Detailed information NTG0483026

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC800_RS01535
• Genome accession: NZ_CP061543
• Coordinates: 331365..332423 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS80
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 326365..337423

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC800_RS01520 (IC800_01520)	-	327472..327804 (+)	333	WP_077163639.1	hypothetical protein	-
IC800_RS01525 (IC800_01525)	-	327807..328631 (+)	825	WP_191107329.1	putative RNA methyltransferase	-
IC800_RS01530 (IC800_01530)	ponA	328648..331203 (-)	2556	WP_191107330.1	penicillin-binding protein PBP1a	-
IC800_RS01535 (IC800_01535)	pilM	331365..332423 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC800_RS01540 (IC800_01540)	pilN	332423..333067 (+)	645	WP_019457875.1	PilN domain-containing protein	Machinery gene
IC800_RS01545 (IC800_01545)	pilO	333064..333804 (+)	741	WP_191107331.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC800_RS01550 (IC800_01550)	pilP	333815..334342 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC800_RS01555 (IC800_01555)	pilQ	334404..336530 (+)	2127	WP_191107332.1	type IV pilus secretin PilQ family protein	Machinery gene
IC800_RS01560 (IC800_01560)	aroK	336542..337111 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483026 IC800_RS01535 WP_004705187.1 331365..332423(+) (pilM) [Acinetobacter seifertii strain AS80]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483026 IC800_RS01535 WP_004705187.1 331365..332423(+) (pilM) [Acinetobacter seifertii strain AS80]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCAGAAGCAGTAGCAGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACCAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCTGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGGCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGCTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCCGCTCGCTCTTTACAATTCTTCTTTTCCTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426