinery gene
IC799_RS01580 (IC799_01580)	aroK	338384..338953 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38994.52 Da
• Isoelectric Point: 4.4622

>NTDB_id=483064 IC799_RS01555 WP_039251099.1 333207..334265(+) (pilM) [Acinetobacter seifertii strain AS79]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483064 IC799_RS01555 WP_039251099.1 333207..334265(+) (pilM) [Acinetobacter seifertii strain AS79]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTACGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGTCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACACGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGCGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAG
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7H2P6D1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.58	100	0.986
comM	Acinetobacter nosocomialis M2	98.295	100	0.983
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.655	100	0.429