Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC757_RS04660 Genome accession   NZ_CP061368
Coordinates   1066459..1068180 (-) Length   573 a.a.
NCBI ID   WP_223846256.1    Uniprot ID   -
Organism   Wenzhouxiangella sp. AB-CW3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061459..1073180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC757_RS04635 (IC757_04635) - 1061676..1062086 (-) 411 WP_190976208.1 UrcA family protein -
  IC757_RS04640 (IC757_04640) - 1062294..1062902 (-) 609 WP_190976209.1 hypothetical protein -
  IC757_RS04645 (IC757_04645) - 1062903..1063124 (-) 222 WP_190976210.1 hypothetical protein -
  IC757_RS04650 (IC757_04650) pilD 1063446..1064306 (-) 861 WP_190976211.1 prepilin peptidase Machinery gene
  IC757_RS04655 (IC757_04655) pilC 1064402..1065622 (-) 1221 WP_223846255.1 type II secretion system F family protein Machinery gene
  IC757_RS04660 (IC757_04660) pilB 1066459..1068180 (-) 1722 WP_223846256.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC757_RS04665 (IC757_04665) - 1068421..1069275 (+) 855 WP_190976212.1 glycosyltransferase family 2 protein -
  IC757_RS04670 (IC757_04670) - 1069305..1070375 (+) 1071 WP_190976213.1 DUF354 domain-containing protein -
  IC757_RS04675 (IC757_04675) - 1070379..1071287 (+) 909 WP_190976214.1 hypothetical protein -
  IC757_RS04680 (IC757_04680) - 1071280..1072191 (+) 912 WP_190976215.1 sulfotransferase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62939.87 Da        Isoelectric Point: 5.2694

>NTDB_id=482125 IC757_RS04660 WP_223846256.1 1066459..1068180(-) (pilB) [Wenzhouxiangella sp. AB-CW3]
MPAQPASAMRLPLLARKMVEAGLFDENKAQEAVETAGKKGRTFTSHIVREKLVDAMAYAHLASEDFGMPLIDLTAVDLKN
APTDAVKEELLRKHLIVPILKRGRRLFVAVADPGNKAGLDEITFTTGLQVEAVLAPYSAIEPALDRATAGSASAFNELTE
EEDNLENLEFDQGEEQQDQQQDAAGSDDAPVVRFVNKVLVDAIRKGASDIHFEPYEEEYRVRFRVDGVLVTQVRPPKRMS
PRLSARLKVMANLDIAERRIPQDGRIKLNISKGQAYDFRVSTCPTLWGEKIVLRILDSSGAYLGPEKLGFEPKQQEDYLA
AINKPYGMVLVTGPTGSGKTVTLYTALGILNDEGRNISTVEDPVEIRMKGINQVQQNTKQGLTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPASIARLTNMGVPSYNIVSTVHLVLAQRLGRTLHTCKKREEVPDDALL
RVGFTEDEVAEGIQIYEANGCDQCTAGYKGRTGVFQVMPITEDMERVILEGGSALDLEKQAAREGIVDLRRAALNKVKAG
RISLAEMNRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=482125 IC757_RS04660 WP_223846256.1 1066459..1068180(-) (pilB) [Wenzhouxiangella sp. AB-CW3]
ATGCCCGCCCAACCCGCCAGCGCCATGCGCCTACCTCTGCTGGCCCGCAAGATGGTCGAGGCCGGCCTGTTTGACGAGAA
CAAGGCGCAGGAAGCCGTCGAGACGGCTGGCAAGAAGGGGCGCACCTTCACCAGTCACATCGTGCGCGAGAAGCTGGTCG
ATGCCATGGCCTATGCCCACCTGGCCTCCGAGGATTTCGGCATGCCGCTGATCGACCTGACCGCGGTGGATCTGAAGAAC
GCCCCCACCGATGCGGTCAAGGAAGAGTTGCTGCGCAAGCACTTGATCGTGCCCATTCTCAAGCGCGGTCGGCGCCTGTT
CGTGGCAGTGGCCGACCCCGGCAACAAGGCCGGACTCGACGAGATCACCTTTACCACCGGCTTGCAGGTCGAGGCCGTGC
TGGCCCCCTACTCGGCGATCGAACCGGCCCTGGACCGGGCCACCGCCGGTTCGGCTTCGGCCTTCAACGAGCTGACCGAG
GAAGAGGACAATCTCGAAAACCTGGAATTCGACCAGGGTGAGGAGCAGCAGGATCAGCAGCAGGATGCCGCCGGCTCGGA
TGACGCCCCGGTGGTGCGCTTTGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGCGCCTCGGACATCCACTTCGAGC
CCTACGAAGAGGAGTATCGCGTCCGCTTTCGCGTGGACGGCGTACTGGTGACCCAGGTGCGCCCGCCCAAGCGCATGTCG
CCGCGACTGTCGGCGCGTCTTAAGGTCATGGCCAATCTGGATATCGCCGAGCGCCGCATTCCGCAGGATGGCCGCATCAA
GCTCAATATTTCCAAGGGCCAGGCCTACGATTTTCGTGTCTCGACCTGCCCGACACTGTGGGGCGAGAAGATCGTGCTGC
GTATTCTCGATTCCTCCGGCGCCTATCTCGGCCCCGAGAAACTGGGCTTTGAGCCCAAGCAGCAGGAAGACTACCTTGCC
GCCATCAACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGTTCCGGTAAGACGGTCACGCTCTACACCGCCCT
GGGTATACTCAACGACGAGGGCCGGAATATTTCCACCGTCGAGGATCCGGTGGAAATTCGCATGAAGGGCATCAATCAGG
TCCAGCAGAACACCAAGCAGGGCCTCACCTTTGCCGCCGCGCTGCGCTCGTTTCTGCGCCAGGATCCCGACATCATCATG
GTCGGCGAGATCCGTGACCTGGAAACGGCCGAAATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCGACGCT
GCACACCAACGACGCCCCGGCCTCGATTGCCCGACTGACCAACATGGGTGTGCCGTCCTACAATATTGTTTCCACCGTCC
ACCTGGTGCTGGCCCAGCGCCTGGGCCGTACCCTGCATACCTGCAAAAAGCGCGAGGAAGTGCCCGACGACGCCCTGCTG
CGCGTCGGCTTTACCGAGGACGAAGTGGCCGAGGGTATCCAGATCTACGAGGCCAATGGCTGCGACCAGTGCACGGCCGG
CTACAAGGGCCGGACGGGTGTGTTCCAGGTCATGCCCATCACCGAGGACATGGAACGGGTGATTCTGGAAGGCGGCTCGG
CGCTGGACCTGGAAAAGCAGGCGGCCCGCGAGGGCATCGTCGATCTGCGCCGCGCGGCGCTCAACAAGGTCAAGGCCGGA
CGCATCAGCCTGGCCGAGATGAACCGCGTAACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.323

98.953

0.508
  pilB Acinetobacter baylyi ADP1

50.536

97.731

0.494
  pilB Acinetobacter baumannii D1279779

49.821

97.731

0.487
  pilB Vibrio cholerae strain A1552

47.772

97.906

0.468
  pilF Neisseria gonorrhoeae MS11

48.893

94.59

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

46.181

98.255

0.454
  pilB Vibrio campbellii strain DS40M4

45.826

98.255

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.028

99.127

0.377
  pilF Thermus thermophilus HB27

37.173

100

0.372