Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC799_RS01565 Genome accession   NZ_CP061550
Coordinates   334906..335646 (+) Length   246 a.a.
NCBI ID   WP_191012245.1    Uniprot ID   A0A7H2NM85
Organism   Acinetobacter seifertii strain AS79     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329906..340646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC799_RS01550 (IC799_01550) ponA 330490..333045 (-) 2556 WP_174722665.1 penicillin-binding protein PBP1a -
  IC799_RS01555 (IC799_01555) pilM 333207..334265 (+) 1059 WP_039251099.1 pilus assembly protein PilM Machinery gene
  IC799_RS01560 (IC799_01560) pilN 334265..334909 (+) 645 WP_077163636.1 PilN domain-containing protein Machinery gene
  IC799_RS01565 (IC799_01565) pilO 334906..335646 (+) 741 WP_191012245.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC799_RS01570 (IC799_01570) pilP 335657..336184 (+) 528 WP_004705193.1 pilus assembly protein PilP Machinery gene
  IC799_RS01575 (IC799_01575) pilQ 336246..338372 (+) 2127 WP_191012244.1 type IV pilus secretin PilQ family protein Machinery gene
  IC799_RS01580 (IC799_01580) aroK 338384..338953 (+) 570 WP_004705195.1 shikimate kinase AroK -
  IC799_RS01585 (IC799_01585) aroB 338982..340064 (+) 1083 WP_191012243.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27816.67 Da        Isoelectric Point: 5.1461

>NTDB_id=483066 IC799_RS01565 WP_191012245.1 334906..335646(+) (pilO) [Acinetobacter seifertii strain AS79]
MSQDELHELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVNDSKSDIPVLNYSIKAKTYRYIGTTEAPEKLAKTPVSPAPTS
TNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483066 IC799_RS01565 WP_191012245.1 334906..335646(+) (pilO) [Acinetobacter seifertii strain AS79]
ATGAGTCAAGATGAACTTCACGAGTTATCATTAGAGCAATCGGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAACAGTTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTGAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTAGCCTTGGGTTATTTTGTTGCTATTCAGCCAAAGTTACAAGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCAGCAGTATCAAGTTCAACTTCAGGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACCGAGATTCCTAGTTTAGTTGAAGATATCAATTTAA
CGGGTGTGAACTCAGGACTGAAATTTAAAAATATTCGCTTGGAAAATGAAGTAAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCCTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCACGACTTTGTTGTAGAGGCTGCCCCTGTTAACGATAGTAAGTCAGATATTCCAGTACTGAATTATTCTATTAAGG
CGAAAACCTATCGTTATATAGGAACAACAGAGGCGCCAGAAAAACTGGCCAAAACGCCAGTATCACCTGCTCCAACTAGC
ACTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2NM85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

87.398

100

0.874
  comO Acinetobacter baylyi ADP1

74.107

91.057

0.675