Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSTAA_RS03110 Genome accession   NC_017532
Coordinates   671286..673406 (+) Length   706 a.a.
NCBI ID   WP_014595708.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri DSM 4166     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 666286..678406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSTAA_RS03090 (PSTAA_0609) comM 668449..669513 (+) 1065 WP_014595704.1 pilus assembly protein PilM Machinery gene
  PSTAA_RS03095 (PSTAA_0610) pilN 669513..670085 (+) 573 WP_014595705.1 type 4a pilus biogenesis protein PilN -
  PSTAA_RS03100 pilO 670082..670708 (+) 627 WP_020307760.1 type 4a pilus biogenesis protein PilO -
  PSTAA_RS03105 (PSTAA_0612) pilP 670705..671232 (+) 528 WP_014595707.1 type 4a pilus biogenesis lipoprotein PilP -
  PSTAA_RS03110 (PSTAA_0613) pilQ 671286..673406 (+) 2121 WP_014595708.1 type IV pilus secretin PilQ Machinery gene
  PSTAA_RS03115 (PSTAA_0614) aroK 673410..673955 (+) 546 WP_017244294.1 shikimate kinase AroK -
  PSTAA_RS03120 (PSTAA_0615) aroB 673979..675082 (+) 1104 WP_014595710.1 3-dehydroquinate synthase -
  PSTAA_RS03125 (PSTAA_0616) - 675093..676688 (+) 1596 WP_014595711.1 AAA family ATPase -

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 75609.78 Da        Isoelectric Point: 4.7044

>NTDB_id=48280 PSTAA_RS03110 WP_014595708.1 671286..673406(+) (pilQ) [Stutzerimonas stutzeri DSM 4166]
MNTTLSRLGVSLLAAFISPALLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGESSGTTSPAAASAAVTAPIAKSAPVKPMAAGKAIDN
IDFRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVVFAKAELPEALRVRLDVQDFATPVKFVDSTAQAGGASIAIEPTGR
YDYLAYQTDNKLTISVKPLTEEEADKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTSNGAV
AEDRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKALGVRWGGSYTNDNWGLYGKDGA
IGRDEDGGRYLLPGTDKVGNFTLEDALAPVPFVDLGVLNSTAGIGIGYVSDHGILDLQLSAMEKTGNGEIVSQPKVVTAD
KETAKILKGAEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFANALNGVPPINKNEVNAKVLV
TDGETIVIGGVFSNSQISSIEKVPFLGDVPFVGRLFRRDIVEDKKSELLVFLTPRILNYRAVAVGQ

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=48280 PSTAA_RS03110 WP_014595708.1 671286..673406(+) (pilQ) [Stutzerimonas stutzeri DSM 4166]
ATGAACACTACCCTTTCTCGCCTGGGCGTATCTCTGCTGGCGGCATTTATATCGCCAGCACTGCTGGCGGCCAATCTCAA
CGCCTTGGATGTGGCTGCTTTGCCGGGCGATCGGGTTGAGTTGAAACTGGCATTTGACGAGCCTGTTGCGTCACCTCGTG
GCTACACGCTGGATCAGCCTGCACGCATTGCTCTGGATCTTCCAGGCGTATCCAACAACCTGGGGGCCAAAAATCGGGAG
CTGGGCGTTGGTAATGCGCGCAGCGTAACGGTCGTCGAGGCGCAAGGGCGCACCCGGCTCATCGTCAATCTGACCGCGCT
TGTTCCGTATTCCACTCGAGTGGATGGGAACAATCTGTTTGTCGTGCTGGGAGAGAGCTCGGGGACGACCAGCCCTGCGG
CTGCATCCGCCGCGGTCACTGCTCCGATTGCCAAGTCTGCTCCGGTAAAGCCGATGGCGGCCGGCAAGGCGATCGACAAT
ATCGATTTTCGCCGCGGTGAGGATGGTGCCGGAAACGTGGTAATCACACTGTCCGATCCGTCGGTAGGTTCGACTATCGA
AGAGCAGGGAGGAAAGATCCGCGTTGTGTTCGCCAAGGCGGAGCTGCCTGAAGCACTGCGTGTTCGGCTCGACGTTCAGG
ATTTCGCTACGCCGGTGAAGTTTGTCGACTCGACCGCTCAGGCAGGTGGTGCCAGTATCGCCATCGAGCCTACTGGGCGA
TACGATTATCTCGCCTACCAGACAGACAATAAGCTGACCATCAGCGTAAAGCCTCTTACAGAAGAAGAGGCCGATAAGCG
CAAGGCAGACCGTTTCGCCTATTCCGGTGAAAAACTGTCGCTGAATTTCCAAGATATTGATGTTCGTTCCGTACTGCAGT
TGATCGCTGATTTTACTGACCTGAACTTGGTTGCCAGCGATACCGTCAGTGGCAATATCACCCTTCGTTTGCAGAATGTG
CCTTGGGATCAAGCGCTCGACCTGGTTTTGAAAACCAAGGGGCTGGATAAACGGCAAGTCGGGAACGTGCTGCTCGTTGC
GCCAGCTGACGAAATCGCTGCGCGTGAACGCCAAGAGCTCGAGTCGCAGCGCCAGATTGCCGAGCTTGCACCGTTGCGTC
GTGAGGTCGTGCAAGTCAACTATGCGAAGGCTGCAGACATTGCTCGCCTGTTCCAGTCAGTGACGAGCAACGGCGCCGTC
GCAGAAGATCGTGGCTCTATAGCGGTGGATGATCGCACCAATAACATCATCGCTTATCAAACGCAGGAGCGACTTGACGA
GCTGCGTCGCATAGTCGCTCAGCTCGACATTCCTGTGCGACAAGTGATGATCGAGGCGCGGATCGTTGAGGCGAATGTGG
GCTACGACAAAGCGCTTGGGGTTCGTTGGGGGGGTAGCTACACCAACGACAATTGGGGCTTGTATGGCAAGGACGGTGCC
ATCGGTCGTGATGAAGATGGCGGACGATATCTTCTTCCAGGTACAGACAAAGTCGGCAATTTTACCCTTGAAGATGCGTT
GGCCCCGGTTCCATTTGTCGATTTAGGAGTGCTTAACAGCACAGCCGGTATTGGGATTGGCTATGTCTCGGACCATGGAA
TCCTAGACCTTCAATTGTCGGCTATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCAAAGGTGGTTACTGCGGAT
AAAGAAACGGCCAAGATCTTGAAAGGTGCGGAAATCCCCTACCAAGAAGCCAGTTCGAGTGGCGCAACGTCGACATCTTT
CAAGGAGGCAGCCCTGTCGCTTGAGGTGACGCCGCAGATCACGCCGGACAACCGCATTATCATGGAGGTCAAGGTCACCA
AGGATGCCCCTGATTTTGCTAACGCTCTGAATGGCGTGCCGCCGATCAACAAGAACGAAGTCAACGCCAAAGTTCTTGTT
ACCGATGGAGAAACAATCGTCATCGGTGGTGTATTCTCCAACAGCCAGATCAGCAGCATTGAAAAAGTGCCGTTCCTGGG
TGACGTTCCGTTCGTTGGTCGCCTCTTCCGACGGGACATCGTGGAAGATAAAAAATCCGAGCTTCTGGTCTTCTTGACCC
CTAGAATCCTCAACTACCGAGCGGTCGCCGTGGGCCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

Bacterial type II and III secretion system protein (Secretin)

(541-697)

AMIN domain (AMIN)

(38-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.074

100

0.765
  comQ Acinetobacter baylyi ADP1

41.667

98.584

0.411
  pilQ Acinetobacter baumannii strain A118

40.112

100

0.405
  pilQ Acinetobacter baumannii D1279779

40.112

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

37.647

96.317

0.363
  pilQ Legionella pneumophila strain Lp02

37.5

96.317

0.361

Multiple sequence alignment