Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PSTAA_RS03090 Genome accession   NC_017532
Coordinates   668449..669513 (+) Length   354 a.a.
NCBI ID   WP_014595704.1    Uniprot ID   A0A4S2BBV8
Organism   Stutzerimonas stutzeri DSM 4166     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 663449..674513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSTAA_RS03080 (PSTAA_0607) - 664513..665715 (-) 1203 WP_014595702.1 ISL3 family transposase -
  PSTAA_RS03085 (PSTAA_0608) - 665845..668214 (-) 2370 WP_014595703.1 penicillin-binding protein 1A -
  PSTAA_RS03090 (PSTAA_0609) comM 668449..669513 (+) 1065 WP_014595704.1 pilus assembly protein PilM Machinery gene
  PSTAA_RS03095 (PSTAA_0610) pilN 669513..670085 (+) 573 WP_014595705.1 type 4a pilus biogenesis protein PilN -
  PSTAA_RS03100 pilO 670082..670708 (+) 627 WP_020307760.1 type 4a pilus biogenesis protein PilO -
  PSTAA_RS03105 (PSTAA_0612) pilP 670705..671232 (+) 528 WP_014595707.1 type 4a pilus biogenesis lipoprotein PilP -
  PSTAA_RS03110 (PSTAA_0613) pilQ 671286..673406 (+) 2121 WP_014595708.1 type IV pilus secretin PilQ Machinery gene
  PSTAA_RS03115 (PSTAA_0614) aroK 673410..673955 (+) 546 WP_017244294.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37774.31 Da        Isoelectric Point: 4.6880

>NTDB_id=48279 PSTAA_RS03090 WP_014595704.1 668449..669513(+) (comM) [Stutzerimonas stutzeri DSM 4166]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGNRYRIEAYAVEPLPPNAVVEKNIAELEGVGQALQRVVAKAKTGCKSAVV
AVSGSAVITKMIEMDAGLSDEELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRAPGRVEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYGLLASQLGTGHGELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSLQFFFAAGQFHDVDCILLAGGTASIPGLDHMIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=48279 PSTAA_RS03090 WP_014595704.1 668449..669513(+) (comM) [Stutzerimonas stutzeri DSM 4166]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACGCTGCTTGGGATCGATATCAGTTCGACCTCAGTCAAGCTCCTCGAATT
AAGTCGATCGGGTAATCGGTACCGCATCGAGGCTTATGCCGTCGAGCCGCTACCACCGAATGCCGTCGTCGAAAAGAATA
TCGCCGAACTTGAGGGTGTCGGTCAGGCCCTGCAGCGAGTCGTTGCCAAGGCCAAGACCGGCTGCAAGTCCGCTGTTGTC
GCTGTCTCCGGGTCTGCCGTCATCACGAAGATGATCGAGATGGATGCCGGGCTTTCTGATGAAGAGCTCGAGAATCAGCT
CAAGATCGAGGCGGATCAGTACATCCCCTACCCACTGGAGGAAGTCGCGATCGACTTCGAGGTGCAAGGTCCTGCGCCTC
GCGCGCCTGGCAGGGTAGAAGTTCTCCTCGCCGCTTGCCGTAAAGAGAATGTCGAGATCCGTGAAGCCGCGCTGGCCTTG
GCCGGGCTTACCGCCAAAGTGGTGGATGTCGAGGCCTATGCGCTTGAGCGCTCCTACGGATTGCTCGCTTCACAGTTGGG
TACAGGGCACGGCGAGCTGACCGTTGCCGTCGTCGACATCGGCGCGACCATGACAACCCTGAGCGTGCTGCATAACGGGC
GTACCATCTACACGCGCGAGCAGCTGTTCGGTGGCCGCCAACTGACCGAAGAGATCCAGCGCCGCTACGGTCTGTCTCAG
GAAGAAGCCGGCCTTGCCAAGAAGCAAGGTGGTCTCCCAGATGACTACGACAGTGAAGTCCTGCTTCCGTTCAAGGAGGC
GGTGGTGCAGCAGGTATCCCGCTCGTTGCAGTTCTTCTTCGCTGCAGGGCAGTTCCACGACGTCGATTGCATCCTGCTCG
CGGGCGGAACGGCCTCCATTCCGGGCCTCGATCACATGATTCAGCAGAAGATCGGAACGCAAACTCTAGTGGCTAATCCT
TTTGCCGATATGGCTTTGAGCAGCAAGGTGAACGCCGGGGCGCTGGCCAGTGATGCGCCTGCGTTAATGATTGCATGCGG
TTTGGCGCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4S2BBV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.46

Multiple sequence alignment